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Plasmid#61489Purposeluciferase reporter containing RAB14 3' UTRDepositorInsertRAB14 3' UTR (RAB14 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationcontains position 837-1637 (numbering relative to…PromoterPGKAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTG484E-myc
Plasmid#107471PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G484E decreased transport and surface expression relative to wildtypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationG484EPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTS477F-myc
Plasmid#107470PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S477F loss of transport relative to wildtype, decreased surface expressionDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationS477FPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHL 1405
Plasmid#53010PurposepLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
DsrA sRNA
hfq
sodB
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains -56 to +141 relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-4_W267N
Plasmid#214314PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (CASP4 Human)
UseTagsHis-TEVExpressionBacterialMutationW267NPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-4_R269D
Plasmid#214313PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (CASP4 Human)
UseTagsHis-TEVExpressionBacterialMutationR269DPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP722_pSP15
Plasmid#192435PurposeTo test the relative expression strength of the TCTP1 promoterDepositorInsertTCTP1::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP720_pSP13
Plasmid#192433PurposeTo test the relative expression strength of the 35S promoterDepositorInsert35S::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP721_pSP14
Plasmid#192434PurposeTo test the relative expression strength of the Act2 promoterDepositorInsertAct2::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-GFPrev
Plasmid#158060PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteracpPAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTT439M-myc
Plasmid#107466PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation T439M, decreased surface expression and transport relative to wildtypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationT439MPromoterCMVAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTG384S-myc
Plasmid#107463PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G384S with loss of surface expression relative to wildtypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationG384SPromoterCMVAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTS375L-myc
Plasmid#107462PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S375L with lower surface expression relative to wildtypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationS375LPromoterCMVAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHL 100
Plasmid#52989PurposepLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfpDepositorInsertsOxyS sRNA
fhlA
UseSynthetic BiologyTagsGFP and RBS-mCherryExpressionBacterialMutation−309 to +96 bp relative to start codonPromoterpLlac0-1 and pLtetO-1Available sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 990
Plasmid#52992PurposepLtetO-1:DsrA, pCon:RhyB, pLlacO-1:rpoS::gfpDepositorInsertsDsrA sRNA
RhyB sRNA (competitor)
rpoS
UseSynthetic BiologyTagsgfpExpressionBacterialMutationincludes −150 to +30 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1022
Plasmid#52996PurposepLtetO-1:RhyB, pCon:OxyS, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
OxyS sRNA
sodB
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 802
Plasmid#52991PurposepLtetO-1:OxyS-mCherry, pLlacO-1:ompC::gfpDepositorInsertsOxyS sRNA
ompC
UseSynthetic BiologyTagsGFP and RBS-mCherryExpressionBacterialMutationcontains −81 to +36 bp relative to start codonPromoterpLlacO-1 and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1020
Plasmid#52995PurposepLtetO-1:RhyB, pCon:DsrA, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
DsrA sRNA (competitor)
sodB
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1019
Plasmid#52994PurposepLtetO-1:RhyB, pCon:MicC, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
MicC sRNA (Competitor)
sodB
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1039
Plasmid#53000PurposepLtetO-1:DsrA, pCon:OxyS, pLlacO-1:rpoS::gfpDepositorInsertsDsrA sRNA
OxyS sRNA (competitor)
rpoS
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains −150 to +30 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only