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pMK1224

Plasmid

#84221

Purpose

Lentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, BFP

Depositor

Martin Kampmann, Jonathan Weissman

Publication

Kampmann et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pD2eGFP-5xUTR

Plasmid

#31121

Depositor

Christopher Sullivan

Publication

Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

Insert

5xoligonucleotides eGFPutr

Expression

Mammalian

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
P2*-IRES-tauLacZ LTNL TV

Plasmid

#15645

Depositor

Peter Mombaerts

Publication

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

P2*-IRES-tauLacZ LTNL B6 TV

Use

High copy number

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
M50-IRES-GFP-IRES-tauLacZ LNL TV

Plasmid

#15646

Depositor

Peter Mombaerts

Publication

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

M50-IRES-GFP-IRES-tauLacZ LNL TV

Use

High copy number

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
P3->P2-IRES-tauGFP LNL TV

Plasmid

#15650

Depositor

Peter Mombaerts

Publication

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

P3->P2-IRES-tauGFP LNL 129 TV

Use

High copy number

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
HybridH-IRES-tauGFP LNL TV

Plasmid

#15656

Depositor

Peter Mombaerts

Publication

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

HybridH-IRES-tauGFP LNL TV

Use

High copy number

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
HybridJ-IRES-tauGFP LNL TV

Plasmid

#15658

Depositor

Peter Mombaerts

Publication

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

HybridJ-IRES-tauGFP LNL TV

Use

High copy number

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
R619-M63-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2

Plasmid

#166010

Purpose

mammalian expression of SARS-CoV-2 soluble spike trimer protein

Depositor

Dominic Esposito

Publication

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) (S )

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pACT1:Cas9-GFP, U6:sgTK

Plasmid

#122852

Purpose

Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene

Depositor

David Sibley

Publication

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Inserts

Cas9-GFP
U6-sgTK

Use

CRISPR; Cas9-gfp plasmid for genome editing of cr…

Tags

eGFP

Promoter

Cryptosporidium parvum U6 and Cryptosporidium par…

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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LLP453 pEf1a-NLS-aGCN4-mCherry

Plasmid

#100945

Purpose

mCherry fusion to single change variable fragment antibody GCN4 for use in SunTag system , with 3xTy1 tag

Depositor

Ryan Lister

Publication

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

scFvGCN4, sfGFP, GB1, mCherry

Tags

3xTy1

Expression

Mammalian

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pDest27-mBIN1+13+17

Plasmid

#61097

Purpose

Express N-terminal GST tagged-mouse BIN1+13+17 in mammalian cells

Depositor

Robin Shaw

Publication

Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

Insert

mouse BIN1 with exon 13 and 17, excluding exon 7, 11, 14-16

Tags

GST

Expression

Mammalian

Promoter

CMV

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
EF1a-L274GMmMetRS-T2A-mCherry

Plasmid

#220800

Purpose

Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance

Depositor

Elizabeth Kirby

Publication

Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18

Insert

Mars1 (Mars1 Mouse)

Use

Lentiviral

Tags

2xFLAG and T2A-mCherry

Mutation

mutation in MetRS to change aa 274 from L to G

Promoter

EF1a

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PIK4CB

Plasmid

#20578

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PIK4CB (PI4KB Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag AAK1

Plasmid

#20133

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AAK1 (AAK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pPB_TRE3G_scFV-sfGFP-Dnmt3a3lCD

Plasmid

#235576

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dnmt3a3L fused with scFV to programme DNA methylation

Depositor

Jamie Hackett

Publication

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Dnmt3a-Dnmt3L (Dnmt3a, Dnmt3l Mouse)

Use

CRISPR

Available since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pSF11-wElectra2

Plasmid

#184933

Purpose

Codon optimized blue fluorescent protein Electra2 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Publication

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra2

Expression

Worm

Promoter

tag-168

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-dfROX

Plasmid

#137170

Purpose

Lentiviral transfer vector for expression of roGFP2 and HyPer Red

Depositor

Shaun McCullough

Publication

Faber et al Toxicol Sci. 2020 Jun 11. pii: 5856111.

Insert

roGFP2-2A-HyPer Red

Use

Lentiviral

Promoter

EF1a

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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SPDK3889(TRV-RNA2-pPEBV-MCS-AtMet-tRNA)

Plasmid

#149277

Purpose

Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

Depositor

Savithramma Dinesh-Kumar

Publication

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter and AtMet-tRNA mobile RNA sequence

Use

T-dna vector

Mutation

T2318C (DNA) in PEBV coat protein sequence

Available since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6017nes

Plasmid

#108655

Purpose

A PKA biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Publication

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-PKA-EV

Tags

XFP

Expression

Mammalian

Promoter

pCAGGS

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C

Plasmid

#90014

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6C

Depositor

Julien Béthune

Publication

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Ago2
NBirA*-TNRC6C

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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