We narrowed to 26,296 results for: Vit

1. pDisplay-GRAB-rDA3h-IRES-EGFP-CAAX

   Plasmid

   #208697

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h and a membrane-localized EGFP in mammalian cells

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA3h
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST53-LRRK2-R1441C

   Plasmid

   #25046

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Arginine 1441 mutated to Cysteine

   Available Since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]

   Plasmid

   #74102

   Purpose

   plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)

   Depositor

   Ben Szaro

   Article

   Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

   Inserts

   Xenopus laevis beta-tubulin gene promoter region and 5'UTR
   Green Lantern Green Fluorescent Protein
   Rabbit beta 1-globin gene 3'UTR
   

   Expression

   Bacterial

   Available Since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNAI-GAL4-CREB

   Plasmid

   #46769

   Purpose

   Expression of GAL4 fusion to wild type CREB

   Depositor

   Richard Maurer

   Article

   Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

   Insert

   GAL4(1-147)-CREB (Creb1, GAL4 Rat, Budding Yeast)
   

   Tags

   GAL4

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST53-LRRK2-Y1699C

   Plasmid

   #25048

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Tyrosine 1699 mutated to Cysteine

   Available Since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1-LTK-V5/HIS

   Plasmid

   #200116

   Purpose

   Expression of human LTK receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human LTK receptor tyrosine kinase (LTK Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. FRB-TagRFPT-CAAX

   Plasmid

   #87710

   Purpose

   FRB-tagged TagRFPT; targeted to plasma membrane.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

   Insert

   FRB-TagRFPT-CAAX
   

   Tags

   6xHis, C-terminal targeting sequence from Kras, T…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. ORAI1-YFP V107M

   Plasmid

   #114180

   Purpose

   ORAI1 channel with C-terminal YFP, carrying the mutation V107M in the protein 1st transmembrane domain, responsible for a constitutive activity and associated with tubular aggregate myopathy (TAM).

   Depositor

   Nicolas Demaurex

   Article

   Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

   Insert

   ORAI1-YFP V107M (ORAI1 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   V107M

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Venus-cpVenus-FLARE-Cameleon

   Plasmid

   #123350

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-Cameleon
   

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Venus-cpVenus-FLARE-ICUE

    Plasmid

    #123355

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based cAMP indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-ICUE
    

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Venus-cpVenus-FLARE-AKAR(T/A)

    Plasmid

    #123330

    Purpose

    Negative control mutant for Venus-cpVenus-FLARE-AKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-AKAR(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCPP5912

    Plasmid

    #128703

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

    Insert

    shcE-avrE1
    

    Expression

    Bacterial

    Mutation

    Three synonymous mutations Ala 362 (GCT-GCC), Ala…

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-HA-PQBP1

    Plasmid

    #25804

    Depositor

    Marius Sudol

    Article

    Tapia et al J Biol Chem. 2010 Jun 18;285(25):19391-4

    Insert

    PQBP1 (PQBP1 Human)
    

    Tags

    6xHA

    Expression

    Mammalian

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pDisplay-GRAB-rDA2h-IRES-EGFP-CAAX

    Plasmid

    #208695

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h and a membrane-localized EGFP in mammalian cells

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA2h
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. anti-GFP scFv [N86/38] with sortase tag

    Plasmid

    #204419

    Purpose

    Mammalian Expression of anti-GFP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N86/38.

    Depositor

    Jeff Lichtman

    Article

    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

    Insert

    Recombinant mouse scFv targeting GFP (Aequorea victoria)
    

    Tags

    6xHis tag and Sortase tag

    Expression

    Mammalian

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. anti-VGluT1 scFv [N28/9] with sortase tag

    Plasmid

    #204424

    Purpose

    Mammalian Expression of anti-VGluT1 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N28/9.

    Depositor

    Jeff Lichtman

    Article

    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

    Insert

    Recombinant mouse scFv targeting VGluT1 (Rattus norvegicus)
    

    Tags

    6xHis tag and Sortase tag

    Expression

    Mammalian

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. anti-PSD-95 scFv [K28/43] with sortase tag

    Plasmid

    #204422

    Purpose

    Mammalian Expression of anti-PSD-95 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K28/43.

    Depositor

    Jeff Lichtman

    Article

    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

    Insert

    Recombinant mouse scFv targeting PSD-95 (Homo sapiens)
    

    Tags

    6xHis tag and Sortase tag

    Expression

    Mammalian

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-CARMA2sh-HA

    Plasmid

    #97072

    Purpose

    Expresses CARMA2sh/CARD14 isoform 3 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-CARMA2sh-FLAG

    Plasmid

    #97071

    Purpose

    Expresses CARMA2sh/CARD14 isoform 3 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-mCherry-TSC2(AA,3Q)

    Plasmid

    #192444

    Purpose

    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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