We narrowed to 4,909 results for: Mos
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pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP
Plasmid#188899PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening elementDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9P35S-N
Plasmid#66191Purposeexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotercauliflower mosaic virus 35S promoter (P35S)Available sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b-DiCas7-11-His
Plasmid#234482PurposePlasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)DepositorInsertDiCas7-11
UseCRISPRTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9P35S-H
Plasmid#66189Purposeexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotercauliflower mosaic virus 35S promoter (P35S)Available sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)
Plasmid#224497PurposeGateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)DepositorInsertActin-Vhh-sfGFP-P2A-HA-tdiRFP-caax
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN113 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26
Plasmid#86233PurposeTargeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Neomycine selection. Auxin-inducible degron system.DepositorInsertosTir1
UseMouse TargetingTagsV5 tagExpressionMammalianMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcdna 3.1 DSG-3 Tension Sensor
Plasmid#182717Purposeexpresses DSG-3 TSmodDepositorInsertdesmoglein-3 (Dsg3 Mouse)
UseTagsExpressionMammalianMutationTSmod inserted between G726 and G727PromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9P35S-B
Plasmid#66190Purposeexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotercauliflower mosaic virus 35S promoter (P35S)Available sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAGT9054
Plasmid#219430PurposeTranscriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCSDepositorInsertLB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
UseCRISPR; Moclo compatible level 1 module (position…TagsPapE-4LF2ExpressionPlantMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
SaCas9 variant click editor (CE1) - pCMV-T7-PCV2-nSaCas9-EcKlenow (MLE194)
Plasmid#217801PurposeVariant CE1 construct with SaCas9, expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSaCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSaCas9(N580A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN395 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE targeting
Plasmid#92141PurposeTargeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection. Auxin-inducible degron system.DepositorInsertosTir1
UseMouse TargetingTagsV5 tagExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Top2A-5X Gly-Venus-T2A-Hygro
Plasmid#211115PurposeFor tagging the C-term of Top2A with VenusDepositorInsertsUseCRISPRTagsExpressionBacterial and MammalianMutationPromotern/aAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX GFP Mps1 wt
Plasmid#63702PurposeInducible expression of GFP-Mps1 fusion in mammalian cellsDepositorInsertMps1 (TTK Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromotertight TRE promoterAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T4DNApol (JO638)
Plasmid#208956PurposeA variant CE1 construct with T4 DNA polymerase (-exo, activity enhanced), expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-T4pol-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A), T4Pol(-exo;D189A/E191A/L412M)PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-NUP98a_IDR-EGFP
Plasmid#232766PurposeExpresses dCas9-NUP98a_IDR-EGFPDepositorInsertdCas9-NUP98a_IDR-EGFP (NUP98 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
HUHe variant click editor (CE1) - pCMV-T7-DCV-nCas9-EcKlenow (JO608)
Plasmid#208946PurposeA variant CE1 construct with DCV HUH endonuclease, expressed from CMV or T7 promoters.DepositorInsertDCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-TM-VC
Plasmid#135499PurposeMammalian expression of VC-fused and FLAG-tagged TAPBPR with MHC TMDepositorInsertTAPBPR (TAPBPL Human)
UseTagsLuminal/Extracellular FLAG epitope tag, Signal pe…ExpressionMammalianMutationswitched transmembrane domain with that of MHC-IPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCherryC1 VAP-B KD/MD
Plasmid#226410PurposeExpression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to mCherry in mammalian cellsDepositorInsertVAP-B (VAPB Human)
UseTagsHA, T7-Xpress tag, and mCherryExpressionMammalianMutationK87D/M89D mutant, unable to bind FFAT motifsPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29(+exo) (LM2938)
Plasmid#208957PurposeA variant CE1 construct with Phi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-Phi29(+exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A)PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only
