We narrowed to 6,961 results for: RAP

1. pTriEx-mVenus-Zdk1-RhoA Q63L

   Plasmid

   #81046

   Purpose

   mammalian expression of Zdk1 tagged RhoA Q63L

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Inserts

   Zdk1
   RhoA Q63L (RHOA Human)
   

   Use

   Tags

   mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiDP1.0

   Plasmid

   #171094

   Purpose

   lentiviral construct (3rd generation) for the expression of cell cycle-independent OsTIR1 fused with mEmerald and miniAID-fused mCherry2

   Depositor

   Dario Palmieri

   Article

   Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

   Insert

   OsTIR1-mEmerald-P2A-miniAID-mCherry2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLXSN-Axl DN

   Plasmid

   #65223

   Purpose

   Expression of dominant negative Axl in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Zhang et al Cancer Res. 2008 Mar 15;68(6):1905-15. d

   Insert

   AXL DN (AXL Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   deleted cytoplasmic domain

   Promoter

   LTR

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

4. ABE8.17-d

   Plasmid

   #136299

   Purpose

   expresses ABE8.17-d in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.20-d
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-G88P3-HA-hM3Dq

   Plasmid

   #213972

   Purpose

   AAV vector with G88P3 promoter that can drive robust gene expression in MSNs

   Depositor

   Zhonghua Lu

   Article

   Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

   Insert

   hM3D (Gq)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   G88P3

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28b-BE3∆UGI

   Plasmid

   #96943

   Purpose

   The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).

   Depositor

   Daesik Kim

   Article

   Kim et al Nat Biotechnol. 2017 May;35(5):475-480.

   Insert

   BE3∆UGI
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe puro-HA-PIK3CA(H1047R;I848V)

   Plasmid

   #33253

   Depositor

   Thomas Roberts

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   Hemagglutinin (HA)

   Expression

   Mammalian

   Mutation

   changed histidine 1047 to arginine and isoleucine…

   Promoter

   SV40

   Available since

   Jan. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pJW1838

   Plasmid

   #154324

   Purpose

   sgRNA Destination Vector for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   SapTrap sgRNA (F+E) vector, K09B11.2 U6 promoter and 3'UTR
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   F+E mutations in sgRNA

   Promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTriEx-mCherry-Zdk1-RhoA Q63L

   Plasmid

   #81058

   Purpose

   mammalian expression of Zdk1 tagged RhoA Q63L

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Inserts

   Zdk1
   RhoA Q63L (RHOA Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTriEx-mVenus-Zdk1-Rac1 Q61L

    Plasmid

    #81137

    Purpose

    mammalian expression of Zdk1 tagged Rac1 Q61L

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Inserts

    Zdk1
    Rac1 (RHOA Human)
    

    Use

    Tags

    mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q61L

    Promoter

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-CaMKII-pAceR-Kv2.1PR

    Plasmid

    #195531

    Purpose

    Red fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    pAceR-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    VARNAM 78E, 81D, 92N; SI linker

    Promoter

    CaMKII

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9-Puro HLAG-2A-sgRNA

    Plasmid

    #182551

    Purpose

    Cas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G gene

    Depositor

    Santiago Miriuka

    Article

    Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

    Insert

    hSpCas9-2A-Puro-HLAG-2A-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET48 AtGUN1-SMR1

    Plasmid

    #136361

    Purpose

    Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 709 to 918)

    Depositor

    Tatsuru Masuda

    Article

    Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

    Insert

    cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 709 to 918 (GUN1 Mustard Weed)
    

    Use

    Tags

    TRX-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET48 AtGUN1-SMR2

    Plasmid

    #136362

    Purpose

    Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 781 to 918)

    Depositor

    Tatsuru Masuda

    Article

    Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

    Insert

    cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 781 to 918 (GUN1 Mustard Weed)
    

    Use

    Tags

    TRX-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET48 AtGUN1-PPR1

    Plasmid

    #136359

    Purpose

    Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 708)

    Depositor

    Tatsuru Masuda

    Article

    Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

    Insert

    cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 708 (GUN1 Mustard Weed)
    

    Use

    Tags

    TRX-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET48 AtGUN1-PPR2

    Plasmid

    #136360

    Purpose

    Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 619)

    Depositor

    Tatsuru Masuda

    Article

    Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

    Insert

    cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 619 (GUN1 Mustard Weed)
    

    Use

    Tags

    TRX-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDH-3xFLAG-hTCAB1-puroR

    Plasmid

    #167459

    Purpose

    3rd-gen lentiviral expression of FLAG-TCAB1 (WDR79) in mammalian cells

    Depositor

    Steven Artandi

    Article

    Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

    Insert

    TCAB1 (WRAP53 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. JpExpress404 PTEN

    Plasmid

    #49420

    Purpose

    Bacterial Expression Vector

    Depositor

    Ramon Parsons

    Article

    Hopkins et al Science. 2013 Jul 26;341(6144):399-402.

    Insert

    PTEN (PTEN )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

19. AA290

    Plasmid

    #215949

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD55_v4; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AA286

    Plasmid

    #215948

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD47_v6; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 2xFKBP-smFP(myc)-KDEL

    Plasmid

    #173021

    Purpose

    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    2xFKBP-smFP(myc)-KDEL
    

    Use

    Tags

    FKBP and smFP(myc) non-fluorescent

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-pmEMBer

    Plasmid

    #174441

    Purpose

    Plasma membrane-targeted ERK monobody binder for local inhibition of ERK activity in live cells.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    pmEMBer
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLPC-MYC.Mm.KAT5

    Plasmid

    #184652

    Purpose

    Retroviral expression of mouse Kat5

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    Kat5 (Kat5 Mouse)
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pENTR221-GLI1

    Plasmid

    #79497

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human GLI1 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    GLI1 (GLI1 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. VanGlow-GL-DSCP

    Plasmid

    #83338

    Purpose

    for Gateway cloning of enhancer elements upstream of the DSCP and GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

    Depositor

    Cheng-Yu Lee

    Article

    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    GFP::Luciferase(nls)

    Expression

    Insect

    Mutation

    Promoter

    Drosophila Synthetic Core Promoter

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENTR221-FOS

    Plasmid

    #79511

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human FOS CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    FOS (FOS Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRSETC-RdgBbeta-splice variant 1

    Plasmid

    #58276

    Purpose

    bacterial expression of human RdgBbeta splice variant 1

    Depositor

    Shamshad Cockcroft

    Article

    Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

    Insert

    RdgBbeta (PITPNC1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pMX-IY LC3C

    Plasmid

    #89292

    Purpose

    For expression of the mammalian Atg8 protein LC3C without a tag

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    LC3C (MAP1LC3C Human)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSF11-wmiRFP2-T2A-HO1

    Plasmid

    #179485

    Purpose

    Expresses miRFP2 and HO1in worm cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    wmIRFP2-T2A-HO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NO

    Promoter

    tag-168 promoter

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TOPO UBC

    Plasmid

    #68456

    Purpose

    TOPO construct expressing UBC for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Ubiquitin C Promoter (UBC Human)
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge