We narrowed to 159,258 results for: addgene

1. lentiMutB3GNT5-blast

   Plasmid

   #208380

   Purpose

   lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   nt 922 c->g (PAM site inactivation)

   Promoter

   EF-1 alpha core promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVPRT-tTR-KRAB

   Plasmid

   #11648

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hPrion, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pL1P5 ZmUbiP:GRF-GIF:NosT

   Plasmid

   #198046

   Purpose

   MoClo Golden Gate Level 1 Position 5. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

   Depositor

   John Innes Centre - Wheat Transformation and Genome Editing, Cristobal Uauy

   Insert

   ZmUbiP::TaGRF4-GIF1::NosT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Zea mays (maize) ubiquitin promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p8389 LentiCRISPRv2 Neo sgNT-1

   Plasmid

   #221649

   Purpose

   Expression of non-targeting control sgRNA

   Depositor

   Elizabeth White

   Article

   Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

   Insert

   non-targeting sgNT-1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6BHD (SETDB1)

   Plasmid

   #119955

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   SETDB1 (SETDB1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 190-410

   Promoter

   T7

   Available since

   Feb. 1, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. tTA_BFP

   Plasmid

   #58854

   Purpose

   Constitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   tTA transcription factor fused to enhanced blue fluorescent protein
   

   Use

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. 53BP1 N-terminal sgRNA

   Plasmid

   #207091

   Purpose

   pX330 based plasmid for expression of Cas9 and the GGGGAGCAGATGGACCCTAC sgRNA to target the 53BP1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   GGGGAGCAGATGGACCCTAC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. iMbody_pSANG10-3F

   Plasmid

   #184496

   Purpose

   Expresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteria

   Depositor

   Marcel Dinger

   Article

   Zeraati et al STAR Protoc. 2023 Nov 21;4(4):102729. do

   Insert

   iMbody
   

   Use

   Tags

   3xFLAG, 6xHis, and pelB leader

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. MXS_CMV::PuroR-bGHpA

   Plasmid

   #62439

   Purpose

   MXS_chaining vector with CMV::PuroR-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   resistance cassette against Puromycin with CMV Promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. p184_LTJ_sgRNACD90.2

    Plasmid

    #82670

    Purpose

    sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting mouse CD90.2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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