We narrowed to 1,621 results for: nif

1. Flag-FOXE1

   Plasmid

   #153120

   Purpose

   Expression of Flag tagged human FOXE1 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXE1 (FOXE1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMA_Int

   Plasmid

   #110096

   Purpose

   E. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Insert

   mycophage integrase
   

   Use

   Required for transformation of attb integrating v…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Flag-FOXD3

   Plasmid

   #153118

   Purpose

   Expression of Flag tagged human FOXD3 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXD3 (FOXD3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pACEC3GH (Apr)

   Plasmid

   #110085

   Purpose

   Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP-C-SLC35B2

   Plasmid

   #155037

   Purpose

   Transient expression of EGFP-SLC35B2

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   SLC35B2 (SLC35B2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ

   Plasmid

   #192380

   Purpose

   To test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Flag-FOXN3

   Plasmid

   #153139

   Purpose

   Expression of Flag tagged human FOXN3 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXN3 (FOXN3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET22b-ecDHFR-G51PEKN

   Plasmid

   #118582

   Purpose

   Bacterial expression of E.coli DHFR G51PEKN mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

   Insert

   DHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G51 mutated to P51; Glu-Lys-Asn residues inserted…

   Promoter

   T7

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pST39-His/Vps27(345-440) - Hse(275-375)

   Plasmid

   #17638

   Depositor

   James Hurley

   Article

   Prag et al Dev Cell. 2007 Jun . 12(6):973-86.

   Insert

   His/Vps27(345-440) – Hse1(275-375) (VPS27, HSE1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

10. NFAST(low)-Halo-ER

    Plasmid

    #214419

    Purpose

    ER localization of low-affinity NFAST fused to Halo-ER

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspA-N
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUC57-min5UTR-hRluc-A60

    Plasmid

    #182638

    Purpose

    Transcription template plasmid for a minimal 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

    Depositor

    Stephen Floor

    Article

    Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

    Insert

    Renilla luciferase,
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FUiLMO2W

    Plasmid

    #129480

    Purpose

    3rd-generation lentiviral vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical

    Depositor

    Robert Gross

    Article

    Tung et al Sci Rep. 2015 Sep 24;5:14366. doi: 10.10

    Insert

    iLMO2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-iLMO2

    Plasmid

    #129474

    Purpose

    Mammalian expression vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical

    Depositor

    Robert Gross

    Article

    Tung et al Sci Rep. 2015 Sep 24;5:14366. doi: 10.10

    Insert

    iLMO2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LLP754_L2pV1_NOT_FRT_Rluc_1IF_lacZ

    Plasmid

    #192399

    Purpose

    Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-Rluc-FRT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LLP753_L2pV1_NOT_FRT_Rluc_0I_lacZ

    Plasmid

    #192398

    Purpose

    On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-Rluc-FRT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LLP730_L2_pV01_0I_FRT-OCS-TB-rLUC_TCTP-fLUC

    Plasmid

    #192373

    Purpose

    To test for the repressive potential of the OCS and Transcription Block (TB) fusion with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-TB-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LLP733_L2_pV01_0I_FRT-uORF-rLUC_TCTP-fLUC

    Plasmid

    #192376

    Purpose

    To test for the repressive potential of the conserved peptide uORF from AT1G36730 with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-uORF-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LLP727_L2_pV01_0I_TMV-rLUC_TCTP-fLUC

    Plasmid

    #192370

    Purpose

    To control for variation introduced to the gene circuits, Rluc expression not affected by the introduction of terminators or recombination target sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ

    Plasmid

    #192375

    Purpose

    To test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-35Ster-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

    Plasmid

    #192374

    Purpose

    To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCStrunc-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZ

    Plasmid

    #192385

    Purpose

    To test for if Flp is able to de-repress a circuit with B3RT, to rule out cross-reactivity between these recombinases.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::B3RT-OCS-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ

    Plasmid

    #192386

    Purpose

    To test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ

    Plasmid

    #192404

    Purpose

    On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    B3RT-Act2-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ

    Plasmid

    #192401

    Purpose

    Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::B3RT-Rluc-B3RT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LLP755_L2pV1_NOT_B3RT_Rluc_0I_lacZ

    Plasmid

    #192400

    Purpose

    On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::B3RT-Rluc-B3RT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZ

    Plasmid

    #192402

    Purpose

    On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    FRT-Act2-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ

    Plasmid

    #192405

    Purpose

    Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    B3RT-Act2-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LLP713_L2pV3_NOT_FRT_Rluc_1IF_lacZ

    Plasmid

    #192403

    Purpose

    Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    FRT-Act2-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ

    Plasmid

    #192381

    Purpose

    To test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ

    Plasmid

    #192379

    Purpose

    To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge