We narrowed to 8,419 results for: BLI;

1. pT7CFE1_EVA71_VP12A

   Plasmid

   #212977

   Purpose

   Encodes EV-A71 4643 VP12A optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   Enterovirus A71 4643 VP12A
   

   Use

   Mammalian cell free protein expression

   Tags

   HA tag

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7CFE1_EVD68_VP12A

   Plasmid

   #212984

   Purpose

   Encodes EV-D68 MO/14/18947 VP12A optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 VP12A
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7CFE1_EVD68_3ABC

   Plasmid

   #212979

   Purpose

   ncodes EV-D68 MO/14/18947 3ABC optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 3ABC
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pT7CFE1_EVD68_3ABCD

   Plasmid

   #212981

   Purpose

   Encodes EV-D68 MO/14/18947 3ABCD optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 3ABCD
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC-ODN

   Plasmid

   #208642

   Purpose

   Empty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   Long single strand dna production

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR3cDual-Wnk

   Plasmid

   #203976

   Purpose

   Enrty clone for Gateway cloning

   Depositor

   Andreas Jenny

   Insert

   Wnk (Wnk Fly)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. DV.7.1

   Plasmid

   #208584

   Purpose

   Mammalian expression vector for expressing DV.7.1 SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation

   Depositor

   Ben Murrell

   Insert

   SARS-CoV-2 variant DV.7.1 spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA Display H3_A/Hong Kong/1/1968 Part 4-DO

   Plasmid

   #206518

   Purpose

   CcdB Dropout vector for part 4 of Hemagglutinin surface expression in mammalian cells

   Depositor

   IIya Finkelstein

   Type

   Empty backbone

   Use

   Tags

   3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA Display H3_A/Hong Kong/1/1968 Part 1-DO

   Plasmid

   #206515

   Purpose

   CcdB Dropout vector for part 1 of Hemagglutinin surface expression in mammalian cells

   Depositor

   IIya Finkelstein

   Type

   Empty backbone

   Use

   Tags

   3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG416GAL-Bat-SL-CoVZC45_PLP

    Plasmid

    #201939

    Purpose

    Expresses Bat-SL-CoVZC45 papain-like protease from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Insert

    Bat-SL-CoVZC45 PLP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG416GAL10-Bat-SL-CoVZC45_3CL

    Plasmid

    #201944

    Purpose

    Expresses Bat-SL-CoVZC45 3CL protease from a GAL10 promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Insert

    Bat-SL-CoVZC45 3CL protease
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG416GAL10-DUGV_OTU

    Plasmid

    #203463

    Purpose

    Expresses DUGV OTU domain of L protein from a GAL10 promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Insert

    DUGV OTU domain of L protein (DUGVsLgp1 )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG416GAL-3x-uC-HCoV-229E_3CL

    Plasmid

    #201937

    Purpose

    Expresses HCoV-229E 3CL protease from a GAL-3x promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Insert

    HCoV-229E 3CL protease
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGMC00024 (aka pCW-Cas9n-Blast)

    Plasmid

    #195315

    Purpose

    Dox inducible lentiviral Cas9 nickase

    Depositor

    Raj Chari

    Insert

    Cas9-nickase
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A nickase

    Promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEXP1-DEST-Noa1-HIS

    Plasmid

    #191972

    Purpose

    Destination vector containing HIS-tagged NOA1 coding sequence.

    Depositor

    Luca Scorrano

    Insert

    Noa1-HIS (NOA1 Human)
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-PARL-D319N-FLAG C-t

    Plasmid

    #191957

    Purpose

    Expression of Presenilin Associated Rhomboid Like (PARL) D319N mutant

    Depositor

    Luca Scorrano

    Insert

    PARL D319N (PARL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D319N

    Promoter

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. R6K_tac_dGFPmut3_ChInt

    Plasmid

    #187388

    Purpose

    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible destabilized GFPmut3 (containing a AAV C-terminal tail)

    Depositor

    Roi Avraham

    Insert

    dGFPmut3_AAV
    

    Use

    Tags

    AAV

    Expression

    Bacterial

    Mutation

    C-terminal AAV tail

    Promoter

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. KBB96

    Plasmid

    #185113

    Purpose

    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated so only one FXFG repeat remains

    Depositor

    Ken Belanger

    Insert

    NUP1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Last FXFG domain of Nup1

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. KBB109

    Plasmid

    #185066

    Purpose

    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated after NsiI restriction site

    Depositor

    Ken Belanger

    Insert

    NUP1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Nup1 C-terminal region, truncated at NsiI restric…

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLZRS-TrkC-mCherry

    Plasmid

    #182949

    Purpose

    To express the rat TrkC fused in the C-terminus with mCherry

    Depositor

    Pantelis Tsoulfas

    Insert

    TRk-C-mCherry (Ntrk3 Synthetic, Rat)
    

    Use

    Retroviral and Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    MMLV

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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