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pFAST-BAC-Cdc20wt (2193)

Plasmid

#39850

Depositor

Jonathon Pines

Article

Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

Insert

Cdc20 (CDC20 Human)

Use

Baculovirus expression

Tags

His

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
Desmocollin2_tail-GST

Plasmid

#36992

Depositor

Kathleen Green

Article

Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

Insert

Dsc2 (DSC2 Human)

Tags

GST

Expression

Bacterial

Mutation

Intracellular domain of Dsc2 (nt 2,603–3,158)

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only
pT7-7 asyn S87A

Plasmid

#36058

Depositor

Hilal Lashuel

Article

Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

Insert

alpha-synuclein S87A (SNCA Human)

Expression

Bacterial

Mutation

S87A

Promoter

T7

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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TAL2364

Plasmid

#36777

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HDAC1-TALEN_Left (Hdac1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
KIFC1

Plasmid

#25253

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

KIFC1:G361-C715 (KIFC1 Human)

Use

Baculovirus expression

Tags

His

Mutation

Contains amino acids G361-C715

Promoter

polyhedrin

Available Since

Aug. 13, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pHIS2-TIP47 (Delta 1-190)

Plasmid

#17640

Depositor

James Hurley

Article

Hickenbottom et al Structure. 2004 Jul . 12(7):1199-207.

Insert

Tip47 (Delta 1-190) (Plin3 Mouse)

Tags

His

Expression

Bacterial

Mutation

Delta 1-190

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only
pCDH-V3-CD20-Puro

Plasmid

#209757

Purpose

Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 3 mRNA isoform.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2-FKBP-PIP5K1C_Kina-HD

Plasmid

#202741

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:FKBP1A(3-108):[GGSA]4GG:PIP5K1C(79-366)(D101R/R304D) (PIP5K1C Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBI-eGFP-Rab31

Plasmid

#200265

Purpose

Expression of eGFP-Rab31 (human) in mammalian cells

Depositor

Guangpu Li

Article

Banworth et al J Biol Chem. 2022 Sep;298(9):102281. doi

Insert

Rab31 (human cDNA) (RAB31 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

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pEasyG1_nat

Plasmid

#184911

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pPyCAG-NE-mKate2-IRES-Hph

Plasmid

#183616

Purpose

Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered mKate2 (NLS-mKate2-EmerinTMD). Confers hygromycin B resistance.

Depositor

Sally Lowell

Article

Blin et al PLoS Biol. 2019 Aug 9;17(8):e3000388. do

Insert

mKate2

Tags

Human EMD transmembrane domain and NLS

Expression

Mammalian

Promoter

CAG

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pET_SZ1-RGG-GFP-RGG

Plasmid

#124937

Purpose

Expression of SZ1-RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His

Expression

Bacterial

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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ORAI1-YFP T184M

Plasmid

#114181

Purpose

ORAI1 channel with C-terminal YFP, carrying the mutation T184M in the protein 3rd transmembrane domain, responsible for the channel overactivity and associated with tubular aggregate myopathy (TAM).

Depositor

Nicolas Demaurex

Article

Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

Insert

ORAI1-YFP T184M (ORAI1 Human)

Tags

YFP

Expression

Mammalian

Mutation

T184M

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-mCherry-hPMCA2x/b

Plasmid

#111571

Purpose

Expresses a plasma membrane calcium pump hPMCA2x/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)

Tags

mCherry

Expression

Bacterial

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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SPIN2B (SPIN2BA-c011)

Plasmid

#98238

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

SPIN2BA (SPIN2B Human)

Tags

TEV-His6-Flag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Intu

Plasmid

#65430

Purpose

For mammalian expression of EGFP-mouse Inturned

Depositor

Aimin Liu

Article

Zeng et al Dev Biol. 2010 Mar 15;339(2):418-28. doi

Insert

Inturned (Intu Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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dTomato_LD 0_CS M_tTA-BFP

Plasmid

#58875

Purpose

MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')

Use

AAV

Tags

EBFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

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TAL2368

Plasmid

#36779

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

NCOR1-TALEN_Left (Ncor1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2369

Plasmid

#36780

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

NCOR1-TALEN_Right (Ncor1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2219

Plasmid

#36678

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MUTYH-TALEN_Right (Mutyh Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only