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We narrowed to 6,274 results for: nls

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Showing: 3541 - 3560 of 6274 results

  3. pcDNA3-N-HA-NEK7 K64M

    Plasmid
    #75143
    Purpose
    Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells
    Depositor
    Bruce Beutler
    Article
    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:
    Insert
    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Lys 64 mutated to Met; kinase inactive and F236L …
    Promoter
    CMV
    Available Since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    75143-plasmid-map-sequence-id-147264

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  7. pCMV-T7-PEmax-P2A-EGFP (LM1589)

    Plasmid
    #223136
    Purpose
    pCMV and pT7 Human expression plasmid for PEmax(nSpCas9(H840A)-M-MMLV_RT**)-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized PEmax-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and NLS(SV40)-NLS(cMyc)-P2A-EGFP
    Expression
    Mammalian
    Mutation
    M-MLV mutations from PE2 (Anzalone et al. Nature …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223136-plasmid-map-sequence-id-447644

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  18. pLex307-UCOE-SFFV-puroR-MCP-HSF1-VIRF2

    Plasmid
    #218557
    Purpose
    Encodes SFFV promoter-driven MCP-HSF1-VIRF2 with an N-terminal bipartite SV40 NLS, along with puromycin resistance
    Depositor
    Alejandro Chavez
    Insert
    MCP-HSF1-VIRF2
    Use
    Lentiviral
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Available Since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    218557-plasmid-map-sequence-id-430698

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  19. (829) SB Tet-ON dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid
    #195622
    Purpose
    Sleeping Beauty Tet-ON plasmid for induced expression of dCasphi-ZIM3 CRISPRi construct
    Depositor
    Tonia Rex
    Insert
    dCasphi
    Tags
    C-Myc (NLS), HA tag, and ZIM3
    Expression
    Mammalian
    Mutation
    P749A and P753A
    Promoter
    Tight TRE promoter; Tet-ON (doxycycline)
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    195622-plasmid-map-sequence-id-385115

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Showing: 3541 - 3560 of 6274 results