We narrowed to 11,275 results for: VARS

1. sgiRFP1

   Plasmid

   #113972

   Purpose

   Single short guide RNA targeting GATCGAGTTCGAGCCTGCGG in iRFP670 sequence

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   iRFP670 sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgiRFP2

   Plasmid

   #113973

   Purpose

   Single short guide RNA targeting GCGCGTTCTTTGGACGCGA in iRFP670 sequence

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   iRFP670 sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. sgiRFP3

   Plasmid

   #113974

   Purpose

   Single short guide RNA targeting CGTGATGTTGTACCGCTTC in iRFP670 sequence

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   iRFP670 sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. VanR - F165V

   Plasmid

   #158965

   Purpose

   Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.

   Depositor

   Michael Jensen

   Article

   et al Biotechnology Notes, Volume 1, 2020, Pag

   Insert

   VanR - F165V
   

   Expression

   Yeast

   Mutation

   Wild type VanR from Caulobacter crescentus with m…

   Promoter

   TEF1

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. VanR - F165R

   Plasmid

   #158966

   Purpose

   Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.

   Depositor

   Michael Jensen

   Article

   et al Biotechnology Notes, Volume 1, 2020, Pag

   Insert

   VanR - F165R
   

   Expression

   Yeast

   Mutation

   Wild type VanR from Caulobacter crescentus with m…

   Promoter

   TEF1

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFJ2365 - Intron GG1 - 250bp no PATC

   Plasmid

   #159883

   Purpose

   Golden Gate compatible intron with no PATCs for negative controls

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG1 - 250bp no PATC
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFJ2345 - Intron GG2 - 250bp no PATC

   Plasmid

   #159884

   Purpose

   Golden Gate compatible intron with no PATCs for negative controls

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG2 - 250bp no PATC
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFJ2366 - intron GG3 - 250bp no PATC

   Plasmid

   #159885

   Purpose

   Golden Gate compatible intron with no PATCs for negative controls

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   intron GG3 - 250bp no PATC
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. sg1

   Plasmid

   #113966

   Purpose

   Single short guide RNA targeting GTATAGCATACATTATACG

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   sg1
   

   Promoter

   U6

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQP-2376

    Plasmid

    #138974

    Purpose

    Light-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI
    

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHIs8-4b-SaDAH::G226D

    Plasmid

    #140130

    Purpose

    Expresses SaDAH::G226D variant with N-terminal 8xHis-tag in BL21 E. coli

    Depositor

    Jing-Ke Weng

    Article

    Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

    Insert

    Dechloroacutumine halogenase G226D variant from Sinomenium acutum
    

    Tags

    8x-His, TEV protease site (ENLYFQ)

    Expression

    Bacterial

    Mutation

    changed Gly226 to Asp226

    Promoter

    T7

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. MYMH 539

    Plasmid

    #138078

    Purpose

    Control vector with no degradation tag to compare against various degradation tag variants

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    J23104-mCherry::sbfI- pSEVA-331Bb
    

    Expression

    Bacterial

    Available Since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-T3-HypaCAS9-pA

    Plasmid

    #131467

    Purpose

    Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.

    Insert

    Codon optimized HypaCas9
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PKAcs-nolinker-GFP

    Plasmid

    #108579

    Purpose

    Expresses PKAcs-GFP fusion in mammalian cells

    Depositor

    Robert Hughes

    Article

    Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

    Insert

    protein kinase A, catalytic subunit
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H87Q and W196R

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. PKAcs-G12X-GFP

    Plasmid

    #108588

    Purpose

    Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

    Depositor

    Robert Hughes

    Article

    Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

    Insert

    protein kinase A, catalytic subunit
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H87Q and W196R

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. YORWΔ22Ω2

    Plasmid

    #101705

    Purpose

    GoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Type

    Empty backbone

    Use

    Yeast expression

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. YORWΔ22Ω1

    Plasmid

    #101706

    Purpose

    GoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Type

    Empty backbone

    Use

    Yeast expression

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. YORWΔ22α2

    Plasmid

    #101707

    Purpose

    GoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Type

    Empty backbone

    Use

    Yeast expression

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

19. YORWΔ22α1

    Plasmid

    #101708

    Purpose

    GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Type

    Empty backbone

    Use

    Yeast expression

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YPRCΔ15Ω2

    Plasmid

    #101709

    Purpose

    GoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Type

    Empty backbone

    Use

    Yeast expression

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge