We narrowed to 5,124 results for: PEP

1. hPDI1_18-508_WT_pFLAG-CMV1

   Plasmid

   #31382

   Depositor

   David Ron

   Article

   Zito et al Mol Cell. 2010 Dec 10. 40(5):787-97.

   Insert

   hPDI1 (P4HB Human)
   

   Use

   Tags

   FLAG M2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-OTI-TCRA_p2_TCRB

   Plasmid

   #131033

   Purpose

   Expresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptide

   Depositor

   Jeff Hammerbacher

   Article

   Aksoy et al bioRxiv

   Inserts

   TCRA (Tcra Mouse)
   TCRB (Tcrb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/T7

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-OTI-TCRB_p2_TCRA

   Plasmid

   #131034

   Purpose

   Expresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptide

   Depositor

   Jeff Hammerbacher

   Article

   Aksoy et al bioRxiv

   Inserts

   TCRB (Tcrb Mouse)
   TCRA (Tcra Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/T7

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCI-neo Flag HAUSP

   Plasmid

   #16655

   Depositor

   Bert Vogelstein

   Article

   Cummins et al Cell Cycle. 2004 Jun . 3(6):689-92.

   Insert

   HAUSP (USP7 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNM262

   Plasmid

   #128412

   Purpose

   Vanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.

   Depositor

   Christopher Voigt

   Article

   Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

   Insert

   Lysostaphin (epr Staphylococcus simulans)
   

   Use

   Synthetic Biology

   Tags

   amyE SP

   Expression

   Bacterial

   Mutation

   Promoter

   Pspank(V)

   Available since

   Sept. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIBA2-Int-SpyTag

   Plasmid

   #198038

   Purpose

   Expression plasmid for producing SpyTagged stalled secretion variant of intimin

   Depositor

   Jack Leo

   Article

   Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

   Insert

   eaeA
   

   Use

   Tags

   SpyTag

   Expression

   Bacterial

   Mutation

   lacks native signal peptide (amino acids 1-39)

   Promoter

   tet

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE30-butelase1

   Plasmid

   #115430

   Purpose

   Expresses the macrocyclizing asparaginyl endopeptidase butelase 1 in E. coli

   Depositor

   Joshua Mylne

   Article

   James et al Plant J. 2019 Feb 21. doi: 10.1111/tpj.1

   Insert

   butelase 1
   

   Use

   Tags

   6-His

   Expression

   Mutation

   Promoter

   T5

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEFIRES-P-mTagBFP-KDEL

   Plasmid

   #87163

   Purpose

   Fluorescent marker of endoplasmic reticulum (ER)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   mTagBFP-KDEL
   

   Use

   Tags

   Bip signal peptide (Homo sapiens) and mTagBFP-KDE…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1 alpha

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-GFP-P2A

   Plasmid

   #80807

   Purpose

   GFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptide

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   GFP-P2A
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. huntingtin_FL_NbioCflag

    Plasmid

    #194767

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    Avi-tag (biotin acceptor peptide) and Flag-tag

    Expression

    Mammalian

    Mutation

    Q23 (polyQ repeat)

    Promoter

    CMV and P10

    Available since

    July 27, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pCRISPR-S12

    Plasmid

    #84031

    Purpose

    To episomally express codon optimized Cas9 and chimeric guide RNA

    Depositor

    Sen Wu

    Article

    Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

    Inserts

    hSpCas9
    eGFP
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and CMV (downstream of F2A self-cleaving pept…

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-BCA-Q11

    Plasmid

    #185781

    Purpose

    Encodes enzyme bovine carbonic anhydrase linked to Q11 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    BCA-Q11
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNICE-LAP-neurexin-1beta

    Plasmid

    #42575

    Depositor

    Alice Ting

    Article

    Liu et al ACS Nano. 2012 Dec 21;6(12):11080-7. doi

    Insert

    LAP-neurexin-1beta
    

    Use

    Tags

    c-Myc, after signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-2mCh-PS

    Plasmid

    #112268

    Purpose

    IDOCKS Acceptor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    two copies of mCherry followed by a pseudosubstrate peptide for PKC
    

    Use

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSkipFlox

    Plasmid

    #85797

    Purpose

    P. falciparum plasmid for expression of both diCre fragments from single casette using skip peptide

    Depositor

    Tobias Spielmann

    Article

    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    split Cre recombinase
    

    Use

    Cre/Lox; P. falciparum expression

    Tags

    FKBP, FRB*

    Expression

    Mutation

    Promoter

    Pfcrt

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lyn-R-GECO1

    Plasmid

    #120410

    Purpose

    Expresses a fusion between myristoylation signal peptide and R-GECO1. This construct targets R-GECO1 to the plasma membrane.

    Depositor

    Won Do Heo

    Article

    Kim et al Proc Natl Acad Sci U S A. 2016 May 24;11

    Insert

    Lyn-R-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MXS_P2A

    Plasmid

    #62433

    Purpose

    MXS_chaining vector with P2A

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Self-cleaving peptide
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. huntingtin_V2095-V3138_NbioCflag

    Plasmid

    #194765

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    Avi-tag (biotin acceptor peptide) and Flag-tag

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV and P10

    Available since

    July 26, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pEG476

    Plasmid

    #40198

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (S404A) (mex-5 Nematode)
    

    Use

    Tags

    Dendra2 PAFP (sequence optimized for worm express…

    Expression

    Worm

    Mutation

    S404A

    Promoter

    pie-1

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pOCC82

    Plasmid

    #118862

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for untagged recombinant protein

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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