We narrowed to 14,580 results for: pET

1. pET19b-DAD2 cPGFP(A165)

   Plasmid

   #165999

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   DAD2 cPGFP(A165)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET19b-HisDAD2 cPGFP(G164)

   Plasmid

   #165997

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   HisDAD2 cPGFP(G164)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET19b-rShHTL7 cPGFP(L166)

   Plasmid

   #165995

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   rShHTL7 cPGFP(L166)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET19b-DAD2 cPGFP(G164)

   Plasmid

   #165993

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   DAD2 cPGFP(G164)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETM10 with rat Δ192Annexin A11

   Plasmid

   #164498

   Purpose

   For bacterial expression of rat His-tagged Δ192Annexin A11

   Depositor

   Anni Vedeler

   Article

   Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

   Insert

   rat Δ192Annexin A11 (Anxa11 Rat)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   N-terminal truncation

   Promoter

   T7

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETM10 with rat Δ188Annexin A11

   Plasmid

   #164497

   Purpose

   For bacterial expression of rat His-tagged Δ188Annexin A11

   Depositor

   Anni Vedeler

   Article

   Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

   Insert

   rat Δ188Annexin A11 (Anxa11 Rat)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   N-terminal truncation

   Promoter

   T7

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CPH1141 (pET151_Spt6 1247-1451)

   Plasmid

   #91815

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   deleted residues 1-1246

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-Synapsin-YPET-C1-WPRE

   Plasmid

   #159954

   Purpose

   untargeted marker

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   YPET-C1
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET15-His-∆NGlpG(Y205F)

   Plasmid

   #155132

   Purpose

   Expresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15b

   Depositor

   Sinisa Urban

   Article

   Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

   Insert

   GlpG (glpG Escherichia coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Y205F, insert codes for GlpG residues 87-276

   Promoter

   T7

   Available since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETDUET-GST AO7RE Y165A

    Plasmid

    #139428

    Purpose

    GST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed tyrosine 165 to an alanine

    Promoter

    T7 promoter-1

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETDUET-GST AO7RE P199A

    Plasmid

    #139427

    Purpose

    GST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed proline 199 to an alanine

    Promoter

    T7 promoter-1

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETDUET-GST AO7RE I137A

    Plasmid

    #139319

    Purpose

    GST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed isoleucine 137 to an alanine

    Promoter

    T7 promoter-1

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-Himar-dCas9-mammalian

    Plasmid

    #138414

    Purpose

    Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar-dCas9
    

    Use

    Tags

    3xFLAG, 6xHis, and SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]

    Plasmid

    #136688

    Purpose

    Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.

    Depositor

    Colin Garvie

    Article

    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

    Insert

    Beclin-1 (BECN1 Human)
    

    Use

    Tags

    Trigger Factor

    Expression

    Bacterial

    Mutation

    DNA optimized for E. coli expression w/ three mut…

    Promoter

    T7

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-29b-3xVSVG-GSK3(1-97)

    Plasmid

    #129583

    Purpose

    Encodes VSVG tagged GSK3(1-97) for purification by nickel affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    GSK3a(1-97) (GSK3A Human)
    

    Use

    Tags

    6xHistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJUMP18-RBS-pET-MV_RN

    Plasmid

    #127014

    Purpose

    Basic Part RN- RBS+N-terminus; B0032m Ribosome Binding Site + Met-Val N-terminus start.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Insert

    Part RBS-pET-MV_RN
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDRF1-GW YPET-T2A-mTurquoise2

    Plasmid

    #118450

    Purpose

    Produces equimolar expression of YPET and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    YPET-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET24a-VHH-tev-2xTS

    Plasmid

    #117750

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP, tev cleavage site and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

    Insert

    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFS_0362_pET30-RT-CRISPR_Candidatus_Accumulibacter_sp._BA-91_ARRAY_1

    Plasmid

    #116974

    Purpose

    Expresses CaBA-91RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Candidatus Accumulibacter sp. BA-91 RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFS_0356_pET30-RT-CRISPR_Candidatus_Accumulibacter_sp._SK-02_ARRAY_1_RC

    Plasmid

    #116968

    Purpose

    Expresses CaSK-02RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1 RC, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Candidatus Accumulibacter sp. SK-02 RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge