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pMaCTag-Z23

Plasmid

#120066

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Halo

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Halo

Use

Pcr template

Tags

Halo

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pETM6-HpaBC

Plasmid

#71260

Purpose

Expresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6

Depositor

Mattheos Koffas

Article

Jones et al Biotechnol Prog. 2015 Oct 21. doi: 10.10

Inserts

HpaB
HpaC

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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LentiGuide Cherry

Plasmid

#170510

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.

Depositor

Pavel Tolar

Article

Newman et al biorXiv 2021.03.02.433398

Inserts

S. pyogenes sgRNA cassette
mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a and hU6

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pRenilla

Plasmid

#118059

Purpose

GBPart - Renilla Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Renilla

Use

Luciferase and Synthetic Biology; Gbpart

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pJK02

Plasmid

#105133

Purpose

Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

Depositor

Joseph Sorg

Article

McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

Inserts

Upstream & Downstream pyrE deletion region
tetR
Cas9
gRNA

Use

E. coli - c. difficile shuttle vector

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_T5-ARG1

Plasmid

#106476

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Use

Synthetic Biology

Expression

Bacterial

Promoter

T5

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P11

Plasmid

#120022

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet-i

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet-i

Use

Pcr template

Tags

mScarlet-i

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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μ2-HA-WT

Plasmid

#32752

Depositor

Alexander Sorkin

Article

Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

Insert

μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5 HIS-Ub-K48R

Plasmid

#221368

Purpose

Expresses 6xHIS-tagged Ubiquitin containing K48R mutation in mammalian cells

Depositor

Aga Gambus

Article

Jones et al iScience. 2024 Jun 12;27(7):110260. doi:

Insert

Ubiquitin

Tags

6xHIS

Expression

Mammalian

Mutation

Changed Lysine 48 to Arginine

Promoter

CMV

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMRS-dCas9

Plasmid

#220193

Purpose

mini-Tn7 dCas9 expression vector

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9

Use

CRISPR

Expression

Bacterial

Mutation

Pseudomonas aeruginosa codon optimized

Promoter

promoterless

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEC2861 (pSpy1C-PgyrA_ffluc)

Plasmid

#218511

Purpose

replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Mutation

deletion of Plac_mrfp cassette

Promoter

PgyrA

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pVenus-C1-Cdc20 (1534)

Plasmid

#26058

Depositor

Jonathon Pines

Article

Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

Insert

Cdc20 (Cdc20 Human)

Tags

Venus

Expression

Mammalian

Available Since

Aug. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pBa.Kif1a 1-396.GFP

Plasmid

#45058

Depositor

Gary Banker, Marvin Bentley

Article

Jacobson et al Neuron. 2006 Mar 16;49(6):797-804.

Insert

Kif 1a (Kif1a Rat)

Tags

GFP

Expression

Mammalian

Mutation

motor domain aa 1-396

Promoter

Chicken Beta Actin

Available Since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only
pLV hUbC-dCas9 VP64-T2A-GFP

Plasmid

#53192

Purpose

Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 VP64 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x2

Plasmid

#58772

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET17_K560_GFP_His

Plasmid

#15219

Depositor

Ron Vale

Article

Woehlke et al Cell. 1997 Jul 25. 90(2):207-16.

Insert

Human neuronal kinesin heavy chain (KIF5B Human)

Tags

GFP and His

Expression

Bacterial

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pENTR5'-attP (pCM254)

Plasmid

#68311

Purpose

Gateway middle entry vector for full-length attP site for phiC31-mediated transgenesis.

Depositor

Christian Mosimann, Leonard Zon

Article

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Insert

attP

Use

Gateway entry vector

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pFAST-BAC-Cdc20wt (2193)

Plasmid

#39850

Depositor

Jonathon Pines

Article

Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

Insert

Cdc20 (CDC20 Human)

Use

Baculovirus expression

Tags

His

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
pMaCTag-P07

Plasmid

#124788

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGLuc

Plasmid

#135288

Purpose

GBPart - GLuc Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Gaussia Luciferase

Use

Luciferase and Synthetic Biology

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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