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We narrowed to 16,009 results for: grna

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Showing: 3401 - 3420 of 16009 results

  1. IL1RN gRNA1

    Plasmid
    #113130
    Purpose
    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
    Depositor
    Dominik Niopek
    Publication
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    Use
    AAV, CRISPR, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6 for gRNA expression, RSV for GFP expression
    Available since
    March 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    113130-plasmid-map-sequence-id-217998

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  3. pBluescriptSKII+ U6-sgRNA(F+E) TFRC

    Plasmid
    #74709
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA
    Depositor
    Eugene Yeo
    Publication
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. pYPQAT30

    Plasmid
    #223402
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 and the sgRNA was driven by separate ZmUbi1; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-ZmUbi-gRNA scaffold 2.0-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223402-plasmid-map-sequence-id-454247

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  10. pFA0057

    Plasmid
    #131784
    Purpose
    Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.
    Depositor
    Frank Albert
    Publication
    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.
    Insert
    20mer GFP cassette guide (gGFP) and 5' sgRNA
    Expression
    Bacterial and Yeast
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    131784-plasmid-map-sequence-id-258729

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  11. pEPOR1CB0110

    Plasmid
    #117573
    Purpose
    Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Protein kinase superfamily protein {AT1G73460} in A. thaliana
    Depositor
    Nicola Patron
    Publication
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    Promoter_AtU6-26 + sa_gRNA_Protein kinase superfamily protein {AT1G73460} A. thaliana
    Expression
    Plant
    Promoter
    AtU6-26 (pICSL90002)
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    117573-plasmid-map-sequence-id-228192

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  12. pEPOR1CB0099

    Plasmid
    #117572
    Purpose
    Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Protein kinase superfamily protein {AT1G73460} in A. thaliana
    Depositor
    Nicola Patron
    Publication
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    Promoter_AtU6-26 + sa_gRNA_Protein kinase superfamily protein {AT1G73460} A. thaliana
    Expression
    Plant
    Promoter
    AtU6-26 (pICSL90002)
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    117572-plasmid-map-sequence-id-228190

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  18. pORANGE_Tubb3 GFP(Y39TAG) KI

    Plasmid
    #182678
    Purpose
    Expression of spCas9, gRNA targeting the end of Tubb3 gene and donor GFP(Y39TAG). Can be used for amber codon suppression and click chemistry labeling of endogenous beta-3 tubulin in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Tubb3-targeting gRNA and GFP(Y39TAG) donor sequence (Tubb3, Tubb3 Rat, Mouse)
    Expression
    Mammalian
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182678-plasmid-map-sequence-id-363084

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  20. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

    Plasmid
    #71828
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BACH2-sgRNA8 (BACH2 Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71828-plasmid-map-sequence-id-131023

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Showing: 3401 - 3420 of 16009 results