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We narrowed to 5,049 results for: golden gate

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Showing: 341 - 360 of 5049 results

  1. pOPS0750

    Plasmid
    #115511
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Constitutive promoter pNeo pOGG001
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    115511-plasmid-map-sequence-id-223075

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  2. pOPS0314

    Plasmid
    #115507
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celB
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Constitutive promoter pNeo pOGG001
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115507-plasmid-map-sequence-id-224284

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  3. pOPS0253

    Plasmid
    #115505
    Purpose
    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Rlv3841pNifH pOGG082, gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115505-plasmid-map-sequence-id-224288

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  5. L0I80_Bxb1mut(noBsaI)_GG1-GG4

    Plasmid
    #219715
    Purpose
    Level 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG1-Bxb1(noBsaI0)-GG4
    Use
    Synthetic Biology
    Available Since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219715-plasmid-map-sequence-id-435740

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  8. pGGG-TaBSS1-GUS(int)

    Plasmid
    #242013
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of transgenic GUS (with introns) driven by the Basal Spikelet Specific 1 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    GUS_with_introns
    Expression
    Plant
    Promoter
    BSS1
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242013-plasmid-map-sequence-id-504513

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  9. pGGG-TaBSS2-tdTom

    Plasmid
    #242018
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of transgenic tdTomato driven by the Basal Spikelet Specific 2 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    tdTomato
    Tags
    NLS
    Expression
    Plant
    Promoter
    BSS2
    Available Since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    242018-plasmid-map-sequence-id-492286

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  10. pGGG-TaBSS1-tdTom

    Plasmid
    #242014
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of transgenic tdTomato driven by the Basal Spikelet Specific 1 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    tdTomato
    Tags
    NLS
    Expression
    Plant
    Promoter
    BSS1
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242014-plasmid-map-sequence-id-487879

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  11. pXZ13lacZalphaKanR

    Plasmid
    #160230
    Purpose
    Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.
    Depositor
    Ian Duncan
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    160230-plasmid-map-sequence-id-314765

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  12. pDM026

    Plasmid
    #216808
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216808-plasmid-map-sequence-id-429385

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  13. pDM028

    Plasmid
    #216809
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and hph selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216809-plasmid-map-sequence-id-430135

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  14. pDM068

    Plasmid
    #216811
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216811-plasmid-map-sequence-id-430142

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  15. pDM030

    Plasmid
    #216810
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216810-plasmid-map-sequence-id-430137

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  18. p028g34-3Cs

    Plasmid
    #249636
    Purpose
    Clone the cleavage site for 3C protease between the N-terminal tag and the CDS of the protein to be expressed using pET28g backbone, using fusion sites 3 and 4
    Depositor
    Isabel Abreu
    Article
    Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi
    Insert
    Recognition site for 3C protease
    Expression
    Bacterial
    Available Since
    April 10, 2026
    Availability
    Academic Institutions and Nonprofits only
    249636-plasmid-map-sequence-id-506116

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  19. p028g34-TEVs

    Plasmid
    #249633
    Purpose
    Clone the cleavage site for TEV protease between the N-terminal tag and the CDS of the protein to be expressed using pET28g backbone, using fusion sites 3 and 4
    Depositor
    Isabel Abreu
    Article
    Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi
    Insert
    Recognition site for TEV protease
    Expression
    Bacterial
    Available Since
    April 10, 2026
    Availability
    Academic Institutions and Nonprofits only
    249633-plasmid-map-sequence-id-506112

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Showing: 341 - 360 of 5049 results