We narrowed to 11,171 results for: HAL

1. pETM33_Nsp3c_SUD-M

   Plasmid

   #156470

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3c_SUD-M (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSAVED-CHAT-cbm_PCaspase

   Plasmid

   #214047

   Purpose

   Bacterial expression plasmid for SAVED-CHAT cA3 binding pocket mutant and PCaspase from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-PCaspase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   K121E, Q122A, N274A, R276E, Y285A, F286A

   Promoter

   lacUV5 promoter

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSAVED-CHAT_PCaspase-N-C-term

   Plasmid

   #214046

   Purpose

   Bacterial expression plasmid for SAVED-CHAT, PCaspase N-terminal fragment (aa 1-153), and C-terminal fragment (154-666) from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-PCaspase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   aa 1-153, 154-666

   Promoter

   lacUV5 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSAVED-CHAT-sm_PCaspase

   Plasmid

   #214048

   Purpose

   Bacterial expression plasmid for SAVED-CHAT CHAT-CHAT singlet interface mutant and PCaspase from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-PCaspase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R456E, D459K, D476K, R479E

   Promoter

   lacUV5 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJS-BCD-Strep-SAVED-CHAT

   Plasmid

   #214030

   Purpose

   Bacterial expression plasmid for strep-tagged wild-type SAVED-CHAT from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJS-BCD-Strep-dSAVED-CHAT

   Plasmid

   #214031

   Purpose

   Bacterial expression plasmid for strep-tagged catalytically dead SAVED-CHAT mutant from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   H375A, C422A

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJS-BCD-Strep-SAVED-CHAT-sm

   Plasmid

   #214033

   Purpose

   Bacterial expression plasmid for strep-tagged SAVED-CHAT CHAT-CHAT singlet interface mutant from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   R456E, D459K, D476K, R479E

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJS-BCD-Strep-PCaspase

   Plasmid

   #214034

   Purpose

   Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   PCaspase
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. fru-T2A-CsChrimson-tdTomato HDR plasmid

   Plasmid

   #141100

   Purpose

   Plasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru gene

   Depositor

   Leslie Vosshall

   Article

   Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

   Insert

   fru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm
   

   Use

   Donor template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. R619-M96-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP

    Plasmid

    #166020

    Purpose

    mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) optimized for serology

    Depositor

    Dominic Esposito

    Article

    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

    Insert

    SARS-CoV-2 RBD (S )
    

    Use

    Tags

    3C-His8-SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQE30-VRN1-R249E-R289E-R296E

    Plasmid

    #159532

    Purpose

    Expresses Arabidopsis thaliana VERNALIZATION1 mutant, with R249E, R289E and R296E mutations, in E. coli

    Depositor

    Joshua Mylne

    Article

    King et al J Biol Chem. 2013 Feb 1;288(5):3198-207.

    Insert

    VERNALIZATION1 (VRN1 Mustard Weed)
    

    Use

    Tags

    Six-Histidine tag

    Expression

    Bacterial

    Mutation

    Three charge reversal mutations, namely R249E R28…

    Promoter

    T5

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ir7f-T2A-QF2 HDR plasmid

    Plasmid

    #140942

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7f gene

    Depositor

    Leslie Vosshall

    Article

    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

    Insert

    Ir7f-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7f-right-HDR-arm
    

    Use

    Tags

    Expression

    Mutation

    4 point mutations (annotated on plasmid map) were…

    Promoter

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM33_Nsp3e_NAB

    Plasmid

    #156473

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3e_NAB (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCMV_HyPer7RgDAAO

    Plasmid

    #217653

    Purpose

    Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane

    Depositor

    Seth Parker

    Article

    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

    Insert

    HyPer7 D amino acid oxidase
    

    Use

    Lentiviral

    Tags

    Nuclear export signal

    Expression

    Mammalian

    Mutation

    Fused DAAO to the C-terminus of HyPer7 using a Gl…

    Promoter

    CMV

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFRT/TO/HIS/FLAG/HA-HNRNPU

    Plasmid

    #38068

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    HNRNPU heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A) (HNRNPU Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-Mito-Car-GECO

    Plasmid

    #100765

    Purpose

    Lentiviral tet-inducible expression of mito-targeted genetically encoded Ca2+-indicators for optical imaging

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

    Insert

    Lenti-Mito-Car-GECO
    

    Use

    Lentiviral

    Tags

    a duplex of the mitochondrial targeting signal of…

    Expression

    Mammalian

    Mutation

    R-GECO1: E163V/I166V/V174T/M176I/F222I/A302P

    Promoter

    CMV

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-Syn-Jaws-KGC-tdTomato-ER2

    Plasmid

    #153535

    Purpose

    AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the Syn promoter.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-tdTomato-ER2
    

    Use

    AAV

    Tags

    ER2, KGC, and tdTomato

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    Syn

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-CaMKII-Jaws-KGC-tdTomato-ER2

    Plasmid

    #153534

    Purpose

    AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CaMKII promoter.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-tdTomato-ER2
    

    Use

    AAV

    Tags

    ER2, KGC, and tdTomato

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    CaMKII

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFRT/TO/HIS/FLAG/HA-ALKBH5

    Plasmid

    #38073

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    ALKBH5 alkB, alkylation repair homolog 5 (E. coli) (ALKBH5 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    cDNA clone MGC:71143 IMAGE:6339184 with longer 3&…

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-miRFP680-NES-p2A-ezrin-mRuby3-IRES-Neo

    Plasmid

    #222636

    Purpose

    Ezrin activation reporter

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    miRFP680-NES-p2A-ezrin-mRuby3 (EZR Human)
    

    Use

    Lentiviral

    Tags

    mRuby3

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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