We narrowed to 73,945 results for: LAS

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MSCV-opEBNA2-IRES-mCherry

Plasmid

#220355

Purpose

Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression)

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Expression

Mammalian

Mutation

codon-optimised for expression in mammalian cells

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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NKP38 GFP (1009)

Plasmid

#62021

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

Insert

AYTL1 (LPCAT2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pKS004 - pCAGGS-3XFLAG-CTCF-eGFP

Plasmid

#156438

Purpose

For transient expression of mouse CTCF tagged with eGFP

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse CTCF (Ctcf Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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6691 Bicistronic_ires_puro

Plasmid

#64335

Purpose

This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Type

Empty backbone

Use

Retroviral

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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MBP-CHC(1-1074)-His6

Plasmid

#100748

Purpose

Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CLTC (CLTC Human)

Tags

His and MBP

Expression

Bacterial

Mutation

Residues 1-1074

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-nsp2

Plasmid

#168948

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-nsp2 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry

Plasmid

#35510

Purpose

AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123T and T159C

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA pDEST40 Cas9-POLD3

Plasmid

#183194

Purpose

Expression clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

POLD3

Use

CRISPR

Tags

Cas9WT

Expression

Mammalian

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DHFR.cHSF1

Plasmid

#134739

Purpose

Lentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

Insert

DHFR.cHSF1 (HSF1 Human)

Use

Lentiviral

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202

Promoter

CMV

Available Since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pET28:LanIII

Plasmid

#225075

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanIII (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanIII)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanIII-43

Plasmid

#225077

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanIII-43 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanIII-43)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII

Plasmid

#225066

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-229E-Spike

Plasmid

#168971

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-Spike (S )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-2-nsp16

Plasmid

#168876

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-2-nsp16 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMT-sox10-cytoBirA-2A-mCherry_Ras

Plasmid

#80062

Purpose

Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Sox10 promoter

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-eArch 3.0-EYFP

Plasmid

#35516

Purpose

AAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

Arch3.0-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Enhanced trafficking signal and ER export signal …

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pmCherry-CRY2-CreN

Plasmid

#26888

Purpose

Expresses N-terminal fragment of Cre fused to CRY2 for blue light-induced activation of DNA recombination when used with CIBN-CreC. Also expresses mCherry.

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2-CreN (cre, CRY2 Mustard Weed)

Expression

Mammalian

Mutation

aa 19-104 of Cre

Promoter

CMV

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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p11d-tRPA (MSW#111)

Plasmid

#208066

Purpose

expression of human Replication Protein A in E. coli

Depositor

Marc Wold

Article

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Insert

RPA1, RPA2, RPA3 (RPA1, RPA3, RPA2 Human)

Expression

Bacterial

Mutation

RPA32 has a point mutation Thr88(ACC) -> Ile(A…

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_MERS-CoV-nsp4

Plasmid

#168817

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-nsp4 (orf1ab )

Use

Gateway-compatible entry vector

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223 MERS-CoV NSP6_stop

Plasmid

#168633

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP6 (orf1ab )

Use

Other

Available Since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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