Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 15,661 results for: genome

Showing: 7081 - 7120 of 15661 results

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pSCL.039

Plasmid

#184973

Purpose

Test effect of extending a1/a2 on ADE2 editing rates in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1

Expression

Yeast

Mutation

ADE2 donor P272stop, a1/a2 length extended to 27 …

Promoter

Gal7

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265K

Plasmid

#173997

Purpose

Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A) for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

UNG-rAPOBEC1(R33A)-zCas9(D10A)

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

R33A in rAPOBEC1 and D10A in zCas9

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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PlmdCas12e VPR

Plasmid

#188516

Purpose

Expresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Tags

NLS-FLAG-VPR

Expression

Mammalian

Mutation

D659A/E756A/D922A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM-T Easy-Cas9-nls-CDS1

Plasmid

#160231

Purpose

Cas9-nuclear localization, level 0 of MoClo Golden Gate position CDS1

Depositor

Matias Kirst

Article

Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

Insert

Cas9-nuclear localization signal gene sequence, Golden Gate MoClo position CDS1

Use

Level 0 of moclo golden gate position cds1

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQDKN0760

Plasmid

#185629

Purpose

Modified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T (BsaI-domesticated) which targets phytoene desaturase (PDS) of Nicotiana benthamiana

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0751

Plasmid

#185628

Purpose

Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using BsaI

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

lacZ

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0001

Plasmid

#185625

Purpose

MoClo Level 1, position 2 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

SpCas9

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pTW037

Plasmid

#185688

Purpose

T-DNA for creating transgenic plants expressing Cas9 and Drm1b gRNAs

Depositor

Feng Zhang

Article

Weiss et al Plant J. 2020 Nov;104(3):828-838. doi: 1

Insert

Cas9, Drm1b gRNA

Use

CRISPR

Expression

Plant

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD2KN0899

Plasmid

#177092

Purpose

MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrSTR, CrSLS, CrLAMT, CrTDC, and Cr7-DLH driven by 4x opaatB

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

CrSTR, CrSLS, CrLAMT, CrTDC, and Cr7-DLH

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD2KN0895

Plasmid

#177091

Purpose

MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrSTR, Cr7-DLH, CrLAMT, CrTDC, and CrSLS driven by 4x opaatB

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

CrSTR, Cr7-DLH, CrLAMT, CrTDC, and CrSLS

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQDPKN0892

Plasmid

#177089

Purpose

MoClo Level P, position 1, plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, CrG8H, CrGOR, CrIO, and Cr7-DLGT driven by 4x opaatB

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

CrISY, NmMLPL, CrG8H, CrGOR, CrIO, and Cr7-DLGT

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0865

Plasmid

#177084

Purpose

MoClo Level 1, position 6, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geraniol 8-oxidase (CrG8H) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0802

Plasmid

#177083

Purpose

MoClo Level 1, position 1, transcriptional unit for transient expression of strictosidine synthase (CrSTR) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

strictosidine synthase (CrSTR) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0808

Plasmid

#177082

Purpose

MoClo Level 1, position 4, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

tryptophan decarboxylase (CrTDC) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0863

Plasmid

#177081

Purpose

MoClo Level 1, position 5, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

secologanin synthase (CrSLS1) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD1CB0798

Plasmid

#177077

Purpose

MoClo Level 1, position 5, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0790

Plasmid

#177075

Purpose

MoClo Level 1, position 1, transcriptional unit for transient expression of iridoid synthase (CrISY) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

iridoid synthase (CrISY) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD1CB0794

Plasmid

#177073

Purpose

MoClo Level 1, position 3, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geraniol 8-oxidase (CrG8H) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0786

Plasmid

#177071

Purpose

MoClo Level 1, position 3, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geranyl pyrophosphate synthase (PaGPPS1) from Picea abies

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0784

Plasmid

#177069

Purpose

MoClo Level 1, position 1, transcriptional unit for transient expression of P19 suppressor of gene silencing from Tomato Bushy Stunt Virus driven by nos promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

P19 suppressor of gene silencing from Tomato Bushy Stunt Virus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD1CB0776

Plasmid

#177066

Purpose

MoClo Level 1, position 2, transcriptional unit for transient expression of strictosidine synthase (CrSTR) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

strictosidine synthase (CrSTR) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD1CB0774

Plasmid

#177064

Purpose

MoClo Level 1, position 1, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

tryptophan decarboxylase (CrTDC) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0772

Plasmid

#177062

Purpose

MoClo Level 1, position 6, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

secologanin synthase (CrSLS1) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD1CB0768

Plasmid

#177058

Purpose

MoClo Level 1, position 4, transcriptional unit for transient expression of 7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD1CB0767

Plasmid

#177057

Purpose

MoClo Level 1, position 4, transcriptional unit for transient expression of 7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD1CB0124

Plasmid

#177056

Purpose

MoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD1CB0123

Plasmid

#177055

Purpose

MoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD1CB0122

Plasmid

#177054

Purpose

MoClo Level 1, position 2, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD1CB0120

Plasmid

#177052

Purpose

MoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein-like (NmMLPL) from Nepeta mussinii driven by 35S promoter; contains a chloroplast transit peptide

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

major latex protein-like (NmMLPL) from Nepeta mussinii

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD1CB0116

Plasmid

#177048

Purpose

MoClo Level 1, position 5, transcriptional unit for transient expression of 8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD1CB0787

Plasmid

#177072

Purpose

MoClo Level 1, position 4, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geraniol synthase (CrGES) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD1CB0871

Plasmid

#177087

Purpose

MoClo Level 1, position 2, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

secologanin synthase (CrSLS1) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD1CB0796

Plasmid

#177074

Purpose

MoClo Level 1, position 4, transcriptional unit for transient expression of 8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD1CB0114

Plasmid

#177046

Purpose

MoClo Level 1, position 4, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geraniol 8-oxidase (CrG8H) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD1CB0104

Plasmid

#177038

Purpose

MoClo Level 1, position 6, transcriptional unit for transient expression of P19 suppressor of gene silencing from Tomato Bushy Stunt Virus driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

P19 suppressor of gene silencing from Tomato Bushy Stunt Virus

Use

Synthetic Biology

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAP12008

Plasmid

#177036

Purpose

Level 0 promoter and 5'UTR part for MoClo assembly, GGAG-CCAT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

Cauliflower Mosaic Virus (CaMV) 35S promoter and Tobacco Mosaic Virus (TMV) omega 5' UTR

Use

Synthetic Biology

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CM0065

Plasmid

#177019

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

1-deoxy-D-xylulose 5-phosphate synthase

Use

Synthetic Biology

Mutation

transit peptide removed, amplified from cDNA, con…

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
E1_pENTR_L1-L4_NI_G1333-DddtoxA-C

Plasmid

#171724

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
E1_pENTR_L1-L4_NN_G1333-DddtoxA-C

Plasmid

#171728

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N-termini of TALEN, half of a cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
E3_pENTR_L3-L2_NG_G1333-DddtoxA-C

Plasmid

#171732

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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