We narrowed to 5,882 results for: pcDNA 3.1

1. pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

   Plasmid

   #169597

   Purpose

   Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   dead S.pyogenes Cas9 - VP64 fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVH19-Tier1-PhCMV-p300core

   Plasmid

   #169572

   Purpose

   Tier-1 vector encoding PhCMV-driven histone acetyltransferase p300 core domain expression (PhCMV-p300core-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven P300core (EP300 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS2304-Tier1-Tactb-PPGK-NLuc_TB

   Plasmid

   #169549

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   Tactb-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS1046-Tier1-PhCMV-4xCox8-mRuby2

   Plasmid

   #169537

   Purpose

   Tier-1 vector encoding PhCMV-driven mRuby2 localized to the mitochondrial matrix via a four-fold Cox8 localization signal (PhCMV-4xCox8-mRuby2-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven miochondria-localized monomeric red fluorescent protein mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTS2380-Tier1-PhCMV-TM

   Plasmid

   #169485

   Purpose

   Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) (PhCMV-TM-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven Junctophilin 2 transmembrane domain (JPH2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGlomyc-Ovol1

   Plasmid

   #172871

   Purpose

   Mammalian expression vector with myc-tagged mouse Ovol1 coding sequence

   Depositor

   Renee van Amerongen

   Article

   Mourao et al bioRxiv 2021.06.22.449461

   Insert

   Ovol1 (Ovol1 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGAL4-DBD-OLIG2wt-IDR

   Plasmid

   #145250

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and OLIG2wt IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   OLIG2wt-IDR (OLIG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGAL4-DBD-MNX1wt-IDR

   Plasmid

   #145248

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and MNX1wt IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   MNX1wt-IDR (MNX1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EGFP-Mm_Plk5(D168A)

   Plasmid

   #136318

   Purpose

   Mammalian expression of mouse Plk5-KD (D168A) with N-terminal EGFP

   Depositor

   Marcos Malumbres, Guillermo de Cárcer

   Article

   de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

   Insert

   PLK5 (Plk5 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D168A

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-Hu_Plk5(W207F)

    Plasmid

    #136320

    Purpose

    Mammalian expression of human Plk5 W207F with N-terminal EGFP

    Depositor

    Marcos Malumbres, Guillermo de Cárcer

    Article

    de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

    Insert

    PLK5 (PLK5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W207F

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Antares-SEPLuc C1A4E

    Plasmid

    #141088

    Purpose

    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-SEP-C1A4E
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Please see depositor comments

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Opto-GPR148

    Plasmid

    #106048

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR148; Opto-GPR148; E2)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR148 (GPR148 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Opto-GPR78

    Plasmid

    #106033

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR78; Opto-GPR78; C11)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR78 (GPR78 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Opto-GPR42

    Plasmid

    #106022

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR42; Opto-GPR42; B12)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR42 (GPR42 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Opto-GPR32

    Plasmid

    #106015

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR32; Opto-GPR32; B5)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR32 (GPR32 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. hHSF4b K294R

    Plasmid

    #118357

    Purpose

    This plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hHSF4b (HSF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K294R

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. gamma-tubulin (334-451)

    Plasmid

    #104436

    Purpose

    expressing a fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS

    Depositor

    Maria Alvarado-Kristensson

    Article

    Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

    Insert

    gamma-tubulin fragment 334 to 451 (TUBG1 Human)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    deleted a.a. 1 to 333

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FLAG-Atoh1 S334A

    Plasmid

    #96915

    Purpose

    Murine Atoh1 with S334A mutation

    Depositor

    Albert Edge

    Article

    Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Serine 334 changed to Alanine

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FLAG-Atoh1 S309A

    Plasmid

    #96916

    Purpose

    Murine Atoh1 with S309A mutation

    Depositor

    Albert Edge

    Article

    Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Serine 309 changed to Alanine

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FLAG-Atoh1 S325A

    Plasmid

    #96926

    Purpose

    Murine Atoh1 with S325A mutation

    Depositor

    Albert Edge

    Article

    Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Serine 325 changed to Alanine

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FLAG-Atoh1 S328A

    Plasmid

    #96927

    Purpose

    Murine Atoh1 with S328A mutation

    Depositor

    Albert Edge

    Article

    Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Serine 328 changed to Alanine

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FLAG-Atoh1 S331A

    Plasmid

    #96928

    Purpose

    Murine Atoh1 with S331A mutation

    Depositor

    Albert Edge

    Article

    Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Serine 331 changed to Alanine

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FLAG-Atoh1 S339A

    Plasmid

    #96929

    Purpose

    Murine Atoh1 with S339A mutation

    Depositor

    Albert Edge

    Article

    Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Serine 339 changed to Alanine

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CC1

    Plasmid

    #63713

    Purpose

    Wild-type voltage sensing domain from Ciona voltage sensing phosphatase fused to super ecliptic A227D with restriction site flanking each transmembrane domain

    Depositor

    Bradley Baker

    Article

    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

    Insert

    CC1
    

    Use

    Tags

    Super ecliptic pHlorin A227D

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pmyc-GFP-TNRC6B-(997-1084)-NES-mut

    Plasmid

    #42025

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6B (TNRC6B Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa997-1084, L1009A, F1013A, M1016A, L1018A

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. myr-Abr

    Plasmid

    #38174

    Depositor

    Nora Heisterkamp

    Article

    Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

    Insert

    ABR transcript variant 2 (ABR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    addition of a Src myristoylation sequence (see co…

    Promoter

    Available since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

27. CMV-sgE1TSS-short-3'box

    Plasmid

    #92165

    Purpose

    sgRNA expression vector for Evx1as RNA tethering assay

    Depositor

    Xiaohua Shen

    Article

    Luo et al Cell Stem Cell. 2016 May 5;18(5):637-52.

    Insert

    Evx1as short isoform
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CaMKAR

    Plasmid

    #205315

    Purpose

    Fluorescent Reporter for CaMKII Activity

    Depositor

    Elizabeth Luczak, Mark Anderson

    Article

    Reyes Gaido et al Sci Transl Med. 2023 Jun 21;15(701):eabq

    Insert

    CaMKAR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pmGFP-ADAR1-p150

    Plasmid

    #117927

    Purpose

    ADAR1-p150 expression plasmid.

    Depositor

    Kumiko Ui-Tei

    Article

    Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

    Insert

    ADAR1-p150 (ADAR Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pmGFP-ADAR1-p110

    Plasmid

    #117928

    Purpose

    ADAR1-p110 expression plasmid.

    Depositor

    Kumiko Ui-Tei

    Article

    Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

    Insert

    ADAR1-p110 (ADAR Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge