We narrowed to 8,417 results for: Ott

1. p2CT-His-MBP-Lbu_C2c2_D1078A

   Plasmid

   #91860

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbuCas13a (LbuC2c2) D1078A
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   D1078A

   Promoter

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2CT-His-MBP-Lbu_C2c2_N314A

   Plasmid

   #91858

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbuCas13a (LbuC2c2) N314A
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   N314A

   Promoter

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p2CT-His-MBP-Lbu_C2c2_K1080A

   Plasmid

   #91862

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbuCas13a (LbuC2c2) K1080A
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   K1080A

   Promoter

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BC-A1-016

   Plasmid

   #78704

   Purpose

   A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a weak RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. BC-A1-015

   Plasmid

   #78703

   Purpose

   A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. BC-A1-010

   Plasmid

   #78697

   Purpose

   A strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BC-A1-008

   Plasmid

   #78695

   Purpose

   A weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BC-A1-007

   Plasmid

   #78694

   Purpose

   A weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJBL-RNAINS4-C24A

   Plasmid

   #69462

   Purpose

   RNA-IN S4 C24A variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

   Depositor

   Julius Lucks

   Article

   Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

   Insert

   RNA-IN S4 with C24A mutation
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   C24A and mutations for S4 variant (from wt)

   Promoter

   J23119 and J23119 (sigma 70)

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJBL-RNAINS4-A25C

    Plasmid

    #69461

    Purpose

    RNA-IN S4 A25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

    Depositor

    Julius Lucks

    Article

    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

    Insert

    RNA-IN S4 with A25C mutation
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    A25C and mutations for S4 variant (from wt)

    Promoter

    J23119 and J23119 (sigma 70)

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJBL-RNAINS4-C24AA25C

    Plasmid

    #69463

    Purpose

    RNA-IN S4 C24AA25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

    Depositor

    Julius Lucks

    Article

    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

    Insert

    RNA-IN S4 with C24A, A25C mutations
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    C24A, A25C, and mutations for S4 variant (from wt)

    Promoter

    J23119 and J23119 (sigma 70)

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. dEos-Vinculin-14

    Plasmid

    #57568

    Purpose

    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Betzig et al Science. 2006 Sep 15;313(5793):1642-5. E

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    dEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pCRII-Topo Tmtc4

    Plasmid

    #45869

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

    Insert

    TMTC4 in situ probe (Tmtc4 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    nt 1220-1798 on BC031368

    Promoter

    Available since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pAAVscCBPITuDlet-7Gpluc7xlet7BT

    Plasmid

    #35651

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    TuD let-7
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRNA-U6-TuDmiR122 seedonly

    Plasmid

    #35667

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    TuD miR-122seedonly
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSpCas9(BB)-2A-GFP (PX458)

    Plasmid

    #48138

    Purpose

    Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70

    Plasmid

    #62897

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGGAselect

    Plasmid

    #195714

    Purpose

    pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. E-cadherin-GFP

    Plasmid

    #28009

    Depositor

    Jennifer Stow

    Article

    Miranda et al J Biol Chem. 2001 Jun 22. 276(25):22565-

    Insert

    E-cadherin (CDH1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-C3-hYAP1

    Plasmid

    #17843

    Depositor

    Marius Sudol

    Article

    Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge