We narrowed to 3,807 results for: golden gate

1. pNK5785

   Plasmid

   #219753

   Purpose

   pGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pGAP - nnLuz_v4 - tAOX
   

   Use

   Luciferase

   Expression

   Yeast

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBFC0993

   Plasmid

   #231993

   Purpose

   CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer

   Depositor

   Brady Cress

   Article

   Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

   Inserts

   dRfxCas13d
   crRNA with negative control (RFP-targeting) spacer
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119 and pTet

   Available Since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBFC1171

   Plasmid

   #231994

   Purpose

   CRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning site

   Depositor

   Brady Cress

   Article

   Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

   Insert

   crRNA of dRfxCas13d
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   pJEx (Jungle Express)

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMB.BIG1a.PRC2

   Plasmid

   #234581

   Purpose

   Expression of EZH2, MBP-SUZ12, EED, RBBP4

   Depositor

   Chen Davidovich

   Article

   Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

   Insert

   EZH2, SUZ12, EED, RBBP4 (EZH2, SUZ12, RBBP4, EED Human)
   

   Expression

   Insect

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ134-STU-As

   Plasmid

   #138103

   Purpose

   Golden Gate entry vector for 4th crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMY.PRC2

   Plasmid

   #234582

   Purpose

   Expression of ProtA-EZH2, SUZ12, EED and RBBP4

   Depositor

   Chen Davidovich

   Article

   Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

   Insert

   EZH2, SUZ12, EED, RBBP4 (EZH2, SUZ12, RBBP4, EED Human)
   

   Expression

   Yeast

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ131-STU-Lb

   Plasmid

   #138096

   Purpose

   Golden Gate entry vector for 1st crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYPQ134-STU-Lb

   Plasmid

   #138105

   Purpose

   Golden Gate entry vector for 4th crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYPQ133-STU-Lb

   Plasmid

   #138102

   Purpose

   Golden Gate entry vector for 3rd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQ132-STU-Lb

    Plasmid

    #138099

    Purpose

    Golden Gate entry vector for 2nd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYPQ144-ZmUbi-pT

    Plasmid

    #138108

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs driven by Zea mays Ubi promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYPQ142-ZmUbi

    Plasmid

    #138106

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs driven by Zea mays Ubi promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNK5712

    Plasmid

    #219752

    Purpose

    pGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pGAP - nnH3H_v2 - tAOX
    

    Expression

    Yeast

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNK2657

    Plasmid

    #219741

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    hispidin- synthase from Mycena citricolor
    

    Use

    Synthetic Biology

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNK5867

    Plasmid

    #219751

    Purpose

    pGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pGAP - mcitHispS - tAOX
    

    Expression

    Yeast

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNK1508

    Plasmid

    #219750

    Purpose

    pGAP-like vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of GAP promoter, for yeast expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pGAP - npgA - tAOX
    

    Expression

    Yeast

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218914

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-T2A-SARS-CoV2-NSP7/BlastR (ORF1ab )
    

    Expression

    Mammalian

    Mutation

    2363G>C and 4330T>C

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNK3203

    Plasmid

    #219749

    Purpose

    DVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pCMV - mcitHispS - tSV40
    

    Expression

    Mammalian

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ143-ZmUbi

    Plasmid

    #138107

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs driven by Zea mays Ubi promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQ131-STU-As

    Plasmid

    #138094

    Purpose

    Golden Gate entry vector for 1st crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge