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We narrowed to 22,191 results for: his

Showing: 7401 - 7440 of 22191 results

  1. pET29b(+)_gp1(A11V)

    Plasmid
    #225131
    Purpose
    Phage GIL01 protein gp1 (A11V) mutant with a single nucleotide substitution (C32T)
    Depositor
    Matej Butala
    Insert
    gp1 (A11V)
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Alanine 11 to Valine
    Promoter
    T7 promoter
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225131-plasmid-map-sequence-id-448443

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  2. pYES2/NTA/Leu-LegA7

    Plasmid
    #216518
    Purpose
    Expression of LegA7
    Depositor
    Ralph Isberg
    Insert
    Leg A7
    Use
    Tags
    HIS-tag
    Expression
    Bacterial and Yeast
    Mutation
    LegA7 WT
    Promoter
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216518-plasmid-map-sequence-id-430062

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  3. pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Plasmid
    #170789
    Purpose
    Expresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)
    Depositor
    Kalle Gehring
    Insert
    Toxoplasma gondii PRL
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lacI
    Available Since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    170789-plasmid-map-sequence-id-337771

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  4. pmCellFree_KA1_SARS-COV-2_N

    Plasmid
    #169401
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus N with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _N (N SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available Since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169401-plasmid-map-sequence-id-336866

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  5. pmCellFree_KA1_SARS-COV-2_ORF6

    Plasmid
    #169392
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF6 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF6 (ORF6 SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169392-plasmid-map-sequence-id-335430

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  6. STK10 (STK10A-c043)

    Plasmid
    #110255
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    STK10 (STK10 Human)
    Use
    Tags
    His-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 - lacO
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110255-plasmid-map-sequence-id-212331

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  7. UBH12 (3DB4)

    Plasmid
    #25171
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubh12.414.617:B9 (UHRF1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    contains residues 126-285
    Promoter
    Available Since
    April 4, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. 4Z31

    Plasmid
    #69958
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RC3H2:YTC020-E10:C234061 (RC3H2 Human)
    Use
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. 4Z0O

    Plasmid
    #69954
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ZCWPW2:JMC01B-H12:C203050 (ZCWPW2 Human)
    Use
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. 4YVD

    Plasmid
    #69960
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PLRG1:JMC102-C09:C233452 (PLRG1 Human)
    Use
    Baculovirus expression
    Tags
    His and TEV cleavage site
    Expression
    Mutation
    Promoter
    polyhedrin
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. 4MYI

    Plasmid
    #60103
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PVX_084705:MAC04G-C11:C228845
    Use
    Baculovirus expression
    Tags
    6x His and TEV cleavage site
    Expression
    Mutation
    See comments
    Promoter
    polyhedrin
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. 4IIM

    Plasmid
    #51325
    Depositor
    Cheryl Arrowsmith
    Insert
    4IIM (ITSN1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available Since
    Feb. 24, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. 4IIO

    Plasmid
    #51326
    Depositor
    Cheryl Arrowsmith
    Insert
    4IIO (ITSN2 Human, 4IIO)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available Since
    Feb. 24, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. PTPRJ

    Plasmid
    #38888
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRJ (PTPRJ Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    C1239S inactivating mutation
    Promoter
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. CDY

    Plasmid
    #25507
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CDY (CDY2B Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    catalytic domain
    Promoter
    Available Since
    Sept. 10, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. SGF29 (3MEU)

    Plasmid
    #25317
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SGF29_6 (SGF29 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids 115-293
    Promoter
    Available Since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. SGF29 (3LX7)

    Plasmid
    #25314
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SGF29_3 (SGF29 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids 138-291
    Promoter
    Available Since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. C6orf197

    Plasmid
    #25258
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    C6orf197:M178-R505 (TBC1D22B Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids M178-R505
    Promoter
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. PLXND1 (3H6N)

    Plasmid
    #25187
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PLXND1:A1553-D1656 (PLXND1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids A1553-D1656
    Promoter
    Available Since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. PP-CDPK1 (3DXN)

    Plasmid
    #25129
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    541.m00134:MAC02T-C05:C40091
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids L72-Q339
    Promoter
    Available Since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  21. pCJ190

    Plasmid
    #162667
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162667-plasmid-map-sequence-id-321443

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  22. pCJ189

    Plasmid
    #162666
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162666-plasmid-map-sequence-id-321465

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  23. pCJ202

    Plasmid
    #162675
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    W159C, S238C; codon optimized for expression in E…
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162675-plasmid-map-sequence-id-321470

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  24. pOPINB-HOIL-1 helix-RBR (233–510)

    Plasmid
    #193859
    Purpose
    Expression of human His-tagged HOIL-1 (RBCK1) helix-RBR (aa 233–510) codon optimized for E. coli
    Depositor
    Bernhard Lechtenberg
    Article
    Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:
    Insert
    HOIL-1 (RBCK1 Human)
    Use
    Tags
    3C protease cleavage site and 6x-His tag
    Expression
    Bacterial
    Mutation
    Residues 233–510, codon optimised for expression …
    Promoter
    T7
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    193859-plasmid-map-sequence-id-379616

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  25. pCJ191

    Plasmid
    #162668
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162668-plasmid-map-sequence-id-321523

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  26. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid
    #108362
    Purpose
    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans
    Depositor
    Luisa Cochella
    Article
    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461
    Inserts
    Ath-HEN1 (HEN1 Mustard Weed, Codon optimised for C. elegans)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    Use
    Tags
    2xFlag, MYC, T2A peptide, and TY1 linker
    Expression
    Worm
    Mutation
    Promoter
    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…
    Available Since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    108362-plasmid-map-sequence-id-209815

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  27. pCJ206

    Plasmid
    #162679
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    E226T; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162679-plasmid-map-sequence-id-321456

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  28. pCJ211

    Plasmid
    #162684
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with deletion of the predicted signal peptide (Lys2:Gly19), with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) without 19 residue signal peptide
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaK2:G19; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162684-plasmid-map-sequence-id-321526

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  29. pCJ197

    Plasmid
    #162670
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    S131G; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162670-plasmid-map-sequence-id-321452

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  30. ABL1-N-HiBiT kinase domain_v1

    Plasmid
    #207121
    Purpose
    Bacterially expressed HiBiT-ABL1 kinase domain (D233-S500) for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Human Abelson Kinase Domain N-terminal HiBiT, N-terminal 10X His-tag (ABL1 Human)
    Use
    Tags
    HiBiT Tag and His-tag with thrombin site
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    207121-plasmid-map-sequence-id-422565

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  33. SHIP2-C1

    Plasmid
    #214905
    Purpose
    expression of the truncated variants of the SHIP2 that retains second proline-rich domain and C-terminal sterile alpha motif
    Depositor
    Pavel Krejčí
    Article
    Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608
    Insert
    human SHIP2 aa 740-1258 (INPPL1 Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    delta 1-739aa
    Promoter
    CMV
    Available Since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214905-plasmid-map-sequence-id-426097

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  36. pCJ205

    Plasmid
    #162676
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224A an
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    C224A, C529A; codon optimized for expression in E…
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162676-plasmid-map-sequence-id-320828

    Enlarge

  37. pCJ198

    Plasmid
    #162671
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating F495I mutation.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    F495I; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162671-plasmid-map-sequence-id-321447

    Enlarge

  38. pCJ196

    Plasmid
    #162669
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating catalytic mutation, S225A
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    S225A; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162669-plasmid-map-sequence-id-321445

    Enlarge

  39. pCJ204

    Plasmid
    #162677
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224H and C529F mutations.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    C224H, C529F; codon optimized for expression in E…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162677-plasmid-map-sequence-id-321439

    Enlarge

  40. pCJ201

    Plasmid
    #162674
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224W and C529SS mutations.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    C224W, C529SS; codon optimized for expression in …
    Promoter
    T7/lac
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162674-plasmid-map-sequence-id-320830

    Enlarge

Showing: 7401 - 7440 of 22191 results