We narrowed to 4,396 results for: erf

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pEGFP GLTP

Plasmid

#170746

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

Insert

GLTP (GLTP Human)

Tags

EGFP

Expression

Mammalian

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP

Plasmid

#196988

Purpose

Derived from pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP, custom sgRNA cloning vector

Depositor

Elizabeth Kirby

Article

Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

Insert

Nestin-dCas9-KRAB-T2a-GFP

Use

CRISPR and Lentiviral

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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BAS286 C terminal SF-GFP hygBR

Plasmid

#74081

Purpose

S.pombe recoded superfolder GFP in universal C-terminal tagging plasmid, hygB resistanc

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

super-folder GFP

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
pCas9-beta

Plasmid

#169240

Purpose

Cas9 and beta expression plasmid for performing gene knockouts via oligo recombineering

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Inserts

cas9
beta

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

Plasmid

#167898

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPRai

Plasmid

#189938

Purpose

Expresses CRISPRai cassette under EF1a promoter

Depositor

Yu-Chen Hu

Article

Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7

Insert

CRISPRai cassette

Expression

Mammalian

Promoter

CMV enhancer-rat EF1 alpha

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHW450

Plasmid

#198831

Purpose

Voltage-sensitive fluorescent protein.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::VSFP-Butterfly-1-2::let-858 3'UTR

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CibN-dCas9

Plasmid

#60551

Purpose

Expresses CibN-dCas9 in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

CibN-dCas9

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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Luciferase shRNA

Plasmid

#225343

Purpose

shRNAmir backbone for RNA interference, with YFP

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

Luciferase shRNA (LOC116160065 Mouse)

Use

AAV and RNAi

Expression

Mammalian

Promoter

CBA

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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HiFi dCas9 KRAB

Plasmid

#188500

Purpose

Expresses FLAG tagged HiFi dCas9 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/R691A/H840A

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMD19T-psba1-TetR-PL22-dCas9-SpR

Plasmid

#73223

Purpose

Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

Depositor

Paul Hudson

Article

Yao et al ACS Synth Biol. 2015 Dec 28.

Insert

dCas9 from S. pyogenes

Tags

c-myc

Expression

Bacterial

Mutation

Silent mutation at bp 1341 A->C to remove an E…

Promoter

PL22

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pJMP1223

Plasmid

#119261

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1335

Plasmid

#119269

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL Hygro-NFLAG TRF1 deltaA

Plasmid

#16278

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Deleted acidic domain (aa1-66)

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pWZL Hygro-NFLAG TRF1 deltaM

Plasmid

#16281

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Deleted myb domain (aa379-439)

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-dCas9-CibN

Plasmid

#60552

Purpose

Expresses dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pMCSG28-SH3-588

Plasmid

#185920

Purpose

Expresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferase

Depositor

Brian Kuhlman

Article

Park et al Nat Chem Biol. 2023 Apr;19(4):460-467. d

Insert

SH3-588

Tags

6x HIS tag

Expression

Bacterial

Mutation

Designed enzyme with novel interface between huma…

Promoter

T7

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pJMP1363

Plasmid

#119279

Purpose

for stabilizing ICE in the presence of rapI expression

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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POLArIS_NbVim-cp10GFP

Plasmid

#171020

Purpose

Expresses NbVim-cp10GFP#2 in mammalian cells

Depositor

Sumio Terada

Article

Nakai et al Biochem Biophys Res Commun. 2021 Jun 2;5

Insert

anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)

Tags

cp10GFP

Expression

Bacterial and Mammalian

Promoter

CMV promoter

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD_A0402

Plasmid

#91009

Purpose

Module A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

AtUbi10

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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