We narrowed to 10,318 results for: plasmids 101

1. pCRISPaint-mNeon-F-mgp100-PuroR [M1G]

   Plasmid

   #171802

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-FLAG-mgp100-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Spike Display_Part 2 DO

   Plasmid

   #172722

   Purpose

   Encodes a sfGFP dropout expression cassette in place of Part 2 of HexaPro-D614G spike to be used in the Spike Display system

   Depositor

   IIya Finkelstein

   Article

   Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

   Insert

   SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

   Expression

   Mammalian

   Mutation

   Ectodomain only (AAs 1-1208); 682-685 (furin site…

   Promoter

   CMV

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

   Plasmid

   #171801

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-FLAG-hgp100-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPaint-mScarlet-F-Ova-PuroR [M1G]

   Plasmid

   #171813

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-F-Ova-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV hSyn INTRON mScarlet-STIM2 WPRE

   Plasmid

   #236236

   Purpose

   AAV expression of human STIM2 internally tagged with mScarlet-I

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   mScarletI-STIM2 (STIM2 Human)
   

   Use

   AAV

   Tags

   mScarletI in position 29

   Expression

   Mutation

   Signal peptide from STIM1 and residues 15-746 of …

   Promoter

   human Synapsin 1

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV hSyn INTRON HaloTag-STIM2 WPRE

   Plasmid

   #236235

   Purpose

   AAV expression of human STIM2 internally tagged with HaloTag

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   HaloTag-STIM2 (STIM2 Human)
   

   Use

   AAV

   Tags

   HaloTag in position 29

   Expression

   Mutation

   Signal peptide from STIM1 and residues 15-746 of …

   Promoter

   human Synapsin 1

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTwist-SARS-CoV-2 Spike-deltaC18 B.1.617v5 (B.1.617.3)

   Plasmid

   #212540

   Purpose

   Encodes SARS-CoV-2 spike T19R,G142D,E156-F157 deletion,R158G,N450R,E484Q,D614G,P681R,D950N for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike T19R,G142D,E156-F157 deletion,R158G,N450R,E484Q,D614G,P681R,D950N (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T19R,G142D,E156-F157 deletion,R158G,N450R,E484Q,D…

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTwist-SARS-CoV-2 Spike-deltaC18 BA.4

   Plasmid

   #212519

   Purpose

   Encodes SARS-CoV-2 variant BA.4 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike BA.4 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T19I ,deltaL24P25P26 ,A27S ,delH69-V70 ,G142D ,V2…

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTwist-SARS-CoV-2 Spike-deltaC18 Q493R (CGA) G496S (TCA) Q498R (CGA) N501Y (TAT) Y505H (CAT)

   Plasmid

   #212477

   Purpose

   Encodes SARS-CoV-2 spike Q493R, G496S, Q498R, N501Y, Y505H for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike Q493R, G496S, Q498R, N501Y, Y505H (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q493R, G496S, Q498R, N501Y, Y505H

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTwist-SARS-CoV-2 Spike-deltaC18 AY.1

    Plasmid

    #212433

    Purpose

    Encodes SARS-CoV-2 spike T19R ,T95I ,G142D ,deltaE156-F157+R158G ,W258L ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike T19R ,T95I ,G142D ,deltaE156-F157+R158G ,W258L ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T19R ,T95I ,G142D ,deltaE156-F157+R158G ,W258L ,K…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTwist-SARS-CoV-2 Spike-deltaC18 AY.2

    Plasmid

    #212434

    Purpose

    Encodes SARS-CoV-2 spike T19R ,V70F ,G142D ,deltaE156-F157+R158G ,A222V ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike T19R ,V70F ,G142D ,deltaE156-F157+R158G ,A222V ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T19R ,V70F ,G142D ,deltaE156-F157+R158G ,A222V ,K…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTwist-SARS-CoV-2 Spike-deltaC18 B.1.617v2 (B.1.617.1 v2)

    Plasmid

    #212429

    Purpose

    Encodes SARS-CoV-2 spike T95I,G142D,E154K,E156-F157 deletion,R158G,L452R,E484Q,D614G,P681R,Q1071H for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike T95I,G142D,E154K,E156-F157 deletion,R158G,L452R,E484Q,D614G,P681R,Q1071H (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T95I,G142D,E154K,E156-F157 deletion,R158G,L452R,E…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTwist-SARS-CoV-2 Spike-deltaC18 SAK006879 VOC-501Y.V2 (B.1.351v5)

    Plasmid

    #212391

    Purpose

    Encodes SARS-CoV-2 spike D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,A701V for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,A701V (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTwist-SARS-CoV-2 Spike-deltaC18 SAK007134 VOC-501Y.V2 (B.1.351v6)

    Plasmid

    #212393

    Purpose

    Encodes SARS-CoV-2 spike A27S,D80A,D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,A701V for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike A27S,D80A,D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,A701V (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A27S,D80A,D215G,L242-L244 deletion,K417N,E484K,N5…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTwist-SARS-CoV-2 Spike-deltaC18 SA190 VOC-501Y.V2 (B.1.351v4)

    Plasmid

    #212389

    Purpose

    Encodes SARS-CoV-2 spike D80A,D215G,L242-L244 deletion,E484K,N501Y,D614G,A701V for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike D80A,D215G,L242-L244 deletion,E484K,N501Y,D614G,A701V (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D80A,D215G,L242-L244 deletion,E484K,N501Y,D614G,A…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTwist-SARS-CoV-2 Spike-deltaC18 VOC-501Y.V2 (B.1.351) - All Mutations

    Plasmid

    #212381

    Purpose

    Encodes SARS-CoV-2 spike L18F,D80A,D215G,L242H,A243I,Q414N,E484K,N501Y,D614G,A701V for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike L18F,D80A,D215G,L242H,A243I,Q414N,E484K,N501Y,D614G,A701V (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L18F,D80A,D215G,L242H,A243I,Q414N,E484K,N501Y,D61…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pαH-S-GSAS-2P-N317P

    Plasmid

    #196378

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein with mutation N317P with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

    Depositor

    Priyamvada Acharya

    Article

    Manrique et al iScience. 2023 Jan 20;26(1):105855. doi:

    Insert

    S-GSAS-2P-N317P (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV 3C cleavage site (before tags) C terminal on …

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

18. Spike Display_Part 1 DO

    Plasmid

    #172721

    Purpose

    Encodes a sfGFP dropout expression cassette in place of Part 1 of HexaPro-D614G spike to be used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Spike Display_Part-5 DO

    Plasmid

    #172725

    Purpose

    Encodes a sfGFP dropout expression cassette in place of Part 5 of HexaPro-D614G spike to be used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Spike Display_Part 3 DO

    Plasmid

    #172723

    Purpose

    Encodes a sfGFP dropout expression cassette in place of Part 3 of HexaPro-D614G spike to be used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge