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pES001 MBP-GSK3β-HA-His

Plasmid

#154010

Purpose

Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

GSK3B (GSK3B Human)

Tags

HA tag, His6, and MBP

Expression

Bacterial

Promoter

Tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CDK4(R24C)-P2A-Hygro_Barcode

Plasmid

#170222

Purpose

Barcoded lentiviral vector to express CDK4 (R24C) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK4 (CDK4 Human)

Use

Lentiviral

Mutation

R24C

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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AAV hSYN DIO mGluR1-myc-FLAG

Plasmid

#166227

Purpose

AAV expressing cre-dependent mGluR1-myc-FLAG

Depositor

Jason Aoto

Article

Cai et al Neuron. 2021 Apr 7;109(7):1137-1149.e5.

Insert

metabotropic glutamate receptor 1 (Grm1 Mouse)

Use

AAV and Cre/Lox

Tags

Myc and FLAG

Expression

Mammalian

Promoter

human Synapsin

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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hKCNB1 L211P

Plasmid

#124487

Purpose

missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

Depositor

Federico Sesti

Article

Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

Insert

human KCNB1 (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed leucine 211 to proline

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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hKCNB1 G381R

Plasmid

#124490

Purpose

missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

Depositor

Federico Sesti

Article

Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

Insert

human KCNB1 (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed glycine at 381 to arginine

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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hKCNB1 F416L

Plasmid

#124491

Purpose

missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

Depositor

Federico Sesti

Article

Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

Insert

human KCNB1 (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed phenyalanine 416 to leucine

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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AFDN_PDZ_1

Plasmid

#103938

Purpose

Protein expression and purification of TRITHORAX PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

AFDN (AFDN Human)

Tags

GST

Expression

Bacterial

Mutation

contains AA983-1110 of AFDN

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(C128S/D156A)-EYFP

Plasmid

#35503

Purpose

AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2(C128S/D156A)-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

C128S and D156A

Promoter

Ef1a

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot9shRNA2res_AG

Plasmid

#148828

Purpose

Mammalian Expression of HsNot9-shRNA2res

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot9-shRNA2res (CNOT9 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-TK-5UTR-BsmBI-Luciferase

Plasmid

#114670

Purpose

For one-step cloning (with blue/ white screening) of a desired 5' UTR to test its impact on translation of a firefly luciferase reporter

Depositor

Lynne Postovit

Article

Jewer et al Nat Commun. 2020 May 19;11(1):2498. doi:

Insert

LacZ

Use

Luciferase

Tags

for cloning 5' UTR-Luciferase fusions

Expression

Mammalian

Promoter

lac promoter

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_LC_WT

Plasmid

#98653

Purpose

Expresses MBP-tagged FUS AA 1-163

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-CoChR-GFP

Plasmid

#59070

Purpose

AAV-mediated expression of CoChR-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CoChR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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FCK-ChrimsonR-GFP

Plasmid

#59049

Purpose

Lentiviral expression of ChrimsonR under the CaMKII promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

K176R

Promoter

CaMKII

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pmCherryC2-Gephyrin P1

Plasmid

#68820

Purpose

mCherry tagged Geph for mammalian expression

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al Proc Natl Acad Sci U S A. 2011 Jan 4;108

Insert

Gephyrin (Gphn Rat)

Tags

mCherry

Expression

Mammalian

Mutation

silent mutations on internal EcoRI site. Gephyrin…

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pl-Synapsin-YFP-NaVII-III

Plasmid

#91246

Purpose

Expresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS)

Depositor

Matthew Grubb

Article

Dumitrescu et al Front Cell Neurosci. 2016 Nov 23;10:268.

Insert

Nav1.2 (Scn2a Rat)

Use

Lentiviral

Tags

YFP

Expression

Mammalian

Mutation

The insert codes only for the intracellular loop …

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shSTING-Mmu

Plasmid

#127697

Purpose

Doxycyclin inducible shRNA knockdown of mouse STING gene

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shSTING Mmu (Sting1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Pdpy-30-miniCAFE

Plasmid

#170115

Purpose

Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

MiniCAFE

Use

CRISPR

Mutation

D8A, HNH-truncation (Δ495–609 aa) in CjCas9

Promoter

Dpy-30

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_FL_12E

Plasmid

#98652

Purpose

Expresses MBP-tagged full length phosphomimetic FUS

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

12 S/T to E

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-synaptoPAC-minWPRE

Plasmid

#153085

Purpose

Expresses synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

Depositor

Dietmar Schmitz

Article

Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

Inserts

bPAC(WT)
Synaptophysin (Syp Rat)

Use

AAV

Tags

His tag, mScarlet, and myc tag

Expression

Mammalian

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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426Gal-FUS

Plasmid

#29591

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Expression

Yeast

Available Since

July 1, 2011

Availability

Academic Institutions and Nonprofits only