We narrowed to 7,885 results for: Lif

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Jam3-Fc-His

Plasmid

#72080

Purpose

Expresses the extracellular region of the JAM-3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam3 (Jam3 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Lrrc4-AP-His

Plasmid

#71958

Purpose

Expresses the extracellular region of the LRRC4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Lrrc4 (Lrrc4 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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Jam3-AP-His

Plasmid

#71954

Purpose

Expresses the extracellular region of the JAM-3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam3 (Jam3 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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Jam4.1-AP-His

Plasmid

#71955

Purpose

Expresses the extracellular region of the JAM-4, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam4.1 (Igsf5 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Jam4.3-AP-His

Plasmid

#71956

Purpose

Expresses the extracellular region of the JAM-4, isoform 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam4.3 (Igsf5 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ATF4

Plasmid

#192911

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of ATF4

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

ATF4 (ATF4 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET-26b-Nb127D01-ALFA-His

Plasmid

#171568

Purpose

Bacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-ALFA-His (CXCR2 Human)

Tags

ALFA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

Plasmid

#196185

Purpose

Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His

Depositor

Jesse Zalatan

Article

Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

Inserts

GSK3B (GSK3B Human)
λPPase

Tags

GST, HA, His6, and MBP

Expression

Bacterial

Mutation

changed Serine 9 to Alanine

Promoter

Tac

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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AKAP1-EGFP-uTEV1

Plasmid

#171005

Purpose

HiLITR protease with AKAP1 N-terminal tmd targeting information (Mitochondria)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

AKAP1(tmd)-EGFP-uTEV1 (AKAP1 Synthetic)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CSF1R-V5/HIS

Plasmid

#201984

Purpose

expression of human CSF1R receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

CSF1R receptor tyrosine kinase (CSF1R Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-FOXL2

Plasmid

#192914

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of FOXL2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

FOXL2 (FOXL2 Human)

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.27_eSOX17

Plasmid

#195498

Purpose

Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the extended endoderm distal enhancer of human SOX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

Full distal endoderm enhancer region of SOX17 (SOX17 Human)

Tags

minP_luc2-hPEST

Expression

Mammalian

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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PB53x EF1-Dendra2-RPB1Amr

Plasmid

#81228

Purpose

expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system

Depositor

Timothée Lionnet

Article

Cho et al Elife. 2016 May 3;5. pii: e13617. doi: 1

Insert

Dendra2 - Rpb1 (alpha amanitin resistant) (POLR2A Human)

Tags

Dendra2

Expression

Mammalian

Mutation

N792D alpha amanitin resistance mutation (Bartolo…

Promoter

EF1a

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

Plasmid

#188899

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VEGFR3-V5/HIS

Plasmid

#205618

Purpose

Expression of human VEGFR3 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

VEGFR3 receptor tyrosine kinase (FLT4 Human)

Tags

V5-His

Expression

Mammalian

Mutation

T28M, S455R- Please see depositor comment

Promoter

CMV

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ERBB3-V5/HIS

Plasmid

#201104

Purpose

expression of human ERBB3 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human ERBB3 receptor tyrosine kinase, full length, wildtype (ERBB3 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-NR4A1-P2A-mCherry

Plasmid

#203859

Purpose

Tet-inducible lentiviral plasmid expressing NR4A1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

NR4A1 (NR4A1 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FGFR2c-V5/HIS

Plasmid

#201107

Purpose

expression of human FGFR2 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FGFR2 receptor tyrosine kinase, variant c, full length, wildtype (FGFR2 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-ZEB2-P2A-mCherry

Plasmid

#203857

Purpose

Tet-inducible lentiviral plasmid expressing ZEB2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

ZEB2 (ZEB2 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-DDR1-V5/HIS

Plasmid

#201100

Purpose

expression of human DDR1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human DDR1 receptor tyrosine kinase, full length, wildtype (DDR1 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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