Addgene

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pCML 221

Plasmid

#98587

Purpose

pMS2-CYFP. Expresses MS2 protein-CYFP fusion in E. coli for TriFC assay (kanR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Insert

MS2

Use

Tags

Expression

Mutation

Promoter

pLacO

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAM-PAT-ProUBQ10 GW

Plasmid

#79751

Purpose

binary DEST vector for plant expression

Depositor

Arp Schnittger, Nico Dissmeyer

Article

Faden et al Nat Commun. 2016 Jul 22;7:12202. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

ProUBQ10

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pILGFPJ1

Plasmid

#83561

Purpose

Express yEGFP-ERG9 ER signal peptide under the control of TEF1 promoter

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

TEF1 promoter-yEGFP-ERG9 ER signal peptide

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR4 MOV10 D645N

Plasmid

#51714

Purpose

plasmid contains the coding sequence of MOV10 D645N

Depositor

Markus Landthaler

Article

Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

Insert

MOV10 D645N (MOV10 Human)

Use

Entry vector

Tags

Expression

Mutation

D645N

Promoter

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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Danio rerio cryaa

Plasmid

#46166

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin (cryaa Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only

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Cyprinodon variegatus cryaa

Plasmid

#47664

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin (cryaa Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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Syntaxin-4 M2 (Y115A, Y148A, Y251A)

Plasmid

#44030

Depositor

Thomas Weimbs

Article

Reales et al PLoS One. 2011;6(6):e21181. doi: 10.1371

Insert

Syntaxin-4

Use

Tags

2xMyc and His

Expression

Mammalian

Mutation

Y115A Y148A Y251A

Promoter

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only
pET21-MRPS11

Plasmid

#31343

Depositor

Linda Spremulli

Article

Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

Insert

MRPS11 Mature form (MRPS11 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Mature for after removal of the predicted mitocho…

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-KG/Net1A

Plasmid

#28261

Depositor

Jeffrey Frost

Article

Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

Insert

Net1A (Net1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 11, 2011

Availability

Academic Institutions and Nonprofits only
AIRAP 5: AIRAP(1-50).GST,V1.pcDNA3

Plasmid

#21795

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

AIRAP (Zfand2a Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

amino acids 1-50

Promoter

Available since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-GW1D1aMut(W>A)

Plasmid

#21522

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contain aa254-503, mutate every tryptophan (total…

Promoter

Available since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
pHWBF06EC

Plasmid

#130827

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Gateway entry vector

Tags

mCherry-FLAG-HA

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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SUN2delCC

Plasmid

#130836

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Tags

mCherry-FLAG-HA

Expression

Plant

Mutation

Deletion of coiled coil domain

Promoter

CaMV 35S

Available since

Availability

Academic Institutions and Nonprofits only

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SUN2delSUNec

Plasmid

#130847

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Gateway entry vector

Tags

mCherry-FLAG-HA

Expression

Mutation

Deletion of SUN domain

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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SUN2delCCec

Plasmid

#130848

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Gateway entry vector

Tags

mCherry-FLAG-HA

Expression

Mutation

Deletion of coiled coil domain

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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dCas9-10xSunTag-P2A-tagBFP

Plasmid

#174141

Purpose

Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

Depositor

Albert Jeltsch

Article

Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

Insert

dCas9, 10xSunTag, tagBFP

Use

Tags

HA and tagBFP

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-H2BC11

Plasmid

#207755

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMK381 (AAVS1 CMV-OsTIR1F74G)

Plasmid

#140536

Purpose

AAVS1 CMV-OsTIR1(F74G)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CMV-OsTIR1(F74G)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-mTrim21

Plasmid

#105516

Purpose

Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag Yap1

Plasmid

#18881

Depositor

Yosef Shaul

Article

Levy et al Mol Cell. 2008 Feb 15. 29(3):350-61.

Insert

Yap1 (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2008

Availability

Academic Institutions and Nonprofits only

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