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Plasmid#108363PurposeA nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.DepositorInsertloxP-natMX6-CgMET3prom-cre-loxP
UseGene disruption marker for candida glabrataTagsExpressionMutationPromoterC. glabrata MET3Available sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
VEGF binding scFv G6
Plasmid#111716PurposeYeast Surface Display of G6 as a reference starting point for designDepositorInsertG6
UseTagscmyc tagExpressionYeastMutationPromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor
Plasmid#211904PurposeVector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.DepositorInsertCD19-CAR-28z-2A-EGFP
UseAAVTagsExpressionMammalianMutationPromoterhPGK1Available sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-CHIKV-nsp3-c3xFLAG
Plasmid#215696PurposeProduces the Chikungunya virus nonstructural protein 3 with a c-terminal 3xFLAG tagDepositorInsertCHIKV nsp3
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE80L-SH3
Plasmid#160331PurposeThis plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its thermostability and quantum yield is improved compared to wild type.DepositorInsertSH3
UseTags6xHis tagExpressionBacterialMutationPromoterT5Available sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA
Plasmid#156440PurposeFor transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2DepositorInsertmouse SA1 (Stag1 Mouse)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-mCherry-SPASTIN
Plasmid#180647PurposeLentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.DepositorInsertSPASTIN
UseLentiviralTagsmCherryExpressionMammalianMutationStarts at M87, has siRNA resistance to GAACAGUGUG…PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
JF001A
Plasmid#53116Purposeexpresses alpha hemolysin under the control of theophylline riboswitchDepositorInserttheophylline riboswitch and alpha hemolysin
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-PAL1C
Plasmid#196685PurposeRep/Cap plasmid for the production of PAL1C, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationPTQGTLR insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
145-pGL3ctrl-3' UTR
Plasmid#28175DepositorInsertLuciferase flanked by tp53 UTRs (TP53 Human)
UseLuciferaseTagsExpressionMammalianMutationNote that insert length is based on sum of the 2 …PromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Ha-Brca1 S1423A
Plasmid#32533DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHAExpressionMammalianMutationS1423APromoterCMV/rabbit B-globinAvailable sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pK1E-RNAP
Plasmid#48144PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylADepositorInsertrna polymerase K1E
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
hCCR5-LSSmOrange
Plasmid#110194PurposeEncodes for human CCR5 coding sequence tagged with the LSS-mOrange FPDepositorInserthCCR5 (CCR5 Human)
UseTagsLSSmOrangeExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOTTC841 - pAAV minP secNLuc
Plasmid#82498PurposeLongitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivoDepositorInsertsecNLuc
UseAAVTagsExpressionMammalianMutationPromoterc-Fos minimal promoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_26H
Plasmid#91150PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:barDepositorInsertEngineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
HOXB8 (murine) HIS-tag pET
Plasmid#8540DepositorInsertHOXB8 (Hoxb8 Mouse)
UseTagsHis and T7ExpressionBacterialMutationstarts at amino acid #2 (Ser)PromoterAvailable sinceAug. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-EPA-DNNNS-DQNRT
Plasmid#128404PurposePeriplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coliDepositorInsertExotoxin A (toxA P. aeruginosa)
UseTags6xHis tag and DsbA signal sequence for periplasmi…ExpressionBacterialMutation242DNNNS246 and 384DQNRT388 engineered glycosylat…PromotertrcAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Ha-Brca1 S1387A
Plasmid#32532DepositorInsertBrca1 (BRCA1 Human)
UseTagsHAExpressionMammalianMutationS1387APromoterCMV/Rabbit B-GlobinAvailable sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMP71-mIL15-IL12rb1-sushi-il12rb2
Plasmid#174606PurposeExpresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tagsDepositorInsertExpresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
iRFP713-N2-Frankenbody-Flag
Plasmid#175291PurposeVisualize endogeneous loci coupled with ZF probes harboring 10xFLAG tagDepositorInsertanti-FLAG frankenbody fused with iRFP713
UseTagsiRFP713ExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits