We narrowed to 4,223 results for: 424

1. pLenti/TO/EYFP-P2A-IDH1(HA)

   Plasmid

   #122482

   Purpose

   A lentiviral vector (pLenti6.3/TO/DEST) expresses EYFP, P2A, and IDH1 C-terminally tagged with HA

   Depositor

   Eric Huang

   Article

   Tiburcio et al Acta Neuropathol. 2018 Feb;135(2):285-29

   Insert

   isocitrate dehydrogenase 1 (IDH1 Human)
   

   Use

   Lentiviral

   Tags

   HA and P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pD649-HAsp-DNER-COMP5AP-AviTag-9xHis

   Plasmid

   #157490

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   DNER (DNER Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXS-IRES-BLAST flag-CAD E1368A

   Plasmid

   #188141

   Purpose

   Expresses C-terminal flag-tagged human CAD E1368A in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. ATX3-77Q-R388G

   Plasmid

   #184251

   Purpose

   Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal)

   Depositor

   Sandra Macedo Ribeiro

   Article

   Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

   Insert

   ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track and point mutation R388G (ATXN3 Human, Synthetic)
   

   Use

   Tags

   6x His-Tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   C-terminal codon optimization for protein expres…

   Promoter

   T7 promoter

   Available since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. HisGB1-CITED2(216-269)+CBPTAZ1(340-439)

   Plasmid

   #173761

   Purpose

   Bacterial coexpression of human CITED2 CTAD with CBP TAZ1

   Depositor

   Peter Wright

   Article

   Berlow et al Nature. 2017 Mar 16;543(7645):447-451. d

   Inserts

   CITED2 (216-269) (CITED2 Human)
   CBP TAZ1 (340-439) (Crebbp Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXS-IRES-BLAST flag-CAD S1396A

   Plasmid

   #188126

   Purpose

   Expresses C-terminal flag-tagged human CAD S1396A in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR223_NRAS_WT

   Plasmid

   #82150

   Purpose

   Gateway Donor vector containing NRAS , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   NRAS (NRAS Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. NES-EGFP-PH-BTKx1

   Plasmid

   #183651

   Purpose

   PtdIns(3,4,5)P3 biosensor

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   BTK (BTK Human, Frog)
   

   Use

   Tags

   X. laevis map2k1.L(32-44)-EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 2-170

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTR-341 pUC19-tNGFR-P2A-PDCD1 N

   Plasmid

   #186074

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   PDCD1 (PDCD1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-IDH1-P33S

    Plasmid

    #116409

    Purpose

    Lentiviral expression of IDH1 P33S

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    P33S

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-myc-AP1S3 F4C

    Plasmid

    #58293

    Purpose

    mammalian expression of AP1S3 F4C mutant

    Depositor

    Francesca Capon

    Article

    Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

    Insert

    AP1S3 (AP1S3 Human)
    

    Use

    Tags

    6xHis and myc

    Expression

    Mammalian

    Mutation

    F4C: c.11T>G, p.Phe4Cys

    Promoter

    CMV

    Available since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

12. R713-M52-303: CMV51p> Halotag-NRasHVR

    Plasmid

    #159686

    Purpose

    Mammalian protein expression of NRAS HVR (Hypervariable region)-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-NRasHVR (NRAS Human)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-myc-AP1S3 R33W

    Plasmid

    #58294

    Purpose

    mammalian expression of AP1S3 R33W mutant

    Depositor

    Francesca Capon

    Article

    Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

    Insert

    AP1S3 (AP1S3 Human)
    

    Use

    Tags

    6xHis and myc

    Expression

    Mammalian

    Mutation

    R33W: c.97C>T,p.Arg33Trp

    Promoter

    CMV

    Available since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pLKO.1-TRC.mKO2_shGLS

    Plasmid

    #110319

    Purpose

    shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene and express monomeric Kusabira-Orange2

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    GLS Glutaminase (GLS Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q25

    Plasmid

    #111743

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q25 (polyQ repeat)

    Promoter

    CMV and P10

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. mRFP-FKBP-INPP5E(D556A)

    Plasmid

    #183678

    Purpose

    Recruitable human type IV 5-phosphatase enzyme (catalytically inactive)

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    INPP5E (INPP5E Human)
    

    Use

    Tags

    mRFP-FKBP12(1-108)

    Expression

    Mammalian

    Mutation

    C641A, D556A

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223_FAIM_WT_V5

    Plasmid

    #82930

    Purpose

    Gateway Donor vector containing FAIM, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, Matthew Meyerson

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    FAIM (FAIM Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Dmap1(SDM)

    Plasmid

    #117139

    Purpose

    Expression vector encoding BFP-BSD-HA-Dmap1 resistant against gRNA encoded by pKLV2-U6(gDmap1 v4)--PGK-Puro-BFP

    Depositor

    Kosuke Yusa

    Article

    Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

    Insert

    HA-Dmap1 (mutagenised) (Dmap1 Synthetic)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    modified BFP, modified Dmap1 CDS

    Promoter

    human U6 promoter, human EF1a promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q85

    Plasmid

    #111736

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q85 (polyQ repeat)

    Promoter

    CMV and P10

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA4/TO/Strep-HA-ZAKalpha_deltaS

    Plasmid

    #141197

    Purpose

    Expresses Strep-HA-ZAKalpha_deltaS in mammalian cells, can be used to make inducible cell lines

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Use

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    deletion of amino acids 670-712

    Promoter

    CMV

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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