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pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]

Plasmid

#168173

Purpose

Expression of BeCyclOp in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Gao et al Nat Commun. 2015 Sep 8;6:8046. doi: 10.1

Insert

BeCyclOp (codon optimized for C. elegans)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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Flag TSC2 2A(S939A + S981A)

Plasmid

#12131

Depositor

Cheryl Walker

Article

Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

Insert

TSC2 S939A S981A (TSC2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S939A. S981A.

Promoter

Available since

July 6, 2006

Availability

Academic Institutions and Nonprofits only
pBSc + MShab

Plasmid

#16146

Depositor

Larry Salkoff

Article

Pak et al J Neurosci. 1991 Mar . 11(3):869-80.

Insert

Mus musculus potassium voltage gated channel (Kcnb1 Fly, Mouse)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pBSc polyA + Shal1

Plasmid

#16196

Depositor

Larry Salkoff

Article

Wei et al Science. 1990 May 4. 248(4955):599-603.

Insert

Drosophila melanogaster Shaker cognate l (Shal Fly)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pASH3 [pmyo-3::BeCNG1-YFP]

Plasmid

#168167

Purpose

Expression of BeCNG1-YFP in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

BeCNG1-YFP

Use

Tags

YFP

Expression

Worm

Mutation

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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DAP (DOL-AP) (CC#44)

Plasmid

#15164

Depositor

Connie Cepko

Article

Fields-Berry et al Proc Natl Acad Sci U S A. 1992 Jan 15. 8

Insert

PLAP (ALPP Human)

Use

Retroviral; Avian expression

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
Flag-M4-AR-K313A-K318A

Plasmid

#171260

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor Lysine mutant: AR-Lys313Ala-Lys318Ala (AR-K313A-K318A)

Depositor

Steven Balk

Insert

3xFlag-tagged human androgen receptor Lys313Ala-Lys318Ala mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Lys313Ala-Lys318Ala (AR-K313A-K318A)

Promoter

CMV

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-SpDcp2opt_1-242-E147Q_AD

Plasmid

#148546

Purpose

Bacterial Expression of SpDcp2opt_1-242-E147Q

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp2opt_1-242-E147Q (dcp2 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-K630A-K632A

Plasmid

#171263

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor Lysine mutant: AR-Lys630Ala-Lys632Ala (AR-K630A-K632A)

Depositor

Steven Balk

Insert

3xFlag-tagged human androgen receptor Lys630Ala-Lys632Ala mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Lys630Ala-Lys632Ala (AR-K630A-K632A)

Promoter

CMV

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBSc + MShal

Plasmid

#16166

Depositor

Larry Salkoff

Article

Pak et al Proc Natl Acad Sci U S A. 1991 May 15. 8

Insert

Mus musculus potassium voltage-gated channel (Kcnd1 Mouse)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pSW149

Plasmid

#122248

Purpose

pTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.

Depositor

Sarah Walker

Article

Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18

Insert

TIF4631 (TIF4631 Budding Yeast)

Use

Tags

Intein-Chitin-binding domain

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAB16 (pmyo-3::QuasAr)

Plasmid

#130272

Purpose

Expresses the voltage sensor QuasAr in BWMs of C. elegans.

Depositor

Alexander Gottschalk

Article

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

QuasAr

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR1A hu_ncOGT WT

Plasmid

#154281

Purpose

Entry vector encoding non-tagged O-GlcNAc Transferase

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x FLAG and mKate2

Expression

Mutation

WT OGT

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA flag-Pcbp2

Plasmid

#184028

Purpose

Codon optimized Pcbp2 clone with N-terminal flag tag

Depositor

Peter Stoilov

Article

Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

Insert

Pcbp2 (Pcbp2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Codon optimized

Promoter

CMV

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 SV8

Plasmid

#184011

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286).

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Wild type cDNA

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTH33 [pmyo-3::YFP-BeCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168169

Purpose

Expression of YFP-BeCyclOp(A-2x) in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)

Use

Tags

YFP

Expression

Worm

Mutation

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRA-33-MC_hammer

Plasmid

#138989

Purpose

IVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGG-PIP04

Plasmid

#196742

Purpose

Contains RALF23 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

ARABIDOPSIS RAPID ALKALINIZATION FACTOR 23 (RALF23 Mustard Weed)

Use

Greengate (gg) entry vector

Tags

Expression

Mutation

615 C->G

Promoter

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-W741C-S81A-P82A

Plasmid

#171250

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor Serine-Proline mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala-Pro82Ala (AR-W741C-S81A-P82A)

Depositor

Steven Balk

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala-Pro82Ala mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser81Ala-Pro82Ala (AR-W741C-S81A-P82…

Promoter

CMV

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-W741C-S81A-P82G

Plasmid

#171251

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor Serine-Proline mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala-Pro82Gly (AR-W741C-S81A-P82G)

Depositor

Steven Balk

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala-Pro82Gly mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser81Ala-Pro82Gly (AR-W741C-S81A-P82…

Promoter

CMV

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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