We narrowed to 21,800 results for: Sis

1. lenti-SAM-hygro

   Plasmid

   #138955

   Purpose

   Lentiviral vector for co-expressing spCas9, dRNA-MS2, p65 and HSF1 fused to MS2 binding protein, and hygromycin resistance gene

   Depositor

   Nadya Dimitrova

   Article

   Olivero et al Mol Cell. 2020 Feb 20;77(4):761-774.e8.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 AND EF1a

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7C-DNAJB4

   Plasmid

   #177656

   Purpose

   Recombinant protein synthesis of human DNAJB4

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   DNAJB4 (DNAJB4 Human)
   

   Use

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUB-nHyPer2 (NLS:HyPer2:NLS)

   Plasmid

   #84730

   Purpose

   Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Insert

   HyPer2 targeted to nucleus
   

   Use

   Tags

   NLS (nuclear localisation signal and NLS (nuclear…

   Expression

   Plant

   Mutation

   Promoter

   ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Htr5b(3.7)-EGFP-sv40

   Plasmid

   #184411

   Purpose

   AAV expression of EGFP from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.

   Depositor

   Marylka Yoe Uusisaari

   Article

   Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

   Insert

   Mouse Htr5b gene promoter-EGFP (Htr5b Mouse)
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Htr5b gene

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pS848∙CsR

   Plasmid

   #199240

   Purpose

   Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   gRNA scaffold - Gm resistance cassette - incP oriT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PEM7

   Available since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT/Caggs-NRAS G12V-IRES-ASK1

   Plasmid

   #221074

   Purpose

   Expresses NRAS G12V and ASK1 in mammalian cells

   Depositor

   Hidenori Ichijo

   Article

   Odawara et al Commun Biol. 2024 Jun 5;7(1):691. doi: 1

   Inserts

   NRAS (NRAS Human)
   ASK1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUBQ10:AtFLS2

   Plasmid

   #202186

   Purpose

   Express Arabidopsis thaliana FLS2 gene under UBQ10 promoter

   Depositor

   Gitta Coaker

   Article

   Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

   Insert

   Flagellin sensing 2 (FLS2 Mustard Weed)
   

   Use

   Tags

   HA

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUB-nSypHer (NLS:sypHer:NLS)

   Plasmid

   #84731

   Purpose

   Expresses sypHer in plant cells-biosensor for pH targeted to Nuclei

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Insert

   nSypHer
   

   Use

   Tags

   NLS (nuclear localisation signal and NLS (nuclear…

   Expression

   Plant

   Mutation

   Promoter

   ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRE-Tight-FLAG-CYLD-WT

   Plasmid

   #60030

   Purpose

   WT CYLD under control of Tet promoter with N-terminal FLAG tag

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Nov. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

10. cfmTurquoise-N

    Plasmid

    #139284

    Purpose

    Cys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.

    Depositor

    Ikuo Wada

    Article

    Omae et al Transfusion. 2019 Jul;59(7):2429-2435. d

    Insert

    cfmTurquoise
    

    Use

    Tags

    Multi-cloning sites

    Expression

    Mammalian

    Mutation

    Cys48Ser and Cys70/Met

    Promoter

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGMEH-T38M-750-luxAB-DAS+4

    Plasmid

    #49520

    Purpose

    Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomally

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    T38M-P750-luxAB-DAS+4
    

    Use

    Tags

    DAS+4 (AANDENYSENYADAS)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. SEP-Nanoluc-cyOFP1

    Plasmid

    #141082

    Purpose

    prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    SEP-Nanoluc-cyOFP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of amino acids DISGG from positions 673 …

    Promoter

    EF1α

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBtPIPLC His

    Plasmid

    #173808

    Purpose

    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

    Depositor

    Anne Gershenson, Mary F Roberts

    Article

    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

    Insert

    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCold I-Hero7

    Plasmid

    #187926

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero7

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero7 (SERF2 Human)
    

    Use

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCold I-Hero45

    Plasmid

    #187931

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero45

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero45 (SERBP1 Human)
    

    Use

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AID-BFP-loxP_myo2_neoR

    Plasmid

    #194054

    Purpose

    Dual-selection cassette plasmid for knocking-in AID-BFP into the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Use

    Tags

    bfp

    Expression

    Worm

    Mutation

    Promoter

    None

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCA-T-intron(Neo)-G(LNL)

    Plasmid

    #36908

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (i.e., ATG, start codon as a first exon)
    GFP
    beta-globin intron
    Neo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides before nucleotide 275 and ins…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. HBT-CPK32-Flag

    Plasmid

    #107432

    Purpose

    For Arabidopsis mesophyll protoplast transient expression assay

    Depositor

    Jen Sheen

    Article

    Liu et al Nature. 2017 May 18;545(7654):311-316. d

    Insert

    CPK32 (CPK32 Mustard Weed)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEM-PH-citrine

    Plasmid

    #131406

    Purpose

    Purpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrine

    Depositor

    Lars Hufnagel

    Article

    Guignard et al Science. 2020 Jul 10;369(6500). pii: 369

    Insert

    PH
    

    Use

    In vitro transcription

    Tags

    citrine yellow fluorescent protein

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS

    Plasmid

    #127365

    Purpose

    Binary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Plant

    Mutation

    R118G

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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