We narrowed to 3,767 results for: golden gate

1. GGE005

   Plasmid

   #120748

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_GAPdh)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   P2_GAPdh
   

   Use

   Cloning/assembly plasmid

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. GGE004

   Plasmid

   #120749

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_PGM)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   P2_PGM
   

   Use

   Cloning/assembly plasmid

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMA_Auxiliary 4B

   Plasmid

   #102713

   Purpose

   Provides the End-to-End linker 4B which assists Level 2 cloning in Mobius Assembly

   Depositor

   Naomi Nakayama

   Article

   Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

   Insert

   Linker sequence
   

   Use

   Synthetic Biology

   Available Since

   March 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGGEV_4'_+nls-mCherry-Flag-_+1_BK+

   Plasmid

   #49313

   Purpose

   Golden Gate entry vector: Position 4', Flag tagged nls-mCherry with start and stop codon

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Insert

   nls-mCherry-Flag
   

   Use

   Cloning vector

   Available Since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGGEV_2_+nls-eGFP-HA-_+1_BK+

   Plasmid

   #49312

   Purpose

   Golden Gate entry vector: Position 2, HA tagged nls-EGFP with start and stop codon

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Insert

   nls-EGFP-HA
   

   Use

   Cloning vector

   Available Since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-GG-gRNA

   Plasmid

   #226860

   Purpose

   Human gRNA expression vector backbone with BsmBI Golden Gate site

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   Sp-gRNA
   

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICSL50009

   Plasmid

   #50309

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   C terminal HA tag (6x Human influenza hemagglutinin)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICSL30008

   Plasmid

   #50300

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   N terminal HA tag (6x Human influenza hemagglutinin)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICSL50004

   Plasmid

   #50316

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS, mCherry variant of RFP (Discosoma sp.)
   

   Expression

   Plant

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICSL30003

    Plasmid

    #50304

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, no stop codon, mCherry variant of RFP (Discosoma sp.)
    

    Expression

    Plant

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. mTB3-BC2 nanobody

    Plasmid

    #210208

    Purpose

    Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

    Depositor

    Brian Kay

    Article

    Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

    Insert

    BC2 Nanobody
    

    Expression

    Bacterial

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICSL30009

    Plasmid

    #50301

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    N terminal Myc tag (4x Myc)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICSL50010

    Plasmid

    #50310

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    C terminal Myc tag (4x Myc)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICH41421

    Plasmid

    #50339

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICH47742:FCP:Cas9YFP

    Plasmid

    #85986

    Purpose

    Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR; Golden gate assembly

    Tags

    SV40 NLS, YFP

    Promoter

    FCP

    Available Since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICH47732:FCP:NAT

    Plasmid

    #85984

    Purpose

    Golden Gate Level 1 cassette encoding a Nat selectable marker under the FCP promoter

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Insert

    NAT
    

    Use

    Golden gate assembly

    Promoter

    FCP

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. GGE114

    Plasmid

    #120731

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Destination vector for the insertion of the assembly. It already has some of the parts needed for transformation in Y. lipolytica

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Type

    Empty backbone

    Use

    Cloning/assembly plasmid

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNLS-LEXY (pDN138)

    Plasmid

    #72657

    Purpose

    golden gate entry vector for mCherry-LEXY tagging of proteins (N-terminal cMyc(P1A) NLS)

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

    Type

    Empty backbone

    Use

    Golden gate entry vector

    Tags

    NLS and mCherry-LEXY

    Expression

    Mammalian

    Promoter

    CMV-F

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICH41414

    Plasmid

    #50337

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    3'UTR, polyadenylation signal/terminator, 35s (Cauliflower Mosaic Virus)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICSL80005

    Plasmid

    #50322

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, variant of turboGFP codon-optimised for plants (Pontellina plumata)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge