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pEN_TT Linker

Plasmid

#192307

Purpose

Gateway entry vector for cloning

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

Linker

Use

Gateway Cloning

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_485-938_H

Plasmid

#146457

Purpose

Bacterial Expression of NcQDE2_485-938

Depositor

Elisa Izaurralde

Article

Boland et al EMBO Rep. 2010 Jul;11(7):522-7. doi: 10.

Insert

NcQDE2_485-938

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_506-938_H

Plasmid

#146458

Purpose

Bacterial Expression of NcQDE2_506-938

Depositor

Elisa Izaurralde

Article

Boland et al EMBO Rep. 2010 Jul;11(7):522-7. doi: 10.

Insert

NcQDE2_506-938

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_517-938_H

Plasmid

#146459

Purpose

Bacterial Expression of NcQDE2_517-938

Depositor

Elisa Izaurralde

Article

Boland et al EMBO Rep. 2010 Jul;11(7):522-7. doi: 10.

Insert

NcQDE2_517-938

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-myc-OGA(GS-544)C181_NES

Plasmid

#193998

Purpose

plasmid encoding myc-tagged OGA(GS-544)C181_NES

Depositor

Christina Woo

Article

Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

Insert

myc-OGA(GS-544)C181_NES

Tags

myc

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2Ab

Plasmid

#183084

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAB174

Plasmid

#101673

Purpose

Opto-T7RNAP*(179)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(179)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB144

Plasmid

#101665

Purpose

Opto-T7RNAP*(69)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(69)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB153

Plasmid

#101664

Purpose

Opto-T7RNAP*(600)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(600)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSKA322

Plasmid

#101676

Purpose

empty backbone with chloramphenicol resistance

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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SZ1-GST-mCherry-RGG

Plasmid

#176646

Purpose

SynZip1 linked surfactant protein that localizes to RGG condensate interface

Depositor

Daniel Hammer

Article

Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

Insert

SZ1-GST-mCherry-RGG

Expression

Bacterial

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEMF034

Plasmid

#157631

Purpose

sMHT-113

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-SYNZIP17
SYNZIP18-sMHT(114-230)

Expression

Bacterial

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDH006

Plasmid

#157641

Purpose

sMHT-113 fused SYNZIP18 only

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)
SYNZIP18-sMHT(114-23)

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDH008

Plasmid

#157643

Purpose

sMHT-113 fused to CheY only

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)
CheY-sMHT(1-114)

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEMF036

Plasmid

#157633

Purpose

sMHT-129

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-129)-SYNZIP17
SYNZIP18-sMHT(130-230)

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEMF047

Plasmid

#157636

Purpose

sMHT-113 F1 only

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Insert

sMHT(1-113)-SYNZIP17

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEMF044

Plasmid

#157637

Purpose

sMHT-113 F2 only

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Insert

SYNZIP18-sMHT(114-230)

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDH001

Plasmid

#157639

Purpose

Batis maritima MHT

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Insert

MHT

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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PD19

Plasmid

#164528

Purpose

Expresses Flag-Ub noK

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Ubiquitin noK

Use

Lentiviral

Tags

FLAG and Ub noK

Mutation

All seven Lysines of Ubiquitin changed to Arginine

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1245

Plasmid

#155139

Purpose

Expresses MlaF in E. coli

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

6xHis-TEV-mlaF

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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