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pX459-sg-CMV162

Plasmid

#179917

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-mGreenLantern332

Plasmid

#179914

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV_Hyg_FLAG-BirA-eGFP-NLS2

Plasmid

#170917

Purpose

Expresses FLAG-BirA-eGFP-NLS2 to be used as control in BioID or FLAG pull down

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

FLAG-BirA-eGFP-NLS2

Tags

BirA-FLAG

Expression

Mammalian

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_HR-BirA-FLAG_Ago2

Plasmid

#170918

Purpose

Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago2

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

BirA-FLAG

Expression

Mammalian

Promoter

lac

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_HR-BirA-FLAG_Ago1

Plasmid

#170919

Purpose

Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago1

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

BirA-FLAG

Expression

Mammalian

Promoter

lac

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-zfCSF3b

Plasmid

#168202

Purpose

Express zebrafish Csf3b, mRNA synthesis

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

Insert

Csf3b (csf3b Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B K88E

Plasmid

#175028

Purpose

Expresses WIPI2B (K88E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (K88E) (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Lysine 88 to Glutamate (AAG to GAG)

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B I92E

Plasmid

#175029

Purpose

Expresses WIPI2B (I92E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (I92E) (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Isoleucine 92 to Glutamate (ATC to GAA)

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B C93E

Plasmid

#175030

Purpose

Expresses WIPI2B (C93E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (C93E) (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Cysteine 93 to Glutamate (TGC to GAG)

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B K128E

Plasmid

#175031

Purpose

Expresses WIPI2B (K128E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (K128E) (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Lysine 128 to Glutamate (AAG to GAG)

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMP71-mGFP-LLO-hPMEL

Plasmid

#174607

Purpose

expresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the human hPMEL epitope

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

expresses palmitoylated GFP w/C-term fusion of the LLO190 epitope and human hPMEL epitope

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (E60amb)-His

Plasmid

#168043

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Glu 60 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK129-stm2585_Y167F

Plasmid

#174402

Purpose

Bacterial expression of sarA with its STAT3-binding YxxQ motif mutated to FxxQ

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Expression

Bacterial

Mutation

sarA with Y167F mutation

Promoter

Endogenous

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-zfCSF3a

Plasmid

#168201

Purpose

Express zebrafish Csf3a, mRNA synthesis

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

Insert

Csf3a (csf3a Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Mutation

Deletion of A27

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (E143amb)-His

Plasmid

#168046

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Glu 143 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (S87amb)-His

Plasmid

#168044

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Ser 87 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (N116amb)-His

Plasmid

#168045

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Asn 116 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (N21amb)-His

Plasmid

#168041

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Asn 21 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Fgβ-anticalin-His-ybbr

Plasmid

#168040

Purpose

Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag, Fgβ peptide, and ybbr tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (I55amb)-His

Plasmid

#168042

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Ile 55 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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