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pME_Cp_0_6_023_psbD_5'Hom_rev_Cr

Plasmid

#235948

Purpose

5'Homology psbD reverse in the M2 position used for genome integration at the psbD locus

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

psbD 5'Homology reverse

Use

Synthetic Biology

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_6_022_rbcL_5'Hom_rev_Cr

Plasmid

#235947

Purpose

5'Homology rbcL reverse in the M2 position used for genome integration at the rbcL locus

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

rbcL 5'Homology reverse

Use

Synthetic Biology

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_010_psbD_5'Hom_Cr

Plasmid

#235788

Purpose

5' Homology psbD in the M1 position used for genome integration at the psbD locus

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

5' Homology region of the psbD locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_005_rbcL_5'Hom_Cr

Plasmid

#235786

Purpose

5' Homology rbcL in the M1 position used for genome integration at the rbcL locus

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

5' Homology region of the rbcL locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_6_006_psbH_5'Hom_rev_Cr

Plasmid

#235940

Purpose

5' Homology psbH reversee in the M2 position used for genome integration at the psbH locus

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

psbH 5'Homology reverse

Use

Synthetic Biology

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_4_022_aac6-aph2-marker

Plasmid

#235898

Purpose

aac6-aph2 tobramycin resistance gene, codon optimized for Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

aac6-aph2-marker

Use

Synthetic Biology

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_001_psbH_5'Hom_Cr

Plasmid

#235784

Purpose

5' Homology psbH in the M1 position used for genome integration at the psbH locus

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

5' Homology region of the psbH locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_Cp_0_1_004_psbA_5'Hom_Cr

Plasmid

#235785

Purpose

5' Homology psbA in the M1 position used for genome integration at the psbA locus

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

5' Homology region of the psbA locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_Cp_0_1_007_petB_5'Hom_cr

Plasmid

#235787

Purpose

5' Homology petB in the M1 position used for genome integration at the petB locus

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

5' Homology region of the petB locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pHA-MOV10

Plasmid

#177921

Purpose

Expresses MOV10 with N-terminal HA tag

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

MOV10 (MOV10 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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Strep-Tev-Avi-hZO-1

Plasmid

#236527

Purpose

For expression of ZO-1 (human) in insect cells

Depositor

Sandra Citi

Article

Spadaro et al Curr Biol. 2017 Dec 18;27(24):3783-3795.

Insert

TJP1 (TJP1 Human)

Tags

SPY Tag and StrepTag-Tev-AviTag

Expression

Insect

Mutation

WT

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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Vsx2 hR0-36 +SV40 base prom-GFP-IRES-AP

Plasmid

#225881

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

hR0-36

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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Vsx2 hR1-25 +SV40 base prom-GFP-IRES-AP

Plasmid

#225882

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

hR1-25

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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Vsx2 hR2-20 +SV40 base prom-GFP-IRES-AP

Plasmid

#225883

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

hR2-20

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vsx2 hR3-16 +SV40 base prom-GFP-IRES-AP

Plasmid

#225884

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

hR3-16

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vsx2 mR0-37 +SV40 base prom-GFP-IRES-AP

Plasmid

#225885

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

mR0-37

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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Vsx2 mR1-28 +SV40 base prom-GFP-IRES-AP

Plasmid

#225886

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

mR1-28

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vsx2 mR2-22 +SV40 base prom-GFP-IRES-AP

Plasmid

#225887

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

mR2-22

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vsx2 mR3-17 +SV40 base prom-GFP-IRES-AP

Plasmid

#225888

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

mR3-17

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET-His-E1GFP

Plasmid

#233885

Purpose

Bacterial Expression of recombinant His-tagged E1GFP

Depositor

Francesco Cardarelli

Article

De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

Insert

E1GFP

Tags

His

Expression

Bacterial

Mutation

T65S and T203Y compared to EGFP

Promoter

T7 promoter

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-mBrn2

Plasmid

#233151

Purpose

Retroviral expression of Brn2

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Brn2 (Pou3f2 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-hMyt1l

Plasmid

#233153

Purpose

Retroviral expression of Myt1l

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Myt1l (MYT1L Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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empty_pK7fWG2_IVD_FP611

Plasmid

#228371

Purpose

Empty dual expression vector for determining subcellular localization for protein of interest

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Type

Empty backbone

Tags

Green Florescent Protien

Expression

Plant

Available Since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCRISPEY-Z3-Kan

Plasmid

#232102

Purpose

Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-Z3-Hyg

Plasmid

#232101

Purpose

Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas9-EcRT-Z3-Nat

Plasmid

#232094

Purpose

Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

Z3 promoter and Z3EV transcription factor

Use

CRISPR

Expression

Yeast

Promoter

Z3

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRV∆L-5Flpo

Plasmid

#182965

Purpose

The 3rd generation of rabies vector expressing Flpo recombinase

Depositor

Ian Wickersham

Article

Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

Flpo recombinase

Use

Rabies

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAGK43

Plasmid

#222223

Purpose

Edits NPAS2 Gene.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

Eco1 RT; 112 nt donor; PAM -19 (NPAS2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPtag5-IgK-NCAN ELS

Plasmid

#219813

Purpose

Expresses His-tagged NCAN-ELS to produce recombinant protein

Depositor

Cagla Eroglu

Article

Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

Insert

IgK (Igk Mouse)

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT56

Plasmid

#218727

Purpose

Expresses N-terminally EGFP-tagged IFT56 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nozaki et al J Cell Sci. 2016 Dec 7. pii: jcs.197004.

Insert

IFT56 (TTC26 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge