We narrowed to 4,857 results for: Mela

1. pHD-T2A-split-intein-Gal4[21-881]-C-int

   Plasmid

   #199204

   Purpose

   To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[21-881]-C-int
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti6/V5-DEST-ASF1B

   Plasmid

   #31205

   Depositor

   Lynda Chin

   Article

   Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

   Insert

   ASF1B (ASF1B Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

3. 20XUAS IVS ChrimsonR mCherry_tr

   Plasmid

   #111546

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR_mCherry_trafficked
   

   Use

   Tags

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. VP-FKBP51

   Plasmid

   #89097

   Purpose

   mammalian expression of FKBP5 also referred to as FKBP51 with N-terminal VP16 activation domain

   Depositor

   Elizabeth Wilson

   Article

   Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

   Insert

   FKBP5 (FKBP5 Human)
   

   Use

   Tags

   FLAG and VP16

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. SiriusGFP

   Plasmid

   #123201

   Purpose

   We have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.

   Depositor

   Joseph Santos-Sacchi

   Article

   Zhong et al J Neurosci Methods. 2019 Feb 1;313:68-76

   Insert

   SiriusGFP
   

   Use

   Mouse Targeting

   Tags

   N/A

   Expression

   Bacterial and Mammalian

   Mutation

   eGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJC-repo1.9kb-TALE4-VP64-P10

   Plasmid

   #104612

   Purpose

   Expresses TALE4 under the control of a 1.9kb repo enhancer element

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   1.9kb repo enhancer element (repo Fly)
   

   Use

   TALEN

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available Since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-Y1175F-VEGFR2-mCherry

   Plasmid

   #139608

   Purpose

   Expresses a mutant (Y1175F) VEGFR2-mCherry fusion protein in mammalian cells

   Depositor

   Michael Welsh

   Article

   Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

   Insert

   KDR (KDR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBABR IRFP-Bcd-LEXY

   Plasmid

   #182595

   Purpose

   Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

   Depositor

   Jared Toettcher

   Article

   Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

   Insert

   Bcd (bcd Fly)
   

   Use

   Tags

   IRFP and LEXY

   Expression

   Insect

   Mutation

   Promoter

   maternal tubulin

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. His Puc (aa 1-393)

   Plasmid

   #52295

   Purpose

   Bacterial expression of His tagged Drosophila Puc fragment

   Depositor

   Brenda Schulman

   Article

   Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

   Insert

   Puc (puc Fly)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pCDF1-PTPRD-G446E

    Plasmid

    #25643

    Depositor

    Todd Waldman

    Article

    Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

    Insert

    Protein tyrosine phosphatase receptor-type delta (PTPRD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    tumor-derived mutation created by site-directed m…

    Promoter

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

11. 10XUAS IVS ChrimsonR mCherry_tr

    Plasmid

    #111547

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

    Depositor

    Vivek Jayaraman

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR_mCherry_trafficked
    

    Use

    Tags

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-Y951F-VEGFR2-mCherry

    Plasmid

    #139607

    Purpose

    Expresses a mutant (Y951F) VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL410-BRN2p

    Plasmid

    #110733

    Purpose

    Luciferase reporter for the human BRN2 promoter

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    BRN2 promoter luciferase (POU3F2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

    Plasmid

    #199197

    Purpose

    Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int-BlastR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAFW-Ago2

    Plasmid

    #50554

    Purpose

    Expression of FLAG tagged Ago2 in S2 cell

    Depositor

    Yukihide Tomari

    Article

    Iwasaki et al Mol Cell. 2010 Jul 30;39(2):292-9. doi:

    Insert

    Argonaute2 (AGO2 Fly)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available Since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. VP-ARF

    Plasmid

    #89122

    Purpose

    mammalian expression of p14-hARF linked to VP16 activation domain

    Depositor

    Elizabeth Wilson

    Article

    Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

    Insert

    p14-hARF (CDKN2A Human)
    

    Use

    Tags

    VP16

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

    Plasmid

    #113039

    Purpose

    AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCrysβ:ECFP-LexOP:mCherry-HrasG12V

    Plasmid

    #89887

    Purpose

    Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    mCherry-HRasG12V
    

    Use

    Unspecified

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-Y1214F-VEGFR2-mCherry

    Plasmid

    #139609

    Purpose

    Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSUPER-retro-puro-shOrai1

    Plasmid

    #89933

    Purpose

    Knockdown human Orai1 expression

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    Orai1 shRNA (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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