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pET28b-DiCas7-11-His

Plasmid

#234482

Purpose

Plasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Article

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

DiCas7-11

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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FAS_pLX307

Plasmid

#98334

Purpose

Lentiviral expression of FAS

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

FAS (FAS Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLX_TRC311/NLS-Cas13d-P2A-Blast

Plasmid

#212196

Purpose

CasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40

Depositor

Stacey Edwards

Article

Wang et al Mol Cancer. 2024 May 15;23(1):101. doi:

Insert

CasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin

Use

CRISPR and Lentiviral

Tags

HA, NLS, and blasticidin S deaminase

Expression

Mammalian

Mutation

Promoter

EF-1α promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPP2R1A_WT

Plasmid

#81760

Purpose

Gateway Donor vector containing PPP2R1A , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPP2R1A (PPP2R1A Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPP2R1A_WT

Plasmid

#81944

Purpose

Gateway Donor vector containing PPP2R1A, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPP2R1A (PPP2R1A Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSH1/M-FGFR1-Fv-Fvls-E

Plasmid

#15285

Depositor

David Spencer

Article

Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

Insert

FGFR1 kinase, FKBP12v36 (Fgfr1 Mouse)

Use

Tags

HA epitope and Myristoylation-targeting domain c-…

Expression

Mammalian

Mutation

FGFR1 cytoplasmic domain FKBP12 F36V

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pDONR223_MLST8_WT

Plasmid

#82258

Purpose

Gateway Donor vector containing MLST8 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MLST8 (MLST8 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_XBP1_WT

Plasmid

#82168

Purpose

Gateway Donor vector containing XBP1 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

XBP1 (XBP1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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ACO2_pLX307

Plasmid

#98312

Purpose

Lentiviral expression of ACO2

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

ACO2 (ACO2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_WT

Plasmid

#81856

Purpose

Gateway Donor vector containing SPOP ,part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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IQGAP1_pLX307

Plasmid

#98346

Purpose

Lentiviral expression of IQGAP1

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

IQGAP1 (IQGAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA

Plasmid

#116771

Purpose

Lentiviral expression of PIK3CA

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_p.F133S

Plasmid

#81637

Purpose

Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Tags

Expression

Mutation

F133S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_p.W131G

Plasmid

#81632

Purpose

Gateway Donor vector containing SPOP , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Tags

Expression

Mutation

W131G

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_p.F102C

Plasmid

#81642

Purpose

Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Tags

Expression

Mutation

F102C

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_p.E50K

Plasmid

#81631

Purpose

Gateway Donor vector containing SPOP ,part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E50K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_p.K101I

Plasmid

#81641

Purpose

Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Tags

Expression

Mutation

K101I

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_p.W131C

Plasmid

#81643

Purpose

Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Tags

Expression

Mutation

W131C

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_p.E47A

Plasmid

#81645

Purpose

Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E47A

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_p.K134N

Plasmid

#81647

Purpose

Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Tags

Expression

Mutation

K134N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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