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pJ211_WT_med

Plasmid

#128849

Purpose

Medium copy number plasmid for expression of E. coli flagellin from its natural promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pJ211_rha_WT_hi

Plasmid

#128853

Purpose

High copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_EBFP2-ECFP

Plasmid

#118854

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

EBFP2-ECFP

Tags

His-tag

Expression

Bacterial

Mutation

EBFP1.2-I128V/V150I/ D155V/V224R and TGAGGGGLG be…

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_LSSmOrange

Plasmid

#118860

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

LSSmOrange

Tags

His-tag

Expression

Bacterial

Mutation

A44V,F84L, W145M, I165D, M167L, G202D substituti…

Available Since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_EBFP2-mTFP1

Plasmid

#118855

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

EBFP2-mTFP1

Tags

His-tag

Expression

Bacterial

Mutation

EBFP1.2-I128V/V150I/ D155V/V224R and TGAGGGGLG be…

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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MPS-1-cmyc

Plasmid

#113059

Purpose

Expresses worm Myc-tagged MPS-1 in mammalian cells

Depositor

Federico Sesti

Article

Wang et al Biophys J. 2007 Nov 1;93(9):3083-91. doi

Insert

MiRP K+ channel accessory Subunit 1 (mps-1 Nematode)

Tags

c-Myc

Expression

Mammalian

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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Intersectin SH3 domain A

Plasmid

#47396

Depositor

Peter McPherson

Article

Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

Insert

Intersectin SH3 domain A

Tags

GST

Expression

Bacterial

Mutation

SH3 domain A

Promoter

tac

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
pM2P20X

Plasmid

#108210

Purpose

Expresses MSePCNA2-PdX fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

MsePCNA1-PdX fusion protein

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pS3C

Plasmid

#108211

Purpose

Expresses SsoPCNA3-CYP101A1 fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

SsoPCNA3-CYP101A1 fusion protein

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pM3C

Plasmid

#108212

Purpose

Expresses MsePCNA3-CYP101A1 fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

MsePCNA3-CYP101A1 fusion protein

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pM1R

Plasmid

#108208

Purpose

Expresses MsePCNA1-PdR fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

MsePCNA1-PdR fusion protein

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pS1R

Plasmid

#108207

Purpose

Expresses SsoPCNA1-PdR fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

SsoPCNA1-PdR fusion protein

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-mCFP

Plasmid

#105136

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P3nmt1-mCFP

Tags

3nmt1-mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

3nmt1

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-mYFP

Plasmid

#105138

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P3nmt1-mYFP

Tags

3nmt1-mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

3nmt1

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNfs1

Plasmid

#102967

Purpose

Suppression of mNfs1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNfs1

Use

Lentiviral and RNAi

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-aVent1-pgk-hph

Plasmid

#74916

Purpose

Mammalian expresion of aVent1

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Vent1

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-aBsx-pgk-hph

Plasmid

#74913

Purpose

Mammalian expression of aBsx

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Bsx

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3C01

Plasmid

#70944

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Orc1 (Orc1 Fly)

Use

Gateway entry vector

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3F01

Plasmid

#70947

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

ine (ine Fly)

Use

Gateway entry vector

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2E06

Plasmid

#70899

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

NAAT1 (NAAT1 Fly)

Use

Gateway entry vector

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only