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pDGB1_alpha2R

Plasmid

#68227

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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AlphaE

Plasmid

#124527

Purpose

pColE1_AlphaE

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial, Insect, and Mamm…

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-14

Plasmid

#119993

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeptune2.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeptune2.5

Use

Pcr template

Tags

mNeptune2.5

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_TT miRc2 3xFLAG

Plasmid

#83274

Purpose

Empty entry vector for cloning inducible mammalian expression and an in-frame N-terminal 3xFLAG

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Type

Empty backbone

Use

Gateway Cloning

Tags

3xFLAG

Promoter

TRE tight

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-24

Plasmid

#120003

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with TAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TAP

Use

Pcr template

Tags

TAP

Promoter

None

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-10

Plasmid

#119989

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mKOk

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKOk

Use

Pcr template

Tags

mKOk

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-33

Plasmid

#119986

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with FLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

FLAG

Use

Pcr template

Tags

FLAG

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pX330A_dCas9-1x5

Plasmid

#63599

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry pBSDONR P4r-P2

Plasmid

#121528

Purpose

For making C-terminal mCherry fusions using multisite Gateway cloning

Depositor

Roger Innes

Article

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Insert

mCherry

Expression

Bacterial

Available Since

Aug. 17, 2019

Availability

Academic Institutions and Nonprofits only

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GST HP1 gamma deltaC

Plasmid

#24077

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 gamma (CBX3 Human)

Tags

GST

Expression

Bacterial

Mutation

Missing the C-terminal 25 amino acids

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
KIF1C-FHA

Plasmid

#25549

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

KIF1C-FHA (KIF1C Human)

Tags

His

Expression

Bacterial

Mutation

FHA domain only

Available Since

Aug. 25, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pFLIP30

Plasmid

#65499

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-mCer-cMyc

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFLIP32

Plasmid

#65500

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFLIP39

Plasmid

#65507

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMyc

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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p35SZ

Plasmid

#51513

Purpose

pMDC32 with Zif268:FokI followed by approximately 3kb of filler sequence

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
3kb filler sequence

Use

Plant expression t-dna

Tags

NLS

Promoter

2x35S

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pLSLZ.D

Plasmid

#51496

Purpose

pLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmid

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

SV40 NLS

Promoter

virion-sense LIR and 2x35S

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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U371-GFP ILtoEK

Plasmid

#24472

Depositor

Ron Vale

Article

Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

Insert

Unc104 (unc-104 Nematode)

Tags

GFP

Expression

Bacterial

Mutation

Contains only the kinesin's motor head fused…

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
pLRK36

Plasmid

#253105

Purpose

Expresses SpCas9-P2A-RUBY under ZmUbi1 promoter and two gRNAs for targeting NAC21/22 genes in wheat

Depositor

Ksenia Krasileva

Article

Kumar et al bioRxiv 2026.02.10.705172

Insert

SpCas9-P2A-RUBY

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi1

Available Since

May 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLRK57

Plasmid

#253106

Purpose

Expresses SpCas9-P2A-RUBY under ZmUbi1 promoter and two gRNAs for targeting DWF4 genes in wheat

Depositor

Ksenia Krasileva

Article

Kumar et al bioRxiv 2026.02.10.705172

Insert

SpCas9-P2A-RUBY

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi1

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pLRK35

Plasmid

#253104

Purpose

Expresses SpCas9-P2A-RUBY under ZmUbi1 promoter and two gRNAs for targeting DND2 genes in wheat

Depositor

Ksenia Krasileva

Article

Kumar et al bioRxiv 2026.02.10.705172

Insert

SpCas9-P2A-RUBY

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi1

Available Since

April 29, 2026

Availability

Academic Institutions and Nonprofits only

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