We narrowed to 9,597 results for: EPO
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Plasmid#203539PurposeAAV reporter genome plasmidDepositorInsertNLS-mScarlet-2A-mScarlet
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDONR-53BP1 1-1972 WT
Plasmid#53446PurposeRecombinational donor/master vectorDepositorInsert53BP1 (TP53BP1 Human)
UseGatewayTagsExpressionMutationsiRNA resistant and mutations A128V, T720A, A1347…PromoterAvailable sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR-GAL4-IL10-IRES-mCherry-PGK-BFP
Plasmid#223209PurposeLentiviral vector - mCherry reporter and IL10 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorInsertIL10 (IL10 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pErCas12a EZ Clone
Plasmid#132641PurposeExpresses ErCas12a and an sgRNA to target a region of interestDepositorInsertErCas12a
UseAAV and CRISPRTags3x HA and SV40 NLSExpressionMammalianMutationQ926R (Please see depositor comments) and BsaI si…PromoterCMV PromoterAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMS2280
Plasmid#111901PurposessAAV genome with Ple345 (NEFL MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertPle345 (NEFL Human)
UseAAVTagsExpressionMutationPromoterPle345 (NEFL MiniPromoter)Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.2-YFP-CWT
Plasmid#176689PurposeFluorescent reporter for phagosomal escape of Staphylococcus aureusDepositorInsertcell wall-targeting domain of Lysostaphin
UseTagsYFPExpressionMammalianMutationdeletion of protase domain of LysostaphinPromoterCMVAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-Maroon-P2A-3xnls-Tq-Ca-FLITS
Plasmid#145027PurposeLentiviral vector for a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes - co-expresses a nuclear markerDepositorInsert3xnls-Tq-Ca-FLITS
UseLentiviralTags3xnls-Tq-Ca-FLITS and H2B-MaroonExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-APPSw;T719N
Plasmid#177094PurposeFor lentiviral transduction of APPsw; T719NDepositorInsertAPPsw;T719N (APP Human)
UseLentiviralTagsExpressionMutationT719N, K651N and M652L (please see depositor comm…PromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEMS2241
Plasmid#111896PurposessAAV genome with Ple320 (POU4F1 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertPle320 (POU4F1 Human)
UseAAVTagsExpressionMutationPromoterPle320 (POU4F1 MiniPromoter)Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
hum alpha ENAC promoter a25-a23
Plasmid#83427Purposepromoter reporter construct to test 5' flanking sequencesDepositorInserthuman aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)
UseLuciferaseTagsLuciferaseExpressionMutationPromoteralpha ENACAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSynI-rtTAV16
Plasmid#102367PurposeAAV vector to drive TET-ON activator expression from human synapsin I promoterDepositorInsertrtTAV16
UseAAVTagsExpressionMammalianMutationrtTA variant reported in Gene Therapy (2006) 13, …Promoterhuman synapsin I promoterAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-UAS-activemTGFb1-IRES-mCherry-PGK-BFP
Plasmid#223213PurposeLentiviral vector - mCherry reporter and TGFb1 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorInsertTGFb1 active - membrane (TGFB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT410 AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA
Plasmid#202059PurposeInducible AAV activatorDepositorInsertAAV-Rapamycin Inducible-NFZ-Citrine with bGH pA
UseAAV; Aav with reporterTagsExpressionMammalianMutationITRs intactPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA
Plasmid#105000PurposeExpresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeatsDepositorInsertRenilla luciferase
UseTags24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …ExpressionBacterial and MammalianMutationPromoterTetCMVAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-hygro-STING HAQ
Plasmid#102600PurposeExpress STING HAQ for IFN-beta Luciferase reporter assay.DepositorInsertSTING (STING1 Human)
UseRetroviralTagsExpressionMammalianMutationR71H-G230A-R293Q (HAQ)PromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_sgRNA_puro-mKate-lox5171
Plasmid#162077Purpose3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistanceDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Lyso-FLAG-GFP-Sac1-Cat-WT
Plasmid#134645Purposelysosome-targeted Sac1 catalytic domain wild typeDepositorInsertSac1 catalytic domain (77-520 aa) (SACM1L Human)
UseLentiviralTagsFLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…ExpressionMammalianMutationcontains only amino acids 77-520 (Please see depo…PromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-IL35-IRES-mCherry-PGK-BFP
Plasmid#223210PurposeLentiviral vector - mCherry reporter and IL35 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorInsertIL35 (IL12A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 BA.2
Plasmid#212487PurposeEncodes SARS-CoV-2 variant BA.2 Spike for pseudovirus productionDepositorInsertSARS-CoV-2 Spike BA.2 (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationSee Depositor Comments.PromoterCAGAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS
Plasmid#134653Purposelysosome-targeted Sac1 catalytic domain C389SDepositorInsertSac1 catalytic domain (77-520 aa) CS mutant (SACM1L Human)
UseLentiviralTagsFLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…ExpressionMammalianMutationC389S and contains only amino acids 77-520 (Pleas…PromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-PDL1-IRES-mCherry-PGK-BFP
Plasmid#223208PurposeLentiviral vector - mCherry reporter and PDL1 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorInsertPDL1 (CD274 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
TP63-T2A-Rox-EF1a-Rox-Venus-NLS repair template
Plasmid#167971PurposeTp63 reporter targeting repair templateDepositorInsertTp63 (TP63 Human)
UseCRISPR; Donor templateTagsVenusExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-FLEX-XRI-FLAG
Plasmid#178057PurposeExpression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.DepositorInsertXRI-FLAG
UseAAVTagsFLAG-MBPExpressionMammalianMutationPromoterHuman ubiquitin C (UBC) promoterAvailable sinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP
Plasmid#58959Purposelentiviral expression of v-H-Ras and luciferase/EGFP reporterDepositorInsertsCMV enhancer/chicken beta actin promoter
v-H-Ras
luc2/EGFP fusion gene
UseLentiviralTagsExpressionMammalianMutationPromoterCMV enhancer/chicken beta actinAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE
Plasmid#85453PurposeLentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.DepositorInsertU6-sgRNA-Backbone-UP-TdTomato
UseCRISPR and LentiviralTagsNLSExpressionMammalianMutationdeletion of AA123-365 in tdTomato (please see dep…PromoterAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(MapERK)d1EGFP (KroxDs)
Plasmid#214912Purposefor PiggyBac mediated integration and stable expression of destabilised EGFP as a reporter to MAPK/ERK pathway activationDepositorInsertd1EGFP under the MapERK promoter
UseTagsExpressionMammalianMutationPromoterMapkERK promoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-PytR(PyOtR)-mCherry
Plasmid#204870PurposeExpresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertM. mazei pyrrolysyl tRNA mutant "PyOtR"
UseAAVTagsExpressionMammalianMutationU25C; A3G, A4C, A5G, C6G, G63C, T65G, T66CPromoterU6Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastbac1-GST-hUSP7
Plasmid#63573PurposeExpression of synthetic human USP7 tagged with GSTDepositorInsertUSP7 (USP7 Synthetic, Human)
UseTagsGSTExpressionInsectMutationSynthetic based on human protein sequence (please…PromoterPolyhedrinAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Halo-p58
Plasmid#166896Purposemammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with HaloTagDepositorInsertp58 (Lman1 Rat)
UseTagsHaloTagExpressionMammalianMutationL405Q, R419S- please see depositor commentsPromoterCMVAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 HK.3
Plasmid#212572PurposeEncodes SARS-CoV-2 spike for HK.3 pseudovirus productionDepositorInsertSARS-CoV-2 Spike HK.3 (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationSee Depositor Comments.PromoterCAGAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanCOL2A1enhancer
Plasmid#173961PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertEnhancer region from COL2A1 gene
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM6-4F2-mCherry
Plasmid#73421PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.DepositorInsertPromoter 4F2 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP4F2 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTBL210
Plasmid#126451PurposeExpresses Cas9-XTEN-TdT in mammalian cells.DepositorInsertCas9-XTEN-TdT (DNTT Human, Streptococcus pyogenes)
UseTagsExpressionMammalianMutationSee depositor comments belowPromoterCMVAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRaGE Pyl TAG GFP Y35TAG
Plasmid#160041PurposeEncodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.DepositorInsertsPyrrolysyl tRNA synthetase (Methanosarcina mazei)
Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
deGFP
UseSynthetic BiologyTagsHis-tagExpressionBacterialMutationChanged tyrosine 35 to TAG stop-codon (Site numbe…PromoterP70b promoter and T500 terminator, Vibrio natrieg…Available sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-CBA-hGBA1-cm-hGBA1-hGHpA
Plasmid#218795PurposeAAV reporter genome plasmidDepositorInsertCMV-CBA-hGBA1-cm-hGBA1-hGHpA
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEMS2242
Plasmid#111897PurposessAAV genome with Ple321 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertPle321 (TUBB3 Human)
UseAAVTagsExpressionMutationPromoterPle321 (TUBB3 MiniPromoter)Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45
Plasmid#173946PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertSOX9 enhancer cluster EC1.45
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-29a-3p
Plasmid#103391PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-29a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-29a-3p target (MIR29A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTNwt-FL
Plasmid#110217PurposeExpression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsExpressionBacterialMutationP296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v6 (-cyt)
Plasmid#137817Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v6 (-cyt) (CD44 Human)
UseTagsGFPExpressionMammalianMutationwithout cytoplasmic region, R296K (please see dep…PromoterPGKAvailable sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA
Plasmid#97308PurposeHMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.DepositorInsertActb HMEJ donor
UseAAV and Mouse TargetingTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-29a-5p
Plasmid#103392PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-29a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-29a-5p target (MIR29A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT311 AAV-Rapamycin Inducible-NZF-Citrine
Plasmid#202060PurposeInducible AAV activatorDepositorInsertAAV-Rapamycin Inducible-NZF-Citrine
UseAAV; Aav with reporterTagsExpressionMammalianMutationITR deletionPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.TRE.mArid3a3xFLAG.IRES.dTomato.PGK.sfGFP.P2A.Tet3G
Plasmid#174666PurposeDoxycycline-inducible expression of murine Arid3a-3xFLAG cDNA with dTomato as reporter fluorescent proteinDepositorInsertArid3a-3xFLAG (Arid3a Mouse)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterTREAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.ARID3a.IRES.GFP
Plasmid#169300PurposeConstitutive overexpression of human ARID3A cDNA with GFP as reporter fluorescent proteinDepositorInsertARID3A (ARID3A Human)
UseLentiviralTagsIRES-EGFPExpressionMutationPromoterSFFVAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irC6kwh
Plasmid#44725DepositorInsertspCMV-D2i promoter
mCherry
UseSynthetic Biology; Expression regulator/reporterTagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA
Plasmid#97311PurposeMMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.DepositorInsertActb MMEJ donor
UseAAV and Mouse TargetingTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDN-T2dMFot
Plasmid#44558DepositorInsertsPTETREG promoter
rtetR-M2::FFF
UseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationCompared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…PromoterPTETREGAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L 2xHis-LRRK2 Armadillo 1-159
Plasmid#186016Purpose2xHis-LRRK2 Armadillo 1-159DepositorInsert2xHis-LRRK2 Armadillo 1-159 (LRRK2 Human)
UseTagsExpressionBacterialMutationSee depositor's comments below.PromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TUBG1
Plasmid#171967PurposepGEX2T-TUBG1 bacterial expression plasmid for production of recombinant GST fusion proteinDepositorInsertTUBG1 (TUBG1 Human)
UseTagsGSTExpressionBacterialMutationR275H (Please see depositor comments)PromoterAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only