Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 5,457 results for: golden gate

Clear keywords
Showing: 3661 - 3680 of 5457 results

  1. pYSD892

    Plasmid
    #253018
    Purpose
    A MoClo YTK Type 3 part GFP dropout vector to assemble a signal peptide and protein of interest as N-terminal fusion with 2xFLAG-Aga1 del N181aa anchor in a HIS3, CEN6/ARS4 yeast expression plasmid
    Depositor
    Paul Young
    Publication
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    GFP
    Expression
    Yeast
    Available since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253018-plasmid-map-sequence-id-508727

    Enlarge

  2. pYSD891

    Plasmid
    #253017
    Purpose
    A MoClo YTK Type 3 part GFP dropout vector to assemble a signal peptide and protein of interest as N-terminal fusion with 2xFLAG-649 stalk display anchor in a LEU2, CEN6/ARS4 yeast expression plasmid
    Depositor
    Paul Young
    Publication
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    GFP
    Expression
    Yeast
    Available since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253017-plasmid-map-sequence-id-508726

    Enlarge

  3. pYSD728

    Plasmid
    #253050
    Purpose
    Encodes OST1 Sig Pep, LC Cutinase-WT, FLAG-tagged SED1 surface display anchor in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker
    Depositor
    Paul Young
    Publication
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Leaf compost cutinase
    Expression
    Yeast
    Available since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253050-plasmid-map-sequence-id-508705

    Enlarge

  4. pYSD726

    Plasmid
    #253049
    Purpose
    Encodes OST1 Sig Pep, LC Cutinase-ICCG variant, FLAG-tagged SED1 surface display anchor in a yeast expression vector with pTEF1 promoter, tTDH1 term, LEU2 selectable marker and CEN/ARS origin
    Depositor
    Paul Young
    Publication
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Leaf compost cutinase
    Expression
    Yeast
    Mutation
    Y127G, D238C, F243I, S283C
    Available since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253049-plasmid-map-sequence-id-508689

    Enlarge

  5. pYSD078

    Plasmid
    #252975
    Purpose
    Encodes a MoClo Yeast Toolkit (YTK) Type 3 GFP dropout part that can be used to make a YTK GFP dropout vector containing all part types except Type 3
    Depositor
    Paul Young
    Publication
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Protein A
    Expression
    Bacterial
    Available since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    252975-plasmid-map-sequence-id-508688

    Enlarge

  6. pYSD890

    Plasmid
    #253016
    Purpose
    Encodes S. cerevisiae Met15 as a Type 6 part to be used in the MoClo yeast toolkit (YTK) and yeast secretion/display (YSD2.0) extension of the MoClo YTK
    Depositor
    Paul Young
    Publication
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    MET15
    Expression
    Bacterial
    Available since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253016-plasmid-map-sequence-id-508685

    Enlarge

  7. pYSD086

    Plasmid
    #252976
    Purpose
    Encodes FLAG-tagged S. cerevisiae Sed1p display anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Publication
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    FLAG-tagged Sed1
    Expression
    Bacterial
    Available since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    252976-plasmid-map-sequence-id-508653

    Enlarge

  10. M_5HN

    Plasmid
    #249755
    Purpose
    Minimal synthetic construct for assessing speckle localization containing a 5' splice site and an HNRNP motif-rich region
    Depositor
    Oded Regev
    Publication
    Wen et al bioRxiv [Preprint]. 2025 May 28:2025.05.
    Insert
    M_5HN
    Expression
    Mammalian
    Promoter
    TRE+CMV
    Available since
    April 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    249755-plasmid-map-sequence-id-502717

    Enlarge

  15. pEB1_6b_CusR-VIII-ctrl

    Plasmid
    #236218
    Purpose
    Control for expression system in pEB2_4: Plasmid expressing mCherry under 8xCusR_BSsENO1cp with homologous regions for genome integration into HO locus.
    Depositor
    Yvonne Nygård
    Publication
    Blick et al Synth Biol (Oxf). 2025 Dec 4;10(1):ysaf0
    Insert
    mCherry, SSA1 terminator
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    8xCusR_BSsENO1cp
    Available since
    April 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    236218-plasmid-map-sequence-id-508219

    Enlarge

  17. M_B2

    Plasmid
    #249764
    Purpose
    Minimal synthetic construct for assessing speckle localization, part of B trajectory series (starting with M_3HN5 and ending in M_3SR5)
    Depositor
    Oded Regev
    Publication
    Wen et al bioRxiv [Preprint]. 2025 May 28:2025.05.
    Insert
    M_B2
    Expression
    Mammalian
    Promoter
    TRE+CMV
    Available since
    April 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    249764-plasmid-map-sequence-id-508160

    Enlarge

  18. M_B3

    Plasmid
    #249765
    Purpose
    Minimal synthetic construct for assessing speckle localization, part of B trajectory series (starting with M_3HN5 and ending in M_3SR5)
    Depositor
    Oded Regev
    Publication
    Wen et al bioRxiv [Preprint]. 2025 May 28:2025.05.
    Insert
    M_B3
    Expression
    Mammalian
    Promoter
    TRE+CMV
    Available since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    249765-plasmid-map-sequence-id-508127

    Enlarge

  19. M_A6

    Plasmid
    #249762
    Purpose
    Minimal synthetic construct for assessing speckle localization, part of A trajectory series (starting with M_3HN5 and ending in M_3SR5)
    Depositor
    Oded Regev
    Publication
    Wen et al bioRxiv [Preprint]. 2025 May 28:2025.05.
    Insert
    M_A6
    Expression
    Mammalian
    Promoter
    TRE+CMV
    Available since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    249762-plasmid-map-sequence-id-508124

    Enlarge

Showing: 3661 - 3680 of 5457 results