We narrowed to 8,409 results for: BLI;

1. AAV-SYN-mBaoJin-oPRE

   Plasmid

   #218683

   Purpose

   AAV vector plasmid expressing mBaoJin under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.

   Depositor

   Bart Nieuwenhuis

   Insert

   mBaoJin
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human synapsin promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pML104-KanMx4

   Plasmid

   #83476

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

   Depositor

   Patricia Wittkopp

   Insert

   KanMX4
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSaCas9_GFP

   Plasmid

   #64709

   Purpose

   Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells

   Depositor

   Kiran Musunuru

   Insert

   SaCas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP and 3xFLAG-SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEMB52/14-3-3_1_247

   Plasmid

   #40541

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   YWHAG (YWHAG Human)
   

   Use

   Baculovirus

   Tags

   6xHis

   Expression

   Mutation

   WT; aa1-247

   Promoter

   polH

   Available since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pJL31

   Plasmid

   #227428

   Purpose

   Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)

   Depositor

   Pierre Santucci

   Insert

   mWasabi
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJL32

   Plasmid

   #227429

   Purpose

   Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)

   Depositor

   Pierre Santucci

   Insert

   tdTomato
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Halo-TOMM20-N-10

   Plasmid

   #123284

   Purpose

   Localization: Outer Mitochondrial Membrane

   Depositor

   Kevin McGowan

   Insert

   TOMM20 (TOMM20 Human)
   

   Use

   Tags

   HaloTag7

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEM4Z-T7-5'UTR-mGreenLantern-3'UTR-A64

   Plasmid

   #203349

   Purpose

   Template for T7 in vitro transcription of mGreenLantern mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

   Depositor

   Christopher Grigsby, Molly Stevens

   Insert

   mGreenLantern
   

   Use

   Template for t7-mediated in vitro transcription o…

   Tags

   Flanked by 5' and 3' UTRs from Xenopus …

   Expression

   Mutation

   Promoter

   T7

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+) Drp1

   Plasmid

   #34706

   Depositor

   David Chan

   Insert

   Drp1 (Dnm1l Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. IRES-creERT2-FNF

    Plasmid

    #188230

    Purpose

    EMCV IRES-cre-ERT2-FRT-Neo*-FRT cassette

    Depositor

    Mario Capecchi

    Insert

    IRES-creERT2-FNF
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-mArg1-Flag

    Plasmid

    #34574

    Depositor

    Peter Murray

    Insert

    Arg1 (Arg1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pFLAG-6a-Ataxin3Q22

    Plasmid

    #22126

    Depositor

    Henry Paulson

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pCW57.1 CCNE1

    Plasmid

    #164144

    Purpose

    Doxycycline inducible expression of Cyclin E1

    Depositor

    Richard Possemato

    Insert

    CCNE1 (CCNE1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-phSyn-flex-SypEGFP

    Plasmid

    #153203

    Purpose

    Can be used to generate AAV virus that will mark the presynaptic terminal (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

    Depositor

    Lynette Lim

    Insert

    synaptophysin-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    rat synapsin

    Available since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

    Plasmid

    #99248

    Purpose

    single-vector system with EGFP reporter for use in CROPseq knockout screens

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pScarlessHD-sfGFP-DsRed

    Plasmid

    #80811

    Purpose

    Vector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Kate O'Connor-Giles

    Insert

    sfGFP-piggyBac-DsRed
    

    Use

    Unspecified

    Tags

    sfGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV14_Fibrillarin

    Plasmid

    #73061

    Purpose

    expression clone for human Fibrillarin with C-terminal 3xFLAG-tag

    Depositor

    Stefan Stamm

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. SARS-CoV Nucleocapsid

    Plasmid

    #154269

    Purpose

    Expresses myc-tagged nucleocapsid (N) from SARS-CoV (1) in mammalian cells

    Depositor

    Peter Klein

    Insert

    SARS-CoV Nucleocapsid
    

    Use

    Tags

    5X myc epitope

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGR596

    Plasmid

    #213787

    Purpose

    Integrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette

    Depositor

    Chang Liu

    Insert

    BadBoy2 TP-DNAP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    See manuscript

    Promoter

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTipi2.1-AZU_SP

    Plasmid

    #229144

    Purpose

    Allows for expression of recombinant antibodies. Includes human azurocidin signal peptide.

    Depositor

    Institute for Protein Innovation (IPI)

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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