We narrowed to 3,922 results for: pCMV mammalian

1. AID-BE4max-P2A-GFP

   Plasmid

   #157948

   Purpose

   Mammalian expression of AID-BE4max-P2A-GFP

   Depositor

   Dali Li

   Article

   Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   AID-BE4max
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-AR1-660

   Plasmid

   #89139

   Purpose

   mammalian expression of FLAG-tagged human androgen receptor amino acid residues 1-660 in pCMV5-FLAG

   Depositor

   Elizabeth Wilson

   Insert

   hAR-1-660 (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   contains aa 1-660

   Promoter

   CMV

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

3. FLAG-hAR-508-660

   Plasmid

   #89138

   Purpose

   mammalian expression of FLAG-tagged human androgen receptor amino acid residues 508-660 in pCMV5-FLAG

   Depositor

   Elizabeth Wilson

   Insert

   hAR-508-660 (AR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   contains aa 508-660

   Promoter

   CMV

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. 24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

   Plasmid

   #128604

   Purpose

   Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Inserts

   24xMoonTag
   kinesin family member 18b (KIF18B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pcmv

   Available since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. 12xMoonTag-12xSunTag-kif18b-24xPP7

   Plasmid

   #128606

   Purpose

   Expresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Inserts

   12xMoonTag-12xSunTag
   kinesin family member 18b (KIF18B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pcmv

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA_B2AR-s-pep

   Plasmid

   #47438

   Purpose

   FRET sensor for beta2-adrenergic receptor

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

   Insert

   beta2-adrenergic receptor (ADRB2 Human)
   

   Use

   Tags

   10 nm ER/K helix, HA, mCerulean, mCitrine, and s-…

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Sept. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

7. mMib1-FLAG

   Plasmid

   #37116

   Depositor

   Martin Dorf

   Article

   Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

   Insert

   Mib1(Mus musculus mindbomb homolog 1 (Drosophila) (Mib1)) (Mib1 Mouse)
   

   Use

   Tags

   3 x FLAG

   Expression

   Mammalian

   Mutation

   ACC Kozak sequence added before ATG annotated.

   Promoter

   CMV

   Available since

   July 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. IL2R/hE-cadherin-cytotail

   Plasmid

   #45773

   Depositor

   Barry Gumbiner

   Article

   Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

   Insert

   IL2R/hE-cadherin-cytotail (IL2RA, CDH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Interleukin-2 receptor α subunit extracellular an…

   Promoter

   CMV

   Available since

   June 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ER-CanlonicSF

   Plasmid

   #178343

   Purpose

   To explore endoplasmic reticulum lactate dynamics

   Depositor

   Alejandro San Martín

   Article

   Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

   Insert

   ER-CanlonicSF
   

   Use

   Tags

   ER Retention Signal, ER Signal Exon 1, and ER Sig…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIRIGF-IKZF3

    Plasmid

    #69046

    Purpose

    expression of IKZF3 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF3 (IKZF3 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    IRES

    Available since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. nCre-ST-NLS-P2A-NLS-pSC-cCre

    Plasmid

    #207638

    Purpose

    A plasmid encoding N-terminal Cre fused to SpyTag (ST) and photocaged SpyCatcher (pSC) fused to C-terminal Cre with a nuclear localization signal (NLS) sequence

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    nCre-SpyTag-NLS-P2A-NLS-photocaged SpyCatcher-cCre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amber stop codon at SpyCatcher’s critical lysine

    Promoter

    CMV

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA_B2AR-i-pep

    Plasmid

    #47436

    Purpose

    FRET sensor for beta2-adrenergic receptor

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

    Insert

    beta2-adrenergic receptor (ADRB2 Human)
    

    Use

    Tags

    10 nm ER/K helix, HA, i-pep, mCerulean, and mCitr…

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pCDNA_B2AR-no-pep

    Plasmid

    #47439

    Purpose

    control FRET sensor for beta2-adrenergic receptor

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

    Insert

    beta2-adrenergic receptor (ADRB2 Human)
    

    Use

    Tags

    10 nm ER/K helix, HA, mCerulean, mCitrine, and no…

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

14. NLS-nCas9-NLS-P2A-EGFP (pRZ70)

    Plasmid

    #123616

    Purpose

    CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    NLS-nCas9-NLS-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPKm-105

    Plasmid

    #104853

    Purpose

    pcDNA3 - pCMV - PhyB NT - GBD, expressing Phytochrome B (PhyB) with Gal Binding Domain (GBD), under CMV promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    PhyB NT GBD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA_B2AR-q-pep

    Plasmid

    #47437

    Purpose

    FRET sensor for beta2-adrenergic receptor

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

    Insert

    beta2-adrenergic receptor (ADRB2 Human)
    

    Use

    Tags

    10 nm ER/K helix, HA, mCerulean, mCitrine, and q-…

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

17. cmv hTid Short H121Q

    Plasmid

    #13710

    Depositor

    Karl Munger

    Article

    Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

    Insert

    hTid Short H121Q (DNAJA3 Human, hTid Short H121Q)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H121Q

    Promoter

    Available since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

18. cmv hTid Long H121Q

    Plasmid

    #13708

    Depositor

    Karl Munger

    Article

    Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

    Insert

    hTid Long H121Q (DNAJA3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H121Q

    Promoter

    Available since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

19. p5M-PR-A

    Plasmid

    #89124

    Purpose

    mammalian expression of full-length human progesterone receptor-A in pCMV5 with 3 restriction sites deleted in polylinker

    Depositor

    Elizabeth Wilson

    Article

    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

    Insert

    human progesterone receptor-A (PGR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pU6-SasgRNA scaffold-CMV-nSaCas9

    Plasmid

    #171695

    Purpose

    Expresses nSaCas9 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    sgRNA scaffold-nSaCas9-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    U6 and CMV

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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