We narrowed to 9,512 results for: try

1. Lenti-XYLT2-sgRNA

   Plasmid

   #154862

   Purpose

   Lentiviral expression of Cas9 and sgRNA targeting XYLT2

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   XYLT2 sgRNA (XYLT2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCIS-rPALcc

   Plasmid

   #145804

   Purpose

   expresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cells

   Depositor

   Richard Mains, Betty Eipper

   Article

   Kolhekar et al Biochemistry. 2002 Oct 15;41(41):12384-9

   Insert

   rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
   

   Expression

   Mammalian

   Mutation

   changed serine 767 to alanine to prevent glycosyl…

   Promoter

   CMV

   Available Since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. IF405: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-p300core

   Plasmid

   #121825

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-dCas9/p300core (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. IP803: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-Dnmt3a3L

   Plasmid

   #121826

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-dCas9/Dnmt3a-3L (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. KO602: pMVP (L3-L2) HA tag + pA; CMV::TETa-pA

   Plasmid

   #121802

   Purpose

   pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven TETa

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   HA epitope tag-polyA + CMV::TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. KA601: pMVP (L3-L2) pA; EF1a::TETa-pA

   Plasmid

   #121805

   Purpose

   pMVP L3-L2 entry plasmid, contains polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven TETa downstream of gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   polyA + EF1a::TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. KA401: pMVP (L3-L2) pA; RIP::TETa-pA

   Plasmid

   #121809

   Purpose

   pMVP L3-L2 entry plasmid, contains polyA + RIP-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven TETa downstream of gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   polyA + RIP::TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. KO502: pMVP (L3-L2) HA tag + pA; RIP::TETa-pA

   Plasmid

   #121810

   Purpose

   pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven TETa

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   HA epitope tag-polyA + RIP::TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. IA304: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-VPR

   Plasmid

   #121822

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-dCas9/VPR (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. IB801: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-KRAB

    Plasmid

    #121823

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-dCas9/KRAB (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. KZ101: pMVP (L3-L2) P2A-eCFP + polyA

    Plasmid

    #121768

    Purpose

    pMVP L3-L2 entry plasmid, contains eCFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eCFP linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-eCFP + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. KZ301: pMVP (L3-L2) P2A-Blast + polyA

    Plasmid

    #121779

    Purpose

    pMVP L3-L2 entry plasmid, contains Blast-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Blast selection marker linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-Blast + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. KZ201: pMVP (L3-L2) P2A-Hygro + polyA

    Plasmid

    #121782

    Purpose

    pMVP L3-L2 entry plasmid, contains Hygro-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Hygro selection marker linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-Hygro + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. LA401: pMVP (L3-L2) P2A-Blast + WPRE

    Plasmid

    #121784

    Purpose

    pMVP L3-L2 entry plasmid, contains Blast-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Blast selection marker linked by P2A to gene in lentivirus vectors

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-Blast + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. LA501: pMVP (L3-L2) P2A-Neo + WPRE

    Plasmid

    #121786

    Purpose

    pMVP L3-L2 entry plasmid, contains Neo-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Neo selection marker linked by P2A to a gene in lentivirus vectors

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-Neo + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. LA701: pMVP (L3-L2) P2A-Zeo + WPRE

    Plasmid

    #121788

    Purpose

    pMVP L3-L2 entry plasmid, contains Zeo-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Zeo selection marker linked by P2A to a gene in lentivirus vectors.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-Zeo + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. KN401: pMVP (L3-L2) FKBP DD + WPRE

    Plasmid

    #121792

    Purpose

    pMVP L3-L2 entry plasmid, contains FKBP DD + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term FKBP degron domain (stabilized by SHLD1) to gene in lentivirus vectors.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    FKBP Degron Domain + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. KN501: pMVP (L3-L2) ecDHFR DD + WPRE

    Plasmid

    #121793

    Purpose

    pMVP L3-L2 entry plasmid, contains ecDHFR DD + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term ecDHFR degron domain (stablized by TMP) to gene in lentivirus vectors

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    ecDHFR Degron Domain + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. IX503: pMVP (L3-L2) OLLAS epitope tag + polyA

    Plasmid

    #121752

    Purpose

    pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    OLLAS epitope tag + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. IX401: pMVP (L3-L2) 6x His epitope tag + polyA

    Plasmid

    #121754

    Purpose

    pMVP L3-L2 entry plasmid, contains 6x His epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    6x His epitope tag + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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