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We narrowed to 4,919 results for: codon optimized

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Showing: 341 - 360 of 4919 results

  1. MSP1673

    Plasmid
    #65769
    Purpose
    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    Use
    CRISPR
    Tags
    NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    65769-plasmid-map-sequence-id-112659

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  4. pminiTol2-5xUAS-hsp-sfGFP-zP2A-tetoff-zN2cG-zP2A-zTVA (UGNT-A)

    Plasmid
    #240272
    Purpose
    Helper plasmid for zebrafish rabies virus based-circuit tracing
    Depositor
    Xufei Du
    Publication
    Chen et al eLife 2024 [Reviewed Preprint] 13:RP1008
    Inserts
    N2cG (codon-optimized for Danio rerio)
    sfGFP (codon-optimized for Danio rerio)
    TVA (codon-optimized for Danio rerio)
    tetoff (codon-optimized for Danio rerio)
    Use
    Zebrafish expression
    Available since
    April 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    240272-plasmid-map-sequence-id-485525

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  5. MSP977

    Plasmid
    #65774
    Purpose
    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E mutation in Cas9
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65774-plasmid-map-sequence-id-112656

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  6. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid
    #174138
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174138-plasmid-map-sequence-id-341915

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  7. pCAG-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1081)

    Plasmid
    #114094
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RR (S542R/K607R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    S542R and K607R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114094-plasmid-map-sequence-id-220561

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  8. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

    Plasmid
    #185916
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8.20m-nSpG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185916-plasmid-map-sequence-id-367354

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  10. pN7HELIX

    Plasmid
    #190662
    Purpose
    Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
    Expression
    Mammalian
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    CMV
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    190662-plasmid-map-sequence-id-385664

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  12. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Kiran Musunuru
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    64709-plasmid-map-sequence-id-322473

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  13. pCAG-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1065)

    Plasmid
    #114092
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a (S542R/K548V/N552R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    S542R, K548V and N552R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114092-plasmid-map-sequence-id-230539

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  16. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

    Plasmid
    #174091
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Promoter
    pCMV and T7
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174091-plasmid-map-sequence-id-341917

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  17. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid
    #174139
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174139-plasmid-map-sequence-id-341913

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  18. MIP 247 CoMiP 4in1 with shRNA p53

    Plasmid
    #63726
    Purpose
    To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).
    Depositor
    Mark Kay, Joseph Wu
    Publication
    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103
    Insert
    Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC, TP53 Human)
    Tags
    IRES-dtomato
    Expression
    Mammalian
    Mutation
    codon-optimised
    Promoter
    SFFV for Pct4, Klf4, Sox2 and c-myc; U6 for p53
    Available since
    Aug. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    63726-plasmid-map-sequence-id-438433

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  19. pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)

    Plasmid
    #185915
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8.20m-nSpCas9-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185915-plasmid-map-sequence-id-367353

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  20. pTEF:ATP

    Plasmid
    #92179
    Purpose
    HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.
    Depositor
    Matthias Heinemann
    Publication
    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi
    Insert
    ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
    Use
    Integration and expression into yeast.
    Tags
    Yeast codon optimized mseCFP (donor) and cp173-mV…
    Expression
    Yeast
    Promoter
    TEF1p
    Available since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    92179-plasmid-map-sequence-id-181610

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Showing: 341 - 360 of 4919 results