We narrowed to 13,333 results for: Mpl;

1. pET-21a(+)-DR RecR(K23A/R27A)

   Plasmid

   #12619

   Depositor

   SeWon Suh

   Article

   Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

   Insert

   Deinococcus radiodurans RecR (recR Deinococcus radiodurans)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Lysine 23 to Alanine, and Arginine 27 to …

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. p218 pCMV-CREM-L

   Plasmid

   #8396

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   CREM-L
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Mutation

   modified Cre, single LOX 511 site

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pLIC.B3.MJ0130m

   Plasmid

   #11528

   Depositor

   Sung-Hou Kim

   Article

   Kim et al Proc Natl Acad Sci U S A. 2005 Mar 1. 10

   Insert

   Type I restriction-modification enzyme (MJ_0130m M. jannaschii)
   

   Tags

   HisTag, TEV cleavable

   Expression

   Bacterial

   Available Since

   April 10, 2006

   Availability

   Academic Institutions and Nonprofits only

4. C077

   Plasmid

   #174346

   Purpose

   Template for making mRNA for overexpressing frog PABPC1 (C-FLAG) in frog oocytes

   Depositor

   David Bartel

   Article

   Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

   Insert

   PABPC1 (pabpc1.S Frog)
   

   Use

   In vitro transcription template

   Tags

   FLAG

   Availability

   Academic Institutions and Nonprofits only

   

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5. C078

   Plasmid

   #174347

   Purpose

   Template for making mRNA for overexpressing frog ePAB (C-FLAG) in frog oocytes

   Depositor

   David Bartel

   Article

   Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

   Insert

   ePAB
   

   Use

   In vitro transcription template

   Tags

   FLAG

   Mutation

   13 silent mutations: G243A, A273G, G339A, C345G, …

   Availability

   Academic Institutions and Nonprofits only

   

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6. C233

   Plasmid

   #174349

   Purpose

   Template for making mRNA for overexpressing frog PABPC1 M161A mutant (C-FLAG) in frog oocytes

   Depositor

   David Bartel

   Article

   Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

   Insert

   PABPC1 (M161A) (pabpc1.S Frog)
   

   Use

   In vitro transcription template

   Tags

   FLAG

   Mutation

   M161A

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_dCas9-KRAB-MeCP2_Blast

   Plasmid

   #221501

   Purpose

   Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   dCas9-KRAB-MeCP2-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRCC-N

   Plasmid

   #81192

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available Since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX_311-KRAB-dCas9

   Plasmid

   #96918

   Purpose

   for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

   Depositor

   David Root, William Hahn, John Doench

   Article

   Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   KRAB

   Expression

   Mammalian

   Mutation

   dCas9

   Promoter

   EF1a

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-MS2-HA-HsSmg5_T

    Plasmid

    #147552

    Purpose

    Mammalian Expression of HsSmg5

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsSmg5 (SMG5 Human)
    

    Expression

    Mammalian

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-FLAG-CaMKIIA

    Plasmid

    #186825

    Purpose

    Expresses FLAG-CaMKIIA in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    Camk2a (Camk2a Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya

    Plasmid

    #186980

    Purpose

    mammalian expression of Cluc-Gluc reporter

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    Cluc, Gluc
    

    Use

    Luciferase

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. YSBE 516 : pNEO_4xFLAG

    Plasmid

    #92082

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, Neo selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    4xFlag
    

    Use

    tagging proteins in cryptococcus neoforman

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

    Plasmid

    #219537

    Purpose

    a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    Cre recombinase (cre )
    NLS-tdMCP-GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

    Plasmid

    #60903

    Purpose

    Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    Cas9 dead
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

    Plasmid

    #60904

    Purpose

    The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJAF205

    Plasmid

    #60407

    Purpose

    Flag-tagged full length GMAP210/Trip11

    Depositor

    Gregory Pazour

    Article

    Follit et al PLoS Genet. 2008 Dec;4(12):e1000315. doi

    Insert

    GMAP210 (Trip11 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

18. His6_MBP_FMRP_P451S

    Plasmid

    #198693

    Purpose

    Expression of human FMRP protein in E. coli

    Depositor

    Simpson Joseph

    Article

    Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

    Insert

    FMR1 (FMR1 Human)
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed proline 451 to serine

    Promoter

    T7

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX459-sgAAVS1

    Plasmid

    #184403

    Purpose

    pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    AAVS1-specific sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-MS2-HA-HsRoquin1_AE

    Plasmid

    #148611

    Purpose

    Mammalian Expression of HsRoquin1

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin1 (RC3H1 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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