We narrowed to 43,874 results for: Ina;

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pET3a-FruK

Plasmid

#186256

Purpose

Overexpression of FruK for purification

Depositor

Liskin Swint-Kruse, Emile Van Schaftingen

Article

Veiga-da-Cunha et al Protein Expr Purif. 2000 Jun;19(1):48-52

Insert

1-phosphofructokinase (fruK E. coli)

Expression

Bacterial

Promoter

T7

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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C453-E18: attB4-polyhedrinp>-attB5

Plasmid

#162977

Purpose

Gateway attB4/attB5 entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E21: attB4-FerHp>-attB5

Plasmid

#162978

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Ferritin heavy chain promoter

Use

Synthetic Biology

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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C417-E10: attB4-polyhedrinp>3xFLAG-nostop-attB1r

Plasmid

#162936

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of 3xFLAG tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Triple FLAG epitope tag fusion, AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r

Plasmid

#162937

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6 fusion, AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3_dPKA-CAT1N17-mVenus

Plasmid

#168497

Purpose

Mammalian expression of the N-terminal 17 residues of Drosophila PKA catalytic subunit 1 C-terminally tagged by monomeric Venus.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

N-terminal 17 residues of Drosophila PKA catalytic subunit 1 C-terminally tagged by monomeric Venus

Expression

Mammalian

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-CβN15-mVenus

Plasmid

#168496

Purpose

Mammalian expression of the N-terminal 15 residues of mouse PKA catalytic subunit β C-terminally tagged by monomeric Venus.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

N-terminal 15 residues of mouse PKA catalytic subunit β C-terminally tagged by monomeric Venus

Expression

Mammalian

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E25: attB4-mPol2p>-attB5

Plasmid

#162981

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the murine RNA polymerase 2 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

murine RNA polymerase 2 promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C417-E06: attB4-polyhedrinp>MBP-nostop-attB1r

Plasmid

#162935

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of MBP tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Maltose-binding protein fusion, AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E09: attB4-Grp78p>-attB5

Plasmid

#162974

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human Grp78 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Glucose response protein 78 promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E11: attB4-Grp78p>-attB1r

Plasmid

#162970

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Glucose response protein 78 promoter

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E10: attB4-FerHp>-attB1r

Plasmid

#162969

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Ferritin heavy chain promoter

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C417-E01: attB4-polyhedrinp>His6-nostop-attB1r

Plasmid

#162932

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6 fusion with AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C415-E01: attB4-stuffer-attB1r

Plasmid

#162931

Purpose

Gateway attB4-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-rsAKARev(T/A)

Plasmid

#166232

Purpose

Expresses reversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant (rsAKARev(T/A)) in mammalian cells

Depositor

Peter Dedecker

Article

Roebroek et al Nat Commun. 2021 Mar 31;12(1):2005. doi:

Insert

Reversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant

Expression

Mammalian

Mutation

changed Threonine 506 in rsAKARev to Alanine

Promoter

CMV

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD

Plasmid

#162811

Purpose

Control STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning.

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

BsmBI_(C1A)-Intein_DD

Use

Piggybac

Expression

Mammalian

Mutation

Intein(Cys1,Ala)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

Plasmid

#162805

Purpose

STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI-HA_IRES_Blasticidin

Use

Piggybac

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pME-QUASM1:GFPNLS-SV40pA

Plasmid

#155116

Purpose

Middle entry vector containing QUASM1 element upstream of GFP-NLS.

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:GFPNLS

Use

Gateway middle entry vector

Promoter

QUASM1-E1b

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pMP89b CvTA V124N

Plasmid

#141207

Purpose

Chromobacterium violaceum transaminase with enhances affinity for PLP. TEV cleaving site after the N-terminal Histag.

Depositor

Francesca Paradisi

Article

Roura Padrosa et al Front Bioeng Biotechnol. 2019 Oct 18;7:2

Insert

CvTA

Expression

Bacterial

Mutation

V124N

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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pEM03

Plasmid

#135417

Purpose

Expression of Hygromycin resistance from Spizellomyces Hsp70 promoter

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunHsp70pr

Expression

Bacterial and Yeast

Promoter

Hsp70pr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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