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EGFP-Rab4a-7

Plasmid

#56440

Purpose

Localization: Ras GTPase, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Insert

Rab4a (RAB4A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pT2Apuro-TRE-synPCB2.0

Plasmid

#139481

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7

Use

Tol2 transposon donor vector

Expression

Mammalian

Promoter

Tet responsive element

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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TALEN-CCR5L18-CtermQ3

Plasmid

#51440

Purpose

Expresses TALEN targeting left CCR5A site (L18) with Q3 C-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Left (L18) Q3 C-term

Use

TALEN

Tags

3xFLAG and Fok1 EL variant

Expression

Mammalian

Mutation

K788Q, R792Q and R801Q

Promoter

CMV

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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TALEN-CCR5R18-CtermQ3

Plasmid

#51441

Purpose

Expresses TALEN targeting right CCR5A site (R18) with Q3 C-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Right (R18) Q3 C-term

Use

TALEN

Tags

3xFLAG and Fok1 KK variant

Expression

Mammalian

Mutation

K788Q, R792Q and R801Q

Promoter

CMV

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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His6-tev-G(TTR F87E)

Plasmid

#242846

Purpose

Bacterial expression of TTR

Depositor

Peter Wright

Article

Ritsch et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

TTR (TTR Human)

Tags

His6tev-G

Expression

Bacterial

Mutation

M0G, N-terminal methionine mutated to glycine, F8…

Promoter

T7

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pEasyG3_nat

Plasmid

#184918

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG3_mic

Plasmid

#184920

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Kif5a-Myc

Plasmid

#186613

Purpose

Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform A fused to Myc tag

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Heavy Chain isoform 5A (KIF5A Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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YFP-CD82

Plasmid

#1819

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

CD82 (CD82 Human)

Tags

YFP

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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Stat3 S727A pRc/CMV

Plasmid

#8708

Depositor

Jim Darnell

Article

Wen et al Nucleic Acids Res 1997 Jun 1;25(11):2062

Insert

Stat3 S727A (Stat3 Mouse)

Expression

Mammalian

Mutation

S727A (exhibits o serine phosphorylation either c…

Available Since

Aug. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pEYFP-hDF

Plasmid

#72584

Purpose

human dysferlin full length tagged with YFP

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin (DYSF Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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MTK1_008

Plasmid

#123671

Purpose

Encodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro

Plasmid

#211687

Purpose

U6-sgRNA-EFS-Cas9-P2A-Puro

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and guide RNA recipient vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-HA-NBP1 (P#1261)

Plasmid

#14586

Depositor

Matija Peterlin

Article

Lu et al Immunity. 1998 May . 8(5):647-56.

Insert

ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H Human)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
MTK1_009

Plasmid

#123672

Purpose

Encodes ConL1 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L1-2xHS4

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4 mIL-17 2kb promoter + CNS-5

Plasmid

#20128

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Available Since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only

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MTK1_013

Plasmid

#123676

Purpose

Encodes ConL5 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L5-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3_V5-APEX2-HMOX1

Plasmid

#85824

Purpose

Expressed N-term tagged APEX2 on HMOX1 in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

Insert

V5-APEX2-HMOX1 (HMOX1 Human, Synthetic)

Tags

APEX2 and V5

Expression

Mammalian

Promoter

CMV-F

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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Cx26-msfGFP

Plasmid

#69016

Purpose

Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx26 (Gjb2 Rat)

Tags

V206K monomerized super folder GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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EBFP2-Cx30

Plasmid

#69022

Purpose

Expresses human Cx30 (Gjb6 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx30 (GJB6 Human)

Tags

EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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