We narrowed to 13,646 results for: NTS

1. pGWB510

   Plasmid

   #74852

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAGM55273

   Plasmid

   #153211

   Purpose

   Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EGFP-E-Syt3

   Plasmid

   #66832

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt3 (ESYT3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDGE347

   Plasmid

   #153228

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGWB413

   Plasmid

   #74807

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDGE334

   Plasmid

   #153243

   Purpose

   M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDGE337

   Plasmid

   #153246

   Purpose

   M4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ArcLight-Q239

   Plasmid

   #36856

   Depositor

   Vincent Pieribone

   Article

   Jin et al Neuron. 2012 Sep 6;75(5):779-85.

   Insert

   fluorescent protein voltage sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Ci-VSP contains R217Q mutation; super ecliptic pH…

   Promoter

   simian CMV

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2/CMV-Luc/PGK-SB100X

   Plasmid

   #192869

   Purpose

   Carries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.

   Depositor

   David Largaespada, Maria Castro

   Article

   Calinescu et al J Vis Exp. 2015 Feb 22;(96). doi: 10.379

   Inserts

   luc
   SB100X
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSG5-ERRbeta2

    Plasmid

    #52186

    Purpose

    Expresses human ERRbeta2 splice variant in mammalian cells

    Depositor

    Rebecca Riggins

    Article

    Heckler et al Cell Cycle. 2014 Dec 12:0.

    Insert

    Estrogen-related receptor beta (ESRRB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type sequence; codon optimized to exclude Ec…

    Promoter

    SV40

    Available since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO.1 shSV40 neo

    Plasmid

    #158646

    Purpose

    Lentiviral shRNA vector targeting simian virus 40 T antigen (SV40Tag)

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    SV40Tag (SV40gp6 Simian virus 40)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. hSyn1-2xNLS-mTurquoise2

    Plasmid

    #118025

    Purpose

    An AAV genome that expresses nuclear localized mTurquoise2 from the hSyn1 promoter

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    2xNLS-mTurquoise2
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a_Pro-IL-1α

    Plasmid

    #214341

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL1A (IL1A Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. piggyFlex

    Plasmid

    #218234

    Purpose

    A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.

    Depositor

    Jay Shendure

    Article

    Chardon et al bioRxiv [Preprint]. 2023 Mar 28:2023.03.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGWB553

    Plasmid

    #74883

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-SlugABE-NNG-P2A-puro

    Plasmid

    #214354

    Purpose

    Expresses SlugABE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugABE-NNG and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGWB519

    Plasmid

    #74861

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKLV-EF1amCherry-2A-OsTIR1-W

    Plasmid

    #159296

    Purpose

    Lentiviral vector expressing mCherry and OsTIR1 driven by human EF1a promoter for the auxin-inducible degron (AID) system

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    OsTIR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human EF1a promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-noPS

    Plasmid

    #177943

    Purpose

    Transient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAT9679-sgRNA-BFP

    Plasmid

    #162988

    Purpose

    sgRNA cloning plasmid

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    exchangeable cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPSup_35Senh(fwd)_35Spr+synJ

    Plasmid

    #149422

    Purpose

    Plasmid for STARR-seq in tobacco leaves. The GFP reporter gene is under the control of a 35S minimal promoter and an upstream 35S core enhancer.

    Depositor

    Stan Fields

    Article

    Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

    Insert

    35S core enhancer
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-BE4-PpAPOBEC1 R33A

    Plasmid

    #138345

    Purpose

    Mammalian expression plasmid for BE4-PpABOBEC1 R33A

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-PpAPOBEC1 R33A
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Expression

    Mutation

    Change of Arg 33 to Ala.

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mApple-Annexin(A4)-12

    Plasmid

    #54867

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Annexin (A4) (ANXA4 Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pBiFC-bJunVN173

    Plasmid

    #22012

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    bJunVN173 (Jun Aequorea Victoria, Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    c-Jun(257-338)-Venus(1-172)

    Promoter

    Available since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TDP43 NOTAG1

    Plasmid

    #28206

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Full length

    Promoter

    Available since

    July 1, 2011

    Availability

    Academic Institutions and Nonprofits only

26. pGWB535

    Plasmid

    #74873

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAT9675-CBE

    Plasmid

    #163007

    Purpose

    plasmid expressing CBE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    CBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAT9748-dCBE

    Plasmid

    #163008

    Purpose

    plasmid expressing CBE with dCas9

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    dCBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMTMVi-M

    Plasmid

    #118757

    Purpose

    Intermediate plasmid to build tobacco mosaic virus recombinant clones that move systemically throughout the plant.

    Depositor

    Jose-Antonio Daros

    Article

    Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

    Insert

    Tobacco mosaic virus cDNA fragment with a cloning polylinker
    

    Use

    Intermediate to build tobacco mosaic virus recomb…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGWB518

    Plasmid

    #74860

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 4xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge