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pLentiCRISPR V2 Neo sgITGA5_1

Plasmid

#233251

Purpose

Knock out of murine ITGA5

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

ITGA5 (Itga5 Mouse)

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pMVP-SEC24C-G220C

Plasmid

#231560

Purpose

pMVP expression vector for human SEC24C (G220C mutation, closed, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)

Depositor

Rizwan Haq

Article

Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

Insert

SEC24C (G220C) (SEC24C Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

G220C, silent PAM mutations (G67 (GGG>GGA) and…

Promoter

CMV

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMVP-SEC24C-S107F

Plasmid

#231561

Purpose

pMVP expression vector for human SEC24C (S107F mutation, closesd, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)

Depositor

Rizwan Haq

Article

Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

Insert

SEC24C (S107F) (SEC24C Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S107F, silent PAM mutations (G67 (GGG>GGA) and…

Promoter

CMV

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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HA-MAP4K4-10A

Plasmid

#229403

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MAP4K4 (MAP4K4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

MARK2 phosphosites (S523,S536,S547,S643,T684,S726…

Promoter

EFS

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MAP4K4-10D

Plasmid

#229404

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MAP4K4 (MAP4K4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

MARK2 phosphosites (S523,S536,S547,S643,T684,S726…

Promoter

EFS

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 Q280* K418* ectodomain

Plasmid

#221399

Purpose

Mammalian expression of human integrin beta1 Q280* K418* ectodomain

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* K418* ectodomain (ITGB1 Human)

Tags

AVI tag, HRV3C cleavage site, basic coil, HA tag,…

Expression

Mammalian

Mutation

codon optimized for human mature _1 residues Q1 t…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 N391* N562* ectodomain

Plasmid

#221400

Purpose

Mammalian expression of human integrin beta1 N391* N562* ectodomain

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* N562* ectodomain (ITGB1 Human)

Tags

AVI tag, HRV3C cleavage site, basic coil, HA tag,…

Expression

Mammalian

Mutation

codon optimized for human mature _1 residues Q1 t…

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROD1L

Plasmid

#215043

Purpose

Mammalian expression of human TWIST1 (with fragment of loop from NEUROD1)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Tags

V5

Expression

Mammalian

Mutation

Left portion of TWIST1 bHLH loop (aa137-139, TLP)…

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROD1R

Plasmid

#215044

Purpose

Mammalian expression of human TWIST1 (with fragment of loop from NEUROD1)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Tags

V5

Expression

Mammalian

Mutation

Right portion of TWIST1 bHLH loop (aa139-141, PSD…

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet_Seq1.3

Plasmid

#206137

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1 (Col1a1 Mouse)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3076

Plasmid

#144552

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

GTF2A2 (GTF2A2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC1664 - pAAV SYN1 mGas6(delta)-Myc-DDK

Plasmid

#202541

Purpose

An adeno-associated viral vector expressing murine Gas6 with deletion of (F50-E275) fused Myc and DDK epitopes from a synapsin promoter

Depositor

Christopher Richie

Article

Owlett et al J Neuroinflammation. 2022 Feb 7;19(1):38

Insert

Gas6 (Gas6 Mouse)

Use

AAV

Tags

Myc-DDK

Mutation

Deletion of F50-E275

Promoter

SYN1

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169_X

Plasmid

#147983

Purpose

Insect Expression of DmNot1_1-1717-del1156-1169

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-1717-del1156-1169 (Not1 Fly)

Expression

Insect

Mutation

6 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169-R1197AN1203AF1204A_X

Plasmid

#147984

Purpose

Insect Expression of DmNot1_1-1717-del1156-1169-R1197AN1203AF1204A

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-1717-del1156-1169-R1197AN1203AF1204A (Not1 Fly)

Expression

Insect

Mutation

6 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA_X

Plasmid

#147985

Purpose

Insect Expression of DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA (Not1 Fly)

Expression

Insect

Mutation

6 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1945-K1537SG1562EQ1658A-dsRNAres_X

Plasmid

#147990

Purpose

Insect Expression of DmNot1_1-1945-K1537SG1562EQ1658A-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-1945-K1537SG1562EQ1658A-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

8 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1-del1156-1169-dsRNAres_X

Plasmid

#147991

Purpose

Insect Expression of DmNot1-del1156-1169-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-del1156-1169-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W

Plasmid

#147875

Purpose

Insect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-del1156-1169-RNFtoAAA-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W

Plasmid

#147859

Purpose

Insect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-del1156-1169-RNFtoAAA-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-sgFam135b#2/Cre

Plasmid

#193214

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Fam135b gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgFam135b#1 (Fam135b Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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