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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 9,777 results for: 158

Showing: 5131 - 5160 of 9777 results

  6. Dynein-2_complex

    Plasmid
    #132536
    Purpose
    Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.
    Depositor
    Andrew Carter
    Article
    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1
    Inserts
    DYNC2H1 (DYNC2H1 Human)
    DYNC2LI1 (DYNC2LI1 Human)
    WDR34 (WDR34 Human)
    WDR60 (WDR60 Human)
    DYNLL1 (DYNLL1 Human)
    DYNLL2 (DYNLL2 Human)
    DYNLRB1 (DYNLRB1 )
    DYNLRB2 (DYNLRB2 Human)
    DYNLT1 (DYNLT1 Human)
    TCTEX1D2 (TCTEX1D2 Human)
    DYNLT3 (DYNLT3 Human)
    LC8-like (LOC392067 Human)
    Use
    Tags
    8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …
    Expression
    Insect
    Mutation
    Codon optimised for Sf9 expression
    Promoter
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    132536-plasmid-map-sequence-id-270901

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  7. pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

    Plasmid
    #179905
    Purpose
    Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014
    Insert
    SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…
    Promoter
    CAG
    Available since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    179905-plasmid-map-sequence-id-351283

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  8. p(+)MV-NSE-FlagP-M502-3p

    Plasmid
    #58748
    Purpose
    Expresses recombinant measles virus with Flag-P gene tagged si2 (siP) target sequence
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Use
    Tags
    Flag
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    58748-plasmid-map-sequence-id-90880

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  9. p(+)MV-NSE-FlagP-F497A-3p

    Plasmid
    #58796
    Purpose
    Expresses recombinant measles virus with Flag-P-F497A gene tagged si2 (siP) target sequence
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Use
    Tags
    Flag
    Expression
    Mutation
    MeV-P F497A
    Promoter
    T7
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    58796-plasmid-map-sequence-id-91091

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  10. p(+)MV-NSE-FlagP-F497D-3p

    Plasmid
    #58797
    Purpose
    Expresses recombinant measles virus with Flag-P-F497D gene tagged si2 (siP) target sequence
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Use
    Tags
    Flag
    Expression
    Mutation
    MeV-P F497D
    Promoter
    T7
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    58797-plasmid-map-sequence-id-91092

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  11. ATSR

    Plasmid
    #50052
    Purpose
    expresses t.gondii VWA+TSR1 domains in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2-ATSR
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A
    Promoter
    Chick beta-actin promoter
    Available since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. proATSR

    Plasmid
    #50053
    Purpose
    Expresses T.gondii prodomain+VWA+TSR1 domains in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2-proATSR
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A
    Promoter
    chick beta-actin promoter
    Available since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. mEos-A69V-N1

    Plasmid
    #54754
    Purpose
    Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mEos A69V, V123T, T158H
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. A5TSR

    Plasmid
    #50358
    Purpose
    expresses T.gondii MIC2(67-593) domains in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2-A5TSR
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A, N357S, N463S, N470D
    Promoter
    CHICK beta-actin promoter
    Available since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. A6TSR

    Plasmid
    #50359
    Purpose
    expresses T.gondii MIC2(67-660) domains in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2-A6TSR
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A, N357S, N463S, N470D
    Promoter
    CHICK beta-actin promoter
    Available since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. MIC2

    Plasmid
    #50050
    Purpose
    expresses toxoplasma gondii MIC2 extracellular domain in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2
    Use
    Tags
    HIS, SBP, and TEV
    Expression
    Mammalian
    Mutation
    S158A, N357S, N463S, N470D
    Promoter
    CMV
    Available since
    April 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. m2ap

    Plasmid
    #50051
    Purpose
    expresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cells
    Depositor
    Timothy Springer
    Insert
    M2AP
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    starts at aa47; N127R, S158A, S177N, N357S, N463S…
    Promoter
    CMV
    Available since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. UBC32

    Plasmid
    #25213
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubc32.001.158 (UBE2K Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25213-plasmid-map-sequence-id-461309

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  19. mEmerald-TPX2-M2-C-18

    Plasmid
    #62765
    Purpose
    Localization: Centrosomes, C1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    TPX2M2 (TPX2 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    K158A, K159A, K313A, K314A, R315A Alanine mutagen…
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. 2P0E

    Plasmid
    #60109
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    C9orf95:C9orf95_dml-X01:C201158 (NMRK1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  23. mEmerald EFHC2-N-18

    Plasmid
    #54077
    Purpose
    Localization: EF-Hand Domain , Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    EFHC2 (EFHC2 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. pet15b _yein

    Plasmid
    #220226
    Purpose
    Bacterial expression of YeiN in E. coli BL21_DE3 (pLys) or similar
    Depositor
    Bernd Nidetzky
    Insert
    psug (psuG E.coli)
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    220226-plasmid-map-sequence-id-436829

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  27. pDONR221-dyrk1aa

    Plasmid
    #192741
    Purpose
    Gateway entry vector encoding zebrafish dyrk1aa
    Depositor
    Summer Thyme
    Insert
    dyrk1aa (dyrk1aa Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    Nov. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192741-plasmid-map-sequence-id-377382

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Showing: 5131 - 5160 of 9777 results