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pGEX Nap4-SH2

Plasmid

#46456

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Nap4 (SOCS7 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 395-514

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Nsp1-SH2

Plasmid

#46459

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Nsp1 (SH2D3A Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 3-119

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf226

Plasmid

#12914

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf226

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
pSMP-NR2F1_3

Plasmid

#36364

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

NR2F1 (NR2F1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA mouse NHERF-1 (1-325)

Plasmid

#32706

Depositor

Edward Weinman

Article

Weinman et al Am J Physiol Renal Physiol. 2001 Aug;281

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (Nherf1 Mouse)

Tags

none

Expression

Mammalian

Mutation

Deleted last 30 AA putative Ezrin binding site

Promoter

CMV

Available Since

Feb. 28, 2012

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf110

Plasmid

#12798

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf110

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pMS1251

Plasmid

#80163

Purpose

pET-Duet for bacterial expression of Ald-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme

Depositor

Marcelo Sousa

Article

Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/

Insert

HisSUMO-Ald-4xNuG2-Ald

Tags

HisSUMO

Promoter

T7

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSMP-NR2F1_1

Plasmid

#36362

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

NR2F1 (NR2F1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pET16b-Trx80

Plasmid

#36945

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Trx80 (truncated Trx) (TXN Human)

Tags

His tag

Expression

Bacterial

Mutation

premature stop inserted after K80 in Trx1 gene

Promoter

T7

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf67

Plasmid

#12755

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf67

Expression

Mammalian

Available Since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf148

Plasmid

#12836

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf148

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf72

Plasmid

#12760

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

A34R

Expression

Mammalian

Available Since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf263

Plasmid

#12951

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf263

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf82

Plasmid

#12770

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf82

Expression

Mammalian

Available Since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf152

Plasmid

#12840

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf152

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pRubiG-T2A-AKT3(S472A)-T2A-Cre

Plasmid

#189009

Purpose

Retroviral vector with a ubiquitin promoter expressing GFP, human AKT3 transcript variant 1 ORF with a phosphodeficient mutation at Serine 472, and Cre recombinase, separated by T2A motifs.

Depositor

Bryan Luikart

Insert

AKT3 (AKT3 Human)

Use

Retroviral

Mutation

Serine at 472 mutated to Alanine

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pRubiG-T2A-AKT3(S472D)-T2A-Cre

Plasmid

#189010

Purpose

Retroviral vector with a ubiquitin promoter expressing GFP, human AKT3 transcript variant 1 ORF with a phosphomimetic mutation at Serine 472, and Cre recombinase, separated by T2A motifs.

Depositor

Bryan Luikart

Insert

AKT3 (AKT3 Human)

Use

Retroviral

Mutation

Serine at 472 mutated to Aspartic Acid

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pRubiG-T2A-AKT1(S473A)-T2A-Cre

Plasmid

#189012

Purpose

Retroviral vector with a ubiquitin promoter expressing GFP, human AKT1 ORF with a phosphodeficient mutation at Serine 473, and Cre recombinase, separated by T2A motifs.

Depositor

Bryan Luikart

Insert

AKT1 (AKT1 Human)

Use

Retroviral

Mutation

Serine at 473 mutated to Alanine

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pRubiG-T2A-AKT1(S473D)-T2A-Cre

Plasmid

#189013

Purpose

Retroviral vector with a ubiquitin promoter expressing GFP, human AKT1 ORF with a phosphomimetic mutation at Serine 473, and Cre recombinase, separated by T2A motifs.

Depositor

Bryan Luikart

Insert

AKT1 (AKT1 Human)

Use

Retroviral

Mutation

Serine at 473 mutated to Aspartic Acid

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-sgStuffer-GFP-Puro

Plasmid

#208349

Purpose

Lentivirus sgRNA backbone with GFP and PuroR. Compatible with golden gate cloning (Esp3i/BsmBI)

Depositor

Peter S Nelson

Article

Frank et al eLife 2025

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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